Moringa oleifera Lam., Moringaceae, and Telfairia occidentalis Hook. f., Curcubitaceae, leaves are two tropical vegetables of medicinal properties. In this study, the inhibitory activities and the radical scavenging potentials of these vegetables on relevant enzymes of type 2-diabetes (α-amylase and α-glucosidase) were evaluated in vitro. HPLC-DAD was used to characterize the phenolic constituents and Fe2+-induced lipid peroxidation in rat’s pancreas was investigated. Various radical scavenging properties coupled with metal chelating abilities were also determined. However, phenolic extracts from the vegetables inhibited α-amylase, α-glucosidase and chelated the tested metals (Cu2+ and Fe2+) in a concentration-dependent manner. More so, the inhibitory properties of phenolic rich extracts from these vegetables could be linked to their radical scavenging abilities. Therefore, this study may offer a promising prospect for M. oleifera and T. occidentalis leaves as a potential functional food sources in the management of type 2-diabetes mellitus.
Adefegha, S. A., Oboh, G., 2012. Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices. Pharm. Biol. 50, 857–865.
Afifi, A.F., Kamel, E.M., Khalil, A.A., Foaad, M.A., Fawziand, E.M., Houseny, M., 2008. Purification and characterization of alphα-amylase from Penicillium olsonii under the effect of some antioxidant vitamins. Glob. J. Biotechnol. Biochem. 3, 14–21.
Akindahunsi, A.A., Oboh, G., 1999. Effect of some post-harvest treatments on the bioavailability of zinc from some selected tropical vegetables. La Riv. Ital. Delle Sost. Grasse 76, 285–287.
Anokwuru, C.P., Ajibaye, O., Adesuyi, A., 2011. Comparative antioxidant activity of water extract of Azadiractha indica stem bark and Telfairia occidentalis leaf. Curr. Res. J. Biol. Sci. 3, 430–434.
Apostolidis, E., Kwon, Y.I., Shetty, K., 2007. Inhibitory potential of herb, fruit, and fungal enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Inn. Food Sci. Emerg. Technol. 8, 46–54.
Atawodi, S.E., Atawodi, J.C., Idakwo, G.A., Pfundstein, B., Haubner, R., Wurtele, G., Bartsch, H., Owen, R.W., 2010. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J. Med. Food 13, 710–716.
Belle, N.A.V., Dalmolin, G.D., Fonini, G., Rubim, M.A., Rocha, J.B.T., 2004. Polyamines reduce lipid peroxidation induced by different prooxidant agents. Brain Res. 1008, 245–251.
Chen, X., Zheng, Y., Shen, Y., 2006. Voglibose (Basen, AO-128), one of the most important glucosidase inhibitors. Curr. Med. Chem. 13, 109–116.
Cheplick, S., Kwon, Y., Bhowmik, P., Shetty, K., 2010. Phenolic-linked variation in strawberry cultivars for potential dietary management of hyperglycemia and related complications of hypertension. Bioresour. Technol. 101, 404–413.
Chiasson, J.L., 2006. Acarbose forthe prevention of diabetes, hypertension, and cardiovascular disease in subjects with impaired glucose tolerance: the study to prevent non-insulin-dependent diabetes mellitus (STOP-NIDDM) trial. Endocr. Pract. 1, 25–30.
Chu, Y., Sun, J., Wu, X., Liu, R.H., 2002. Antioxidant and antiproliferative activity of common vegetables. J. Agric. Food Chem. 50, 6910–6916.
Dieye, A.M., Sarr, A., Diop, S.N., Ndiaye, M., Sy, G.Y., Diarra, M., Rajraji Gaffary, I., Ndiaye Sy, A., Faye, B., 2008. Medicinal plants and the treatment of diabetes in Senegal: survey with patients. Fundam. Clin. Pharmacol. 22, 211–216.
Duckworth, W.C., 2001. Hyperglycemia and cardiovascular disease. Curr. Atheroscler. 3, 383–391.
