In vitro antileishmanial and antitrypanosomal activity of compounds isolated from the roots of Zanthoxylum tingoassuiba

Abstract

Five coumarins (5,7,8-trimethoxycoumarin (1), sabandin (2), cubreuva lactone (3), 5,7- dimethoxycoumarin (5) and braylin (6)), seven furoquinoline alkaloids (isopimpinelin (4), pteleine (7), maculine (8), skimianine (10), robustine (11), y-fagarine (12) and dictamine (13) and the furofuran type lignin syringaresinol (9)) have been identified for the first time in the roots of Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae. Pure compounds 1, 6, 9, 12 were tested against Leishmania amazonensis parasites and epimastigotes forms of Trypanosoma cruzi. All the tested products displayed an antiparasitic activity similar to that of the positive controls (benznidazole and amphotericin B). Compound 9 was the most active against both parasites with ICO50 values of 11.98 μM and 7.55 μM against L. amazonensis and T. cruzi, respectively.

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Correspondence to Rafael S. Costa.

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Authors’ contributions

RSC contributed in collecting plant sample, running the laboratory work, data analysis and drafted the paper. OPSF contributed in collecting plant sample, running the laboratory work. OCSDJ and JJS contributed to biological studies. MLH contributed to data analysis and drafted the paper. MAVS and EV designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Costa, R.S., Souza Filho, O.P., Dias Júnior, O.C.S. et al. In vitro antileishmanial and antitrypanosomal activity of compounds isolated from the roots of Zanthoxylum tingoassuiba. Rev. Bras. Farmacogn. 28, 551–558 (2018). https://doi.org/10.1016/j.bjp.2018.04.013

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Keywords

  • Leismaniasis
  • Trypansomiasis
  • Coumarins
  • Furoquinoline alkaloids
  • Syringaresinol