Abstract
Pterodon emarginatus Vogel, Fabaceae, is a great source of bioactive compounds. The most known and studied herbal derivative from this species is an ambar-colored oleoresin that contains vouacapane diterpenes and volatile terpenoids, such as β-caryophyllene. Some recent papers aimed to generate nanoemulsions using this oleoresin for biological applications. However, they used high-energy methods that elevate costs of the process or heating procedures, which offer the disadvantage of possible volatile substances loss. Thus, as part of our ongoing studies with nanobiotechnology of natural products, especially regarding preparation of nanoemulsions with promising plant-based oils by low cost and low energy methods, we decided to evaluate the ability of non-heating and solvent-free method to generate P. emarginatus oleoresin-based nanoemulsions. Two non-ionic surfactants were used to generate the nanoemulsions by a simple homogenization method with vortex stirrer. Low mean droplet size (<180nm) and low polydispersity index (<0.200) were observed even after one day of preparation. The low coefficient of variation for the analyzed parameters of different batches and similar profile for droplet size distribution suggested reproducibility of the method. After 30 days, some degree of droplet growth was observed on nanoemulsion prepared with polyethyleneglycol 400 monooleate, while almost no alteration was observed for nanoemulsion prepared with polysorbate 85. Programmed temperature ramp analysis revealed that no major effects on droplet size and polydispersity index were observed, suggesting the robustness of formed nanoemulsions. Thus, the present study shows for the first time the formation of sucupira-based nanoemulsions by a simple, low cost and ecofriendly method. This study opens new perspectives for bioactive evaluation of this novel nano-product.
Article PDF
References
Baldissera, M.D., da Silva, A.S., Oliveira, C.B., Zimmermann, C.E.P., Vaucher, R.A., Santos, R.C.V., Recti, V.C., Tonin, A.A., Giongo, J.L., Mattos, C.B., Koester, L., Santurio, J.M., Monteiro, S.G., 2013. Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: in vitro tests. Exp. Parasitol. 134, 356–361.
Dias, D.O., Colombo, M., Kelmann, R.G., Souza, T.P., Bassani, V.L., Teixeira, H.F., Veiga Jr., V.F., Limberger, R.P., Koester, L.S., 2012. Optimization of headspace solid-phase microext raction for analysis of β-caryophyllene in a nanoemulsion dosage form prepared with copaiba (Copaifera multijuga Hayne) oil. Anal. Chim. Acta 721, 79–84.
Dias, D.O., Colombo, M., Kelmann, R.G., Kaiser, S., Lucca, L.G., Teixeira, H.F., Limberger, R.P., Veigajr, V.F., Koester, L.S., 2014. Optimization of Copaiba oil-based nanoemulsions obtained by different preparation methods. Ind. Crop. Prod. 59, 154–162.
Duarte, J.L., Amado, J.R.R., Oliveira, A.E.M.F.M., Cruz, R.A.S., Ferreira, A.M., Souto, R.N.P., Falcão, D.Q., Carvalho, J.C.T., Fernandes, C.P., 2015. Evaluation of larvi-cidal activity of a nanoemulsion of Rosmarinus officinalis essential oil. Rev. Bras. Farmacogn. 25, 189–192.
Fernandes, C.P., Mascarenhas, M.P., Zibetti, F.M., Lima, B.C., Oliveira, R.P.R.F., Rocha, L., Falcao, D.Q., 2013. HLB value, an important parameter for the development of essential oil phytopharmaceuticals. Rev. Bras. Farmacogn. 23, 108–114.
Guttoff, M., Saberi, A.H., McClements, D.J., 2015. Formation of vitamin D nanoemulsion-based delivery systems by spontaneous emulsiflcation: factors affecting particle size and stability. Food Chem. 171, 117–122.
Hansen, D., Haraguchi, M., Alonso, A., 2010. Pharmaceutical properties of “sucupira” (Pterodon spp.). Braz. J. Pharm. Sci. 46, 607–616.
Hoscheid, J., Cardoso, M.L.C., 2015. Sucupira as a potential plant for arthritis treatment and other diseases. Arthritis, https://doi.org/10.1155/2015/379459.
Hoscheid, J., Outuki, P.P., Kleinubing, S.A., Silva, M.F., Bruschi, M.X., Cardoso, M.L.C., 2015. Development and characterization of Pterodon pubescens oil nanoemulsions as a possible delivery system for the treatment of rheumatoid arthritis. Colloids Surf. A: Physicochem. Eng. Asp. 484, 19–27.