Finefrock, A.E., Bush, A.I., Doraiswamy, P.M., 2003. Current status of metals as therapeutic targets in Alzheimer’s disease. J. Am. Ger. Soc. 51, 1143–1148.
Fraga, C.G., Oteiza, P.I., 2002. Iron toxicity and antioxidant nutrients. Toxicology 180, 23–32.
Ghimeray, A.K., Jin, C., Ghimine, B.K., Cho, D.H., 2009. Antioxidant activity and quantitative estimation of azadirachtin and nimbin in Azadirachtaindica A, Juss grown in foothills of Nepal. Afr. J. Biotechnol. 8, 3084–3091.
Giridhari, V.A., Malathi, D., Geetha, K., 2011. Anti-diabetic property of drumstick (Moringa oleifera) leaf tablets. Int. J. Health Nutr. 2, 1–5.
Guevara, A.P., Vargas, C., Sakural, H., Fujiwara, Y., Hashimoto, K., Maoka, T., Kuzuka, M., Ito, Y., Tokuda, H., Hishino, H., 1999. An antitumor promoter from Moringa oleifera Lam. Mutat. Res. 440, 181–188.
Gyamfi, M.A., Yonamine, M., Aniya, Y., 1999. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen. Pharmacol. 32, 661–667.
Hsu, B., Coupar, I.M., Ng, K., 2006. Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem. 98, 317–328.
Ikpeme, E.V., Ekaluo, U.B., Udensi, O.U., Ekerette, E.E., 2014. Screening fresh and dried fruits of avocado pear (Perseaamericana) for antioxidant activities: an alternative for synthetic antioxidant. J. Life Sci. Res. Discov. 1, 19–25.
Islam, M.M., 2013. Biochemistry, medicinal and food values of jute (Corchorus capsularis L. and C olitorius L.) leaf: a review. Int. J. Enhanc. Res. Sci.Technol. Eng. 2, 135–144.
Jaiswal, D., Kumar Rai, P., Kumar, A., Mehta, S., Watal, G., 2009. Effect of Moringa oleifera Lam. leaves aqueous extract therapy on hyperglycemic rats. J. Ethnopharmacol. 123, 392–396.
Kar, A., Choudhary, B.K., Bandyopadhyay, N.G., 2003. Comparative evaluation of hypoglycemic activity of some Indian medicinal plants in alloxan diabetic rats. J. Ethnopharmacol. 84, 105–108.
Kasolo, J.N., Bimenya, G.S., Ojok, L., Ochleng, J., Ogwal-Okeng, J.W., 2010. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J. Med. Plant Res. 4, 753–757.
Kumar, S.P., Debasis, M., Goutam, G., Panda, C.S., 2010. Medicinal uses and pharmacological properties of Moringa oleifera. Int. J. Phytomed 2, 210–216.
Kumari, D.J., 2010. Hypoglycemic effect of Moringa oleifera and Azadirachta indica in type-2 diabetes. Bioscan 5, 211–214.
Kurma, S.R., Mishra, S.H., 1998. Antiinflammatory and hepatoprotective activities of fruits of Moringa pterygosperma Gaerth. Ind. J. Nat. Prod. 14, 3–10.
Kwon, Y.I., Apostolidis, E., Kim, Y.C., Shetty, K., 2007. Health benefits of traditional corn, beans and pumpkin: in vitro studies for hyperglycemia and hypertension management. J. Med. Food 10, 266–275.
Meda, A., Lamien, C.E., Romito, M., Millogo, J., Nacoulma, O.G., 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Faso honey, as well as their radical scavenging activity. J. Food Chem. 91, 571–587.
Minnoti, G., Aust, S.D., 1987. An investigation into the mechanism of citrate Fe2+ dependent lipid peroxidation. Free Radic. Biol. Med. 3, 379–387.
Monera, T.G., Maponga, C.C., 2010. Moringa oleifera supplementation by patients on antiretroviral therapy. J. Int. AIDS Soc. 13, 188.
Nwozo, S.O., Adaramoye, O.A., Ajaiyeoba, E.O., 2004. Anti-diabetic and hypolipidemic studies of Telifairia occidentalis on alloxan induced diabetic rabbits. Nigerian J. Natl. Prod. Med. 8, 37–39.
