Development and clinical efficacy evaluation of anti-greasy green tea tonner on facial skin


Green tea (Camellia sinensis (L.) Kuntze, Theaceae) polyphenols have activities against skin disorders; however, anti-sebum efficacy of green tea cosmetic has sparely been reported. The facial tonner containing green tea was therefore developed and clinical evaluated. The base formulas which stabled following accelerated tests were sensorial assessed in ten volunteers. The base with hydroxyethyl cellulose, glycerin and panthenol (totally 3.6%) with the significant (p < 0.05) preference (82.3 ± 0.55%) over the others was further developed to green tea preparations. All of the products were stable and caused none of skin irritation as closed patch tested in twenty volunteers. Thereafter, they were clinical evaluated in the same group of the volunteers and monitored with Sebumeter® by means of a split-face, randomized single-blind, placebo-controlled study. Anti-greasy efficacy of 2, 4.5 and 7% green tea tonners were 3.47 ± 0.10, 8.18 ± 0.44 and 17.87 ± 0.46% following 14 days of facial treatment. The efficiency was pronounced at the end of the study; day 28 (8.48 ±0.13, 20.26 ± 1.03 and 31.57 ± 1.22%). Anti-sebum efficacy of the 4.5 and 7% green tea tonners were significantly better than the base formula (day 14; p < 0.05, day 28; p < 0.01). The efficacy of 28 days treatment was significantly better than 14 days (p < 0.05). The safe and efficient green tea tonner for oily face treatment was therefore approved in this context.


  1. Aburjai, T., Natsheh, F.M., 2003. Plants used in cosmetics. Phytother. Res. 17, 987–1000.

    Article  PubMed  Google Scholar 

  2. Chaisripipat, W., Lourith, N., Kanlayavattanakul, M., 2015. Anti-dandruff hair tonic containing lemongrass (Cymbopogonflexuosus) oil. Forsch. Komplementmed. 22, 226–229.

    PubMed  Google Scholar 

  3. Chuarienthong, P., Lourith, N., Leelapornpisid, P., 2010. Clinical efficacy comparison of anti-wrinkle cosmetics containing herbal flavonoids. Int. J. Cosmet. Sci. 32, 99–106.

    CAS  Article  PubMed  Google Scholar 

  4. Crowther, J.M., 2016. Method for quantification of oils and sebum levels on skin using the Sebumeter®. Int. J. Cosmet. Sci. 38, 210–216.

    CAS  Article  PubMed  Google Scholar 

  5. Dobrev, H., 2007. Clinical and instrumental study of the efficacy of a new sebum control cream. J. Cosmet. Dermatol. 6, 113–118.

    Article  PubMed  Google Scholar 

  6. Elmets, C.A., Singh, D., Tubesing, K., Matsui, M., Katiyar, S., Mukhtar, H., 2001. Cutaneous photoprotection from ultraviolet injury by green tea polyphenols. J. Am. Acad. Dermatol. 44, 425–432.

    CAS  Article  PubMed  Google Scholar 

  7. Hsu, S., 2005. Green tea and the skin. J. Am. Acad. Dermatol. 52, 1049–1059.

    Article  PubMed  Google Scholar 

  8. Kanlayavattanakul, M., Lourith, N., 2011. Body malodours and theirtopical treatment agents. Int. J. Cosmet. Sci. 33, 298–311.

    CAS  Article  PubMed  Google Scholar 

  9. Kartiyar, S.K., Elmets, C.A., 2001. Green tea polyphenols antioxidants and skin photoprotection. Int. J. Oncol. 18, 1307–1313.

    Google Scholar 

  10. Lourith, N., Kanlayavattanakul, M., 2010. Oral malodor and active ingredients for treatment. Int. J. Cosmet. Sci. 32, 321–329.

    CAS  Article  PubMed  Google Scholar 

  11. Lourith, N., Kanlayavattanakul, M., 2013. Hairloss and herbs fortreatment. J. Cosmet. Dermatol. 12, 210–212.

    Article  PubMed  Google Scholar 

  12. Mahmood, T., Akhtar, N., Khan, B.A., Khan, H.M.S., Saeed, T., 2010. Outcomes of 3% green tea emulsion on skin sebum production in male volunteers. Bosn. J. Basic Med. Sci. 10, 260–264.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Mahmood, T., Akhtar, N., Moldovan, C., 2013. A comparison of the effects of topical green tea and lotus on facial sebum control in healthy humans. Hippokratia 17, 64–67.

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Peirano, R.I., Hamann, T., Düsing, H.J., Akhiani, M., Koop, U., Schmidt-Rose, T., Wenck, H., 2011. Topically applied L-carnitine effectively reduces sebum secretion in human skin. J. Cosmet. Dermatol. 11, 30–36.

    Article  Google Scholar 

  15. Piérard-Franchimont, C., Quatresooz, P., Piérard, G.E., 2010. Sebum production. In: Farage, M.A., Miller, K.W., Maibach, H.I. (Eds.), Textbook of Aging Skin. Springer, Berlin, pp. 343–352.

    Google Scholar 

  16. Sakuma, T.H., Maibach, H.I., 2012. Oily skin: an overview. Skin Pharmacol. Physiol. 25, 227–235.

    Article  PubMed  Google Scholar 

  17. Schnuch, A., Aberer, W., Agathos, M., Becker, D., Brasch. J., Eisner, P., Frosch, P.J., Fuchs, T., Geier, J., Hillen, U., Löffler, H., Mahler, V., Richter, G., Szliska, C., 2008. Performing patch testing with contact allergens. J. Dtsch. Dermatol. Ges. 6, 770–775.

    PubMed  Google Scholar 

  18. Sharquie, K.E., Noaimi, A.A., Al-Hashimy, S.A., Al-Salih, M.M., 2014. Therapeutic evaluation of 2% tea lotion in comparison with 5% zinc sulphate solution in the treatment of acne rosacea. J. Cosmet. Dermatol. Sci. Appl. 4, 60–65.

    CAS  Google Scholar 

  19. Wu, Y., Niu, Y., Zhong, S., Liu, H., Zhen, Y., Saint-Leger, D., Verschoore, M., 2013. A preliminary investigation of the impact of oily skin on quality of life and concordance of self-perceived skin oiliness and skin surface lipids (sebum). Int. J. Cosmet. Sci. 35, 442–447.

    CAS  Article  PubMed  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Nattaya Lourith.

Additional information

Authors’ contributions

PM (MSc student) contributed in all the experimental work, analyzed the data. MK contributed to project plaining and drafting of the manuscript. NL designed the study and contributed to critical reviewing of the manuscript. All the authors have read the final manuscript and approved the submission.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Meetham, P., Kanlayavattanakul, M. & Lourith, N. Development and clinical efficacy evaluation of anti-greasy green tea tonner on facial skin. Rev. Bras. Farmacogn. 28, 214–217 (2018).

Download citation


  • Formulation
  • Stability
  • Green tea
  • Sebum
  • Tonner
  • Phytocosmetics