Anti-inflammatory activity and acute toxicity studies of hydroalcoholic extract of Herissantia tiubae

Abstract

Hydroalcoholic extract of aerial parts of Herissantia tiubae (K. Schum.) Brizicky, Malvaceae, was evaluated in experimental models of inflammation and toxicity. For toxicity assays, male and female Swiss mice were orally treated with hydroalcoholic extract of H. tiubae (2000 mg/kg) and analyzed by consumption of water and food, body weight, mortality and rates of major organ weights, as well as biochemical and hematological indexes. For anti-inflammatory effect, phlogistic agents such as carrageenan or acetic acid were used to evaluate paw edema, cell migration and cytokine production. It was also investigated the hydroalcoholic extract of H. tiubae in RAW 264.7 macrophage lineage by nitric oxide and cytokine productions. Swiss mice treated with hydroalcoholic extract of H. tiubae showed low toxicity and (50 or 100 mg/kg) was able to reduce significantly (p < 0.01, p < 0.001 ) polymorphonuclear cell migration, TNF-a and IL-1ß production in the carrageenan-induced peritonitis. However the hydroalcoholic extract of H. tiubae (50, 100 or 200 mg/kg) did not reduce carrageenan-induced paw edema. Additionally, hydroalcoholic extract of H. tiubae did not present cytotoxicity at concentrations of 6.25, 12.5, 25 or 50 (ig/ml but induced significantly decrease of NO, TNF-a and IL-6 production in macrophage lineage. This study suggests that hydroalcoholic extract of H. tiubae has anti-inflammatory activity by inhibiting cell migration mainly by decreasing the inflammatory cytokine levels at the inflamed site independently of the anti-edematogenic effect.

References

  1. Albuquerque, U.P., Medeiros, P.M., Almeida, A.L.S., 2007. Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: a quantitative approach. J. Ethnopharmacol. 114, 325–354.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Almeida, R.N., Falcão, A.C.G.M., Diniz, R.S.T., Quintans-Júnior, L.J., Polari, R.N., Barbosa-Filho, J.M., Agra, M.F., Duarte, J.C., Ferreira, C.D., Antoniolli, A.R., Araújo, C.C., 1999. Metodologia para avaliacäo de plantas com atividade no Sistema Nervoso Central e alguns dados experimentais. Rev. Bras. Farm. 80, 72–76.

    Google Scholar 

  3. Anderson, G.D., 2008. Gender differences in pharmacological response. Int. Rev. Neurobiol. 83, 1–10.

    Article  PubMed  Google Scholar 

  4. Denizot, F., Lang, R., 1986. Rapid colorimetric assay for cell growth and survival. J. Immunol. Methods 89, 271–277.

    CAS  Article  PubMed  Google Scholar 

  5. De Vasconcelos, D.I., Leite, J.A., Carneiro, L.T., Piuvezam, M.R., 2011. Antiinflammatory and antinociceptive activity of ouabain in mice. Mediat. Inflamm. 2011, 1–11.

    Article  Google Scholar 

  6. Devi, K.P., Malar, D.S., Nabavi, S.F., Sureda, A., Xiao, J., Nabavi, S.M., Daglia, M., 2015. Kaempferol and inflammation: from chemistry to medicine. Pharmacol. Res. 99, 1–10.

    CAS  Article  PubMed  Google Scholar 

  7. Falcáo-Silva, V.S., Silva, D.A, Souza, M.F.V., Siqueira-Junior, J.P., 2009. Modulation of drug resistance in Staphylococcus aureus by a kaempferol glycoside from Heris-santia ttubae (Malvaceae). Phytother. Res. 23, 1367–1370.

    Article  PubMed  Google Scholar 

  8. Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., 1982. Analysis of nitrate, nitrite an [15N] nitrate in biological fluids. Anal. Biochem. 126, 131–138.

    CAS  Article  PubMed  Google Scholar 

  9. Guerra, A.S., Malta, D.J., Laranjeira, LP., Maia, M.B., Colaco, N.C., de Lima, M.C., Caldino, S.L., Pitta, L.R., Goncalves-Silva, T., 2011. Anti-inflammatory and antinociceptive activities of indole-imidazolidine derivatives. Int. Immunopharmacol. 11, 1816–1822.

    CAS  Article  PubMed  Google Scholar 

  10. Hibbs, J.B., Taintor, R.R., Vavrin, Z., Rachlin, E.M., 1988. Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157, 87–94.

    CAS  Article  PubMed  Google Scholar 

  11. Kadioglu, O., Nass, J., Saeed, M.E., Schuler, B., Efferth, T., 2015. Kaempferol is an anti-inflammatory compound with activity towards NF-KB pathway proteins. Anticancer Res. 35, 2645–2650.

