Microscopic and UV/Vis spectrophotometric characterization of Cissampelos pareira of Brazil and Africa

Abstract

Cissampelos pareira L., belonging to Menispermaceae family, has worldwide distribution, occurring in tropical and subtropical regions of the Americas, Africa and Asia. It is the most popular species of Cissampelos, known for its medicinal uses of leaves and roots. The study aims to find distinctive leaf anatomical characters, and also demonstrate the importance of spectral data to identify C. pareira samples, in order to contribute to its taxonomy and quality control of its drugs. Anatomical leaf analyses were performed by optical and scanning electron microscopy. The spectral profile was obtained from methanolic extracts of C. pareira samples from Brazil and Africa, with application of UV-vis spectrophotometry data, which were analyzed by principal component analysis (PCA). Some anatomical characters such as leaf epidermal cells walls, stornata, trichomes, mesophyll, features of midrib and petiole, and the spectral profile within the wavelength ranging between 770 and 240 nm (eight bands) differs between Brazilian and African samples. The results represent an additional support to the taxonomy of C. pareira, and the quality control of their leaf drugs, mainly in relation to misidentified samples.

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Acknowledgments

The authors are grateful to CAPES for financial support, and CNPq for the scholarships to NM Porto and MF Agra. The authors thank to Dr. Eduardo de Jesus Oliveira for the English revision and suggestions, and Dr. Rosa Ortiz for her valuable comments and help with the bibliography; to Missouri Botanical Garden for the institutional support; the curators of herbaria CEPEC, IAN, JPB, MG, RB, UFP and MO for their pleasant cooperation during our visits and loan of plant material; and Dulce Gonçalves for her technical support.

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NMP (PhD student) contributed in collecting plant samples and identification, and running the laboratory work (preparing herbaria samples, chemical extraction, obtaining of spectra and plant anatomy studies), analysis of the data and preparation of the paper. YLB contributed to UV analysis, supervised by IJLDB. MFAdesigned the study with Cisampelos pareira, and supervised all laboratory and field work, as well as contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Correspondence to Maria Fátima de Agra.

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Porto, N.M., de Barros, Y.L., Basílio, I.J.L.D. et al. Microscopic and UV/Vis spectrophotometric characterization of Cissampelos pareira of Brazil and Africa. Revista Brasileira de Farmacognosia 26, 135–146 (2016). https://doi.org/10.1016/j.bjp.2015.10.006

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Keywords

  • Ethnomedicine
  • Leaf anatomy
  • Medicinal plant
  • Menispermaceae
  • Morphoanatomy
  • Pharmacobotany