Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense

Abstract

Aiming to investigate new therapeutic agents with fewer side effects, the number of studies about natural products has increased. Phenolic compounds comprise a well-studied class of abundant plant-derived compounds, whose anti-inflammatory activity has been described. Isoflavones are phenolic compounds that occur mainly in the Leguminosae family, and can be found in many species, such as Trifolium riograndense Burkart, Leguminosae (clover). In this study an HPLC method was used to determine and quantify four isoflavones (genistein, daidzein, formononetin, and biochanin A) in hydrolyzed leaf, flower, stolon, and root extracts of T. riograndense. In vivo anti-inflammatory activity was investigated using the rat paw edema method and in vitro chemotaxis model with a dry extract from the leaves, which had the highest amount of isoflavones. The major isoflavone found in all parts of the plant was formononetin. The chemotaxis assay revealed that the different concentrations (0.2–50 μg/ml) of the dry extract significantly inhibited neutrophil migration in a concentration-dependent manner (more than 90%). In the rat paw edema test, oral administration of clover extract 100 mg/kg was able to significantly inhibit the edema formation induced by carrageenan. In conclusion, chemical analyses showed that Trifolium riograndense is a plant rich in isoflavones and a new interesting option as isoflavone source. The results of the biological tests taken together show that the extract of T. riograndense has anti-inflammatory effect in rodents.

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Correspondence to José A. S. Zuanazzi.

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Author’s contribution

GPRP, CBM, GRD and PCC carried out the phytochemical process. GPRP and GRD made the chromatographic assays. MAA and JASZ contributed in writing the manuscript. MAA, EESS and GRD performed the biological analysis. MDA helped in the collection and identification of the plant material. MAA and EESS contributed to the critical reading the manuscript. All authors have approved the final version for publishing.

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Pedrazza, G.P.R., Morais, C.B., Dettenborn, G.R. et al. Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense. Rev. Bras. Farmacogn. 27, 334–338 (2017). https://doi.org/10.1016/j.bjp.2016.11.004

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Keywords

  • HPLC
  • Isoflavones
  • Leguminosae
  • Neutrophil chemotaxis
  • Rat paw edema