Chromatographic profiles of extractives from leaves of Eugenia uniflora

Abstract

Eugenia uniflora L., Myrtaceae, popularly known as “pitanga”, is used in traditional medicine due the properties attributed to its chemical content, these being mainly hydrolysable tannins and flavonoids. This study provides a qualitative and quantitative evaluation of chemical profile from leaves of E. uniflora. The HPLC analysis was carried out on a C18 column (4.6 mm × 250 mm, 5 μm) by gradient elution with methanol and water (acidified with trifluoracetic acid); and silica gel Plates 60-F254 with 10–12 μm and 5–6 μm particles, respectively for TLC and High HPTLC analysis. The chromatographic data obtained from HPLC, TLC and HPTLC presented bands and peaks related to flavonoids (myricitrin and derivatives) and tannins (gallic and ellagic acids), which were observed from different samples. The chromatographic similarities enabled the building of a typical fingerprint for the herbal material. The similarity analysis of the sample data by Pearson correlation showed R values >0.9 among peaks (HPLC) and bands (HPTLC). In addition, the analytical methodology developed by HPLC enabled the satisfactory quantification of marker substances [ellagic acid = 0.22% and 0.20% (m/m); gallic acid = 0.20% and 0.43%; myricitrin = 0.42 and 1.74% (m/m) in herbal drug and crude extract, respectively]. The procedure was also validated in accordance with the assays required by Brazilian legislation. Thus, the HPTLC and HPLC methods developed in this study provide helpful and simple tools for the quality evaluation both qualitatively and quantitatively of raw materials and extractives from leaves of E. uniflora.

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Correspondence to Luiz A. L. Soares.

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Authors’ contributions

ICFB (Ph.D. student) collected and dried the plant material samples, worked on laboratorial tests, conducted all analysis and wrote the article. RTMR (undergraduate student) assisted in laboratory work in all experiments. MRAF assisted in the project design and reviewed the manuscript. LALS was responsible for the project concept and supervision of the study, as well as the writing and review of the manuscript.

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Bezerra, I.C.F., de Ramos, R.T.M., Ferreira, M.R.A. et al. Chromatographic profiles of extractives from leaves of Eugenia uniflora. Rev. Bras. Farmacogn. 28, 92–101 (2018). https://doi.org/10.1016/j.bjp.2017.11.002

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Keywords

  • Chromatographic fingerprint
  • Eugenia uniflora
  • HPLC
  • HPTLC