Komaiko, J., McClements, D.J., 2015. Low-energy formation of edible nanoemulsions by spontaneous emulsiflcation: factors influencing particle size. J. Food Eng. 146, 122–128.
Lima, H.C. de, Lima, L.B. Pterodon in Lista de Espécies da Flora do Brasil. Jardim Botanico do Rio de Janeiro. Available at: http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB29842 (accessed 28.06.16).
Mahajan, J.R., Monteiro, M.B., 1973. New diterpenoids from Pterodon ernarginatus Vog. J. Chem. Soc. 1, 520–525.
Oliveira, A.E.M.F.M., Duarte, J.L., Amado, J.R.R., Cruz, R.A.S., Rocha, C.F., Souto, R.N.P., Ferreira, R.M.A., Santos, K., Conceição, E.C., Oliveira, L.A.R., Kelecom, A., Fernandes, C.P., Carvalho, J.C.T., 2016. Development of alarvicidal nanoemulsion with Pterodon ernarginatus Vogel Oil. PLOS ONE 11, e0145835.
Ostertag, F., Weiss, J., McClements, D.J., 2012. Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. J. Colloid Interface Sci. 388, 95–102.
Pascoa, H., Diniz, D.G.A., Florentino, I.F., Costa, E.A., Bara, M.T.F., 2015. Microemulsion based on Pterodon emarginatus oil and its anti-inflammatory potential. Braz. J. Pharm. Sci. 51, 117–126.
Rodrigues, E.C.R., Ferreira, A.M., Vilhena, J.C.E., Almeida, F.B., Cruz, R.A.S., Florentino, A.C., Souto, R.N.P., Carvalho, J.C.T., Fernandes, C.P., 2014. Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin. Rev. Bras. Farmacogn. 24, 699–705.
Saberi, A.H., Fang, Y., McClements, D.J., 2013a. Effect of glycerol on formation, stability, and properties of vitamin-E enriched nanoemulsions produced using spontaneous emulsification. J. Colloid Interface Sci. 411, 105–113.
Saberi, A.H., Fang, Y., McClements, D.J., 2013b. Fabrication of vitamin E-enriched nanoemulsions by spontaneous emulsification: effect of propylene glycol and ethanol on formation, stability, and properties. Food Res. Int. 54, 812–820.
Saberi, A.H., Fang, Y., McClements, D.J., 2014. Stabilization of vitamin E-enriched mini-emulsions: influence of organic and aqueous phase compositions. Colloids Surf. A: Physicochem. Eng. Asp. 449, 65–73.
Solans, C., Sole, I., 2012. Nanoemulsions: formation by low-energy methods. Curr. Opin. Colloid Interface Sci. 17, 246–254.
Sugumar, S., Ghosh, V., Mukherjee, A., Chandrasekaran, N., 2016. Essential oil-based nanoemulsion formation by low- and high-energy methods and their application in food preservation against food spoilage microorganisms. In Preedy, V.R. (org.) Essential Oils in Food Preservation, Flavor and Safety. Academic Press, pp. 93–100.
Tan, T.B., Yussof, N.S., Abas, F., Mirhosseini, H., Nehdi, I.A., Tan, C.P., 2016. Forming a lutein nanodispersion via solvent displacement method: the effects of processing parameters and emulsifiers with different stabilizing mechanisms. FoodChem. 194, 416–423.
Wang, L., Dong, J., Chen, J., Eastoe, J., Li, X., 2009. Design and optimization of a new self-nanoemulsifying drug delivery system. J. Colloid Interface Sci. 330, 443–448.
Zorzi, G.K., Carvalho, E.L.S., von Poser, G.L., Teixeira, H.F., 2015. On the use of nanotechnology-based strategies for association of complex matrices from plant extracts. Rev. Bras. Farmacogn. 25, 426–436.
Author information
Authors and Affiliations
Corresponding author
Additional information
Authors’ contributions
AEMFMO (PhD student), RASC (PhD student) and JLD (undergraduate student) contributed running the laboratory work, analysis of the data and drafted the paper. ECC contributed to oleoresin extraction, characterization and critical reading of the manuscript. JCTC and CPF designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Oliveira, A.E.M.F.M., Duarte, J.L., Cruz, R.A.S. et al. Utilization of dynamic light scattering to evaluate Pterodon emarginatus oleoresin-based nanoemulsion formation by non-heating and solvent-free method. Rev. Bras. Farmacogn. 27, 401–406 (2017). https://doi.org/10.1016/j.bjp.2016.11.005
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1016/j.bjp.2016.11.005