Anti-inflammatory activity and acute toxicity studies of hydroalcoholic extract of Herissantia tiubae


Hydroalcoholic extract of aerial parts of Herissantia tiubae (K. Schum.) Brizicky, Malvaceae, was evaluated in experimental models of inflammation and toxicity. For toxicity assays, male and female Swiss mice were orally treated with hydroalcoholic extract of H. tiubae (2000 mg/kg) and analyzed by consumption of water and food, body weight, mortality and rates of major organ weights, as well as biochemical and hematological indexes. For anti-inflammatory effect, phlogistic agents such as carrageenan or acetic acid were used to evaluate paw edema, cell migration and cytokine production. It was also investigated the hydroalcoholic extract of H. tiubae in RAW 264.7 macrophage lineage by nitric oxide and cytokine productions. Swiss mice treated with hydroalcoholic extract of H. tiubae showed low toxicity and (50 or 100 mg/kg) was able to reduce significantly (p < 0.01, p < 0.001 ) polymorphonuclear cell migration, TNF-a and IL-1ß production in the carrageenan-induced peritonitis. However the hydroalcoholic extract of H. tiubae (50, 100 or 200 mg/kg) did not reduce carrageenan-induced paw edema. Additionally, hydroalcoholic extract of H. tiubae did not present cytotoxicity at concentrations of 6.25, 12.5, 25 or 50 (ig/ml but induced significantly decrease of NO, TNF-a and IL-6 production in macrophage lineage. This study suggests that hydroalcoholic extract of H. tiubae has anti-inflammatory activity by inhibiting cell migration mainly by decreasing the inflammatory cytokine levels at the inflamed site independently of the anti-edematogenic effect.


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This study was financially supported by INCT for Cancer Control, CNPq 573806/2008-0, FAPERJ E26/170.026/2008, CNPq-Universal14/2012-472853/2012-0 and CAPES/Brazil.

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ALAL carried out the study, participated in the toxicological and anti-inflammatory assays and wrote the paper;MFVS provided the plant; WNM has performed the chemical studies; ALX, TMM and MVSCB provided assistance in the acute toxicity; FCL, AFA, TRRO participated in the anti-inflammatory experiments; MRP supervised the work and corrected the manuscript for publication. All the authors have read the final manuscript and approved the submission.

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Correspondence to Marcia R. Piuvezam.

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The authors declare no conflicts of interest.

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Lima, A.L.A., Alves, A.F., Xavier, A.L. et al. Anti-inflammatory activity and acute toxicity studies of hydroalcoholic extract of Herissantia tiubae. Revista Brasileira de Farmacognosia 26, 225–232 (2016).

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  • Malvaceae
  • Herrisantia tiubae
  • Acute inflammation
  • Acute toxicity
  • Anti-inflammatory activity
  • Mice