    CAS  PubMed  Google Scholar 

  12. Loram, L.C., Fuller, A., Fick, L.G., Cartmell, T., Poole, S., Mitchell, D., 2007. Cytokine profiles during carrageenan-induced inflammatory hyperalgesia in rat muscle and hind paw. J. Pain 8, 127–136.

    CAS  Article  PubMed  Google Scholar 

  13. Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63.

    CAS  Article  Google Scholar 

  14. Oliveira, A.M.F., Pinheiro, L.S., Pereira, C.K.S., Matias, W.N., Gomes, R.A., Chaves, O.S., Souza, M.F.V., Almeida, R.N., Assis, T.S., 2012. Total phenolic content and antioxidant activity of some Malvaceae family species. Antioxidants 1, 33–43.

    Article  Google Scholar 

  15. Paiva, D.C., Dos Santos, CA., Diniz, J.C, Viana, F.A., Thomazzi, S.M., Falcáo, D.A., 2013. Anti-inflammatory and antinociceptive effects of hydroalcoholic extract from Pseudobombax marginatum inner bark from caatinga potiguar. J. Ethnopharmacol. 149, 416–421.

    Article  PubMed  Google Scholar 

  16. Park. J.W., Kwon, O.K., Jang, H.Y., Jeong, H., Oh, S.R., Lee, H.K., Han, S.B., Ahn, K.S., 2012. A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-KB signaling pathway in RAW 264.7 macrophages. Inflammation 35, 321–331.

    Article  PubMed  Google Scholar 

  17. Pinheiro, M.M., Fernandes, S.B., Fingolo, C.E., Boylan, F., Fernandes, P.D., 2013. Anti-inflammatory activity of ethanol extract and fractions from Couroupita guianensis Aublet leaves. J. Ethnopharmacol. 146, 324–330.

    CAS  Article  PubMed  Google Scholar 

  18. Posadas, L., Bucci, M., Roviezzo, F., Rossi, A., Parente, L., Sautebin, L., Cirino, G., 2004. Carrageenan-induced mouse paw oedema is biphasic, age-weight dependent and displays differential nitric oxide cyclooxygenase-2 expression. Br.J. Pharmacol. 142, 331–338.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Schmidt, S., Moser, M., Sperandio, M., 2013. The molecular basis of leukocyte recruit-ment and its deficiencies. Mol. Immunol. 55, 49–58.

    CAS  Article  PubMed  Google Scholar 

  20. Silva, D.A., Falccão-Silva, V.S., Gomes, A.Y.S., Costa, D.A., Lemos, V.S., Agra, M.F., Barz-Filho, R., Siqueira-Junior, J.P., Souza, M.F.V., 2009. Triterpenes and phenolic compounds isolated from the aerial parts of Henssantia tiubae and evaluation of 5, 49-dihydroxy-3, 6, 7, 8, 39-pentamethoxyflavone as a modulator of bacterial drug resistance. Pharm. Biol. 47, 128–133.

    Article  Google Scholar 

  21. Sousa, L.P., Lopes, F., Silva, D.M., Vieira, A.T., Rezende, B.M., Carmo, A.F., Russo, R.C., Garcia, C.C., Bonjardim, CA., Alessandri, A.L., Rossi, A.G., Pinho, V., Teixeira, M.M., 2010. PDE4 inhibition drives resolution of neutrophilic inflammation by inducing apoptosis in a PKA-PBK/Akt-dependent and NF-kappaB-independent manner. J. Leukoc. Biol. 87, 895–904.

    CAS  Article  PubMed  Google Scholar 

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Acknowledgments

This study was financially supported by INCT for Cancer Control, CNPq 573806/2008-0, FAPERJ E26/170.026/2008, CNPq-Universal14/2012-472853/2012-0 and CAPES/Brazil.

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Contributions

ALAL carried out the study, participated in the toxicological and anti-inflammatory assays and wrote the paper;MFVS provided the plant; WNM has performed the chemical studies; ALX, TMM and MVSCB provided assistance in the acute toxicity; FCL, AFA, TRRO participated in the anti-inflammatory experiments; MRP supervised the work and corrected the manuscript for publication. All the authors have read the final manuscript and approved the submission.

Corresponding author

Correspondence to Marcia R. Piuvezam.

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The authors declare no conflicts of interest.

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Lima, A.L.A., Alves, A.F., Xavier, A.L. et al. Anti-inflammatory activity and acute toxicity studies of hydroalcoholic extract of Herissantia tiubae. Revista Brasileira de Farmacognosia 26, 225–232 (2016). https://doi.org/10.1016/j.bjp.2015.11.001

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Keyword

  • Malvaceae
  • Herrisantia tiubae
  • Acute inflammation
  • Acute toxicity
  • Anti-inflammatory activity
  • Mice