Oboh, G., Agunloye, O.M., Adefegha, S.A., Akinyemi, A.J., Ademiluyi, A.O., 2015. Caffeic and chlorogenic acids inhibit key enzymes linked to type-2 diabetes (in vitro): a comparative study. J. Basic Clin. Physiol. Pharmacol. 26, 165–170.
Oboh, G., Rocha, J.B.T., 2007. Antioxidant in Foods: A New Challenge for Food Processors: Leading Edge Antioxidants Research. Nova Science Publishers Inc, New York, pp. 35–64.
Ohkawa, H., Ohishi, N., Yagi, K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ann. Biochem. 95, 351–358.
Oyaizu, M., 1986. Studies on products of browning reaction: antioxidative activity of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44, 307–315.
Pereira, R.P., Boligon, A.A., Appel, A.S., Fachinetto, R., Ceron, C.S., Tanus-Santos, J.E., Athayde, M.L., 2014. Chemical composition, antioxidant and anticholinesterase activity of Melissa officinalis. Ind. Crop. Prod. 53, 34–45.
Puntel, R.L., Nogueira, C.W., Rocha, J.B.T., 2005. Krebs cycle intermediates modulate thiobarbituric reactive species (TBARS) production in Rat Brain In vitro. Neurochem. Res. 30, 225–235.
Sangeetha, R., Vedasree, N., 2012. In vitro α-amylase inhibitory activity of the leaves of Thespesia populnea. Int. Scholar. Res. Notices, https://doi.org/10.5402/2012/515634.
Shukla, S., Mehta, A., Bajpai, V.K., Shukla, S., 2009. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem. Toxicol. 47, 2338–2343.
Siddaraju, M.N., Dharmesh, S.M., 2007. Inhibition of gastric H+, K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Curcuma amada. J. Agric. Food Chem. 55, 7377–7386.
Singleton, V.L., Orthofor, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Method Enzymol. 299, 152–178.
Talhiliani, P., Kar, A., 2000. Role of Moringa oleifera leaf extract in the regulation of thyroid hormone status in adult male and female rats. Pharmacol. Res. 41, 319–323.
Torres-Fuentes, W.P., Benson, G.S., McConnell, J., 2011. Role of copper and oxidative stress in cardiovascular diseases. Ann. Biol. Res. 1, 158–173.
Tripathy, S., Pradhan, D., Anjana, M., 2010. Antiinflammatory and antiarthritic potential of Ammania baccifera Linn. Int. J. Pharm. Biosci. 1, 1–7.
Udenigwe, C.C., Adebiyi, A.P., Doyen, A., Bazinet, L., Aluko, R.E., 2012. Low molecu-larweight flaxseed protein-derived arginine-containing peptides reduced blood pressure of spontaneously hypertensive rats faster than amino acid form of arginine and native flaxseed protein. Food Chem. 132, 468–475.
Uyoh, E.A., Chukwura, P.N., David, I.A., Bassey, A.C., 2013. Evaluation of antioxidant capacity of two Ocimum species consumed locally as spices in Nigeria as a justification for increased domestication. Am. J. Plant Sci. 4, 222–230.
Wadkar, K.A., Magdum, C.S., Patil, S.S., Naikwade, N.S., 2008. Anti-diabetic potential and Indian medicinal plants. J. Herbal Med. Toxicol. 2, 45–50.
Worthington, V., 1993. Alpha amylase. In: Worthington, V. (Ed.), Worthington Enzyme Manual. Worthington Biochemical Corp, Freehold, NJ, pp. 36–41.
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Jimoh, T.O. Enzymes inhibitory and radical scavenging potentials of two selected tropical vegetable (Moringa oleifera and Telfairia occidentalis) leaves relevant to type 2 diabetes mellitus. Rev. Bras. Farmacogn. 28, 73–79 (2018). https://doi.org/10.1016/j.bjp.2017.04.003
- Type 2-diabetes
- Lipid peroxidation