Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus

Abstract

This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise,accurate and robust. The response was linear over a range of 5–200 μg/ml (r > 0.999). The range of recoveries was 92.27–102.54%. The relative standard deviation values for intra- and inter-day precision studies were 4.30 and 3.78%, respectively. This assay can be readily utilized as quality control method for catechin in the dried extract of P. pseudocaryophyllus.

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Correspondence to Joelma A. M. Paula.

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Authors’ contributions

LCS contributed by running the laboratory work, chromatographic analysis, and analysis of the data and also drafted the paper. RDM contributed to the laboratory work and chromatographic analysis. DRS contributed to chromatographic analysis. VCSA contributed to critical reading of the manuscript. JRP and ECC contributed by providing the standard catechin and with the critical reading of the manuscript. JAMP contributed by running laboratory work, and designed the study, supervised the laboratory work and contributed to the drafting and critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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The authors declare no conflicts of interest.

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Silva, L.C., Machado, R.D., Silva, D.R. et al. Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus. Rev. Bras. Farmacogn. 27, 645–649 (2017). https://doi.org/10.1016/j.bjp.2017.07.002

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Keywords

  • HPLC-PDA
  • Citral chemotype
  • Quality control
  • Chemical marker