Validated high performance thin layer chromatography method for simultaneous determination of quercetin and gallic acid in Leea indica

Abstract

A sensitive and reliable high performance thin layer chromatography method has been developed for the simultaneous estimation of quercetin and gallic acid in Leea indica, Vitaceae. Ethyl acetate extract prepared from hydrolysed aqueous alcoholic extract (70%) was applied on silica gel G 60 F254 plate. The plate was developed using toluene-ethyl acetate-formic acid, 5:4:1 (v/v/v) as a mobile phase and detection and quantification were performed by densitometric scanning at 254 nm. The system was found to give well resolved bands for quercetin (Rf 0.63) and gallic acid (Rf 0.45) from other constituents present in the extract of L. indica. The correlation coefficient was found to be 0.991 and 0.999 with relative standard deviation, 0.97–1.23% and 0.1–1.13% for quercetin and gallic acid respectively in the developed method. The accuracy of the method was confirmed by conducting recovery studies at different levels using the standard addition method. The average recovery of quercetin and gallic acid was found close to 99% suggesting the accurateness of the method. The proposed validated high performance thin layer chromatographic method offers a new, sensitive, specific and precise gauge for quantification of quercetin and gallic acid in L. indica.

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Correspondence to Mamta B. Shah.

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Authors’ contributions

AAP (M. Pharm. student) contributed in collecting plant sampleand identification, confection of herbarium, running the laboratory work, analysis of the data and drafted the paper. AAA and AHP contributed to chromatographic analysis. AAA contributed to critical reading of the manuscript. MBS designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Patel, A.A., Amin, A.A., Patwari, A.H. et al. Validated high performance thin layer chromatography method for simultaneous determination of quercetin and gallic acid in Leea indica. Rev. Bras. Farmacogn. 27, 50–53 (2017). https://doi.org/10.1016/j.bjp.2016.05.017

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Keywords

  • Gallic acid
  • HPTLC
  • Leea indica
  • Quercetin
  • Simultaneous estimation
  • Validation