UPLC/Q-TOF-MS profiling of phenolics from Canarium pimela leaves and its vasorelaxant and antioxidant activities

Abstract

Canarium pimela K.D. Koenig, Burseraceae, have a long history of use in the Chinese traditional medicine treatment of various ailments including hypertension, and our research team has reported the antihypertensive activity and delineated the mechanism involved in the action. The following research aims to evaluate the vasorelaxant and antioxidant activities of ethanol extract from C. pimela leaves and to analyze its chemical composition by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) that may correlate with their pharmacological activities. The results showed that pre-incubation of aortic rings with the extract (0.3, 1, 3, 10, 30 and 100 mg/l) significantly inhibited the contractile response of the rings to norepinephrine-induced contraction (p < 0.01or p < 0.05). Crude ethanol extract and refined ethanol extract showed a highest inhibitory effect against 2,2dipheyl- 2-picrylhydrazyl hydrate scavenging activity (IC50 of crude ethanol extract = 15.42±0.14 μg/ml and IC50 of refined ethanol extract = 5.72±0.31 μg/ml) and 2,2′-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid ammonium salt) (ABTS (IC50 of crude ethanol extract = 3.24±0.18 μg/ml and IC50 of refined ethanol extract = 1.88±0.07 μg/ml) scavenging activity, which was considerably higher than that reported for butylated hydroxytoluene and lower of that measured for ascorbic acid. Moreover, its chemical composition was analyzed by UPLC/Q-TOF-MS. Sixteen compounds including nine flavonoids, four tannins, two phenolic acids and one dianthrone were identified for the first time as constituents of this species. And of this, six major phenolic components were simultaneous quantitative analysis by HPLC-UV, chlorogenic acid is the major compounds in C. pimela leaves. These results indicate that the phenolic-rich extract of C. pimela leaves is a promising natural pharmaceutical for combating hypertension and oxidative stress.

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Correspondence to Qingchun Xie or Zhufen Lv.

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Authors’ contributions

YFD and YLL collected and identified the samples. JW and YY performed the experiments interpreted the results, and JW drafted the manuscript. XAF conducted the UPLC/Q-TOF-MS analysis and designed the study. QCX and YZC supervised the laboratory work. ZFL and JFB interpreted the results and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Wu, J., Fang, X., Yuan, Y. et al. UPLC/Q-TOF-MS profiling of phenolics from Canarium pimela leaves and its vasorelaxant and antioxidant activities. Rev. Bras. Farmacogn. 27, 716–723 (2017). https://doi.org/10.1016/j.bjp.2017.10.005

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Keywords

  • Phenolic
  • UPLC/Q-TOF-MS
  • Vasorelaxant
  • Antioxidant
  • Biological activity
  • HPLC-DAD