Isoflavone-aglycone fraction from Glycine max: a promising raw material for isoflavone-based pharmaceutical or nutraceutical products

Abstract

The present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extract containing isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavone glycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery of the aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. All the extraction and purification parameters were optimized based on the isoflavones yields, which were analyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of the process conditions was accomplished using the classical one-variable-at-a-time method. The identity and purity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis, Fourier transformed-infrared spectroscopy, and 1H and 13C nuclear magnetic resonance spectroscopy. The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning electron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particle size, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96mg/g) as well as the high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material for the production of isoflavone-aglycones based pharmaceuticals or functional foods.

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Acknowledgements

The authors are grateful to the Brazilian government agencies (CAPES, CNPq and FAPERGS - PPSUS projeto 1299-2551/13-0).

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FKJY carried out the main laboratory work as part of her PhD thesis. VLB and LSK are the supervisors who provided intellectual input. All authors participated in the writing of the manuscript and approved its content.

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Correspondence to Valquiria L. Bassani.

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Yatsu, F.K.J., Koester, L.S. & Bassani, V.L. Isoflavone-aglycone fraction from Glycine max: a promising raw material for isoflavone-based pharmaceutical or nutraceutical products. Revista Brasileira de Farmacognosia 26, 259–267 (2016). https://doi.org/10.1016/j.bjp.2015.12.004

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Keywords

  • Isoflavone-aglycone fraction
  • Daidzein
  • Flavonoids
  • Genistein
  • Glycitein
  • Soy