Antispasmodic activity from Serjania caracasana fractions and their safety

Abstract

In a previous study, we reported the antispasmodic and gastroprotective effects of the Serjania caracasana (Jacq.) Willd., Sapindaceae, extract. In the present study, we evaluated the LD50, hemolytic and antispasmodic activities of its fractions and characterized its major constituents by isolation and GC-MS. The animals showed non-toxic symptoms with oral doses up to 2000 mg/kg, suggesting a safe oral administration. Furthermore, a low hemolytic activity was detected for the saponin fraction. Antispasmodic activity of the fractions was evaluated through carbachol-induced contractions in rat ileum. The hexane fraction was the most potent (IC50 68.4 ± 5.9 μg/ml) followed by the dichloromethane fraction (IC50 161.3.4±40.7 μg/ml). Butanol fraction was the less effective (IC50 219.8±60.3 μg/ml). The phytochemical study of the S. caracasana fractions afforded the isolation of friedelin, β-amyrin, allantoin and quercitrin. This is the first time that the presence of allantoin and quercitrin in the Serjania genus has been reported. Among the isolated compounds and those characterized by GC-MS, β-amyrin and β-sitosterol were present in the most active fractions, hexane and dichloromethane, and they may be related to its antispasmodic activity. In addition, spathulenol was only found in the hexane fraction and its presence might justify the highest antispasmodic activity observed for this fraction.

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Correspondence to Paulo R. H. Moreno.

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Authors’ contributions

FLS (Ph.D. student) contributed by conducting the phytochemical laboratory work, the hemolytic evaluation and drafting the paper. JLVS contributed by conducting the pharmacological assays, teaching JMS and LSAM in the conducting of the pharmacological assays and by critical reading of the manuscript. VLAN supervised the pharmacological work and contributed to critical reading of the manuscript. MY contributed with the characterizing of many isolated secondary metabolites. PHV contributed determining the EI-HRMS of allantoin. MNE supervised the laboratory work and contributed to critical reading of the manuscript. JMBF designed the phytochemical work and contributed guiding and supporting the plant collection and the laboratory work. PRHM contributed supporting and designing the phytochemical work and the hemolytic assays, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Silva, F.L., da Silva, J.L.V., Silva, J.M. et al. Antispasmodic activity from Serjania caracasana fractions and their safety. Rev. Bras. Farmacogn. 27, 346–352 (2017). https://doi.org/10.1016/j.bjp.2016.12.002

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Keywords

  • Antispasmodic activity
  • Compounds isolation
  • GC-MS
  • Hemolytic assay
  • Ileum rat
  • Extract toxicity