Abstract
Aloe vera (L.) Burm. f., Xanthorrhoeaceae, a succulent, produces barbaloin, a bioactive compounds used in various pharmaceutical products. Extracts prepared from the leaves have been widely used as bittering agents, taste modifiers and also as cathartic agent against severe constipation. Barbaloin is reported for its anti-inflammatory, anticancer, antiviral and anticancer activities and these properties are mostly mediated by its antioxidative capacity. Presently, a study has been conducted on the comparative High Performance Thin Layer Chromatography analysis of barbaloin from the dried leaf skin powder of in vivo and in vitro grown A vera. Shoot tips of A vera were cultured in Murashige and Skoog media supplemented with different combination of 6-benzylaminopurine and 1-naphthaleneacetic acid. [Best multiplication response was noted in benzylaminopurine (2.0 mg/l) +1 -naphthaleneacetic acid (0.1 mg/l) supplemented Murashige and Skoog media]. The quantitative determination of barbaloin was performed on silica gel 60 F254 HPTLC plates as stationary phase. The linear ascending development was carried out in a twin trough glass chamber saturated with a mobile phase consisting of ethyl acetate: methanol: water ( 100:16.5:13.5) at room temperature (22±2°C). CAMAG Thin Layer Chromatography scanner-3 equipped with CATS software (version: 1.4.4.6337) was used for spectrodensitometric scanning and analysis in the ultraviolet region at X = 366 nm. The method was validated for linearity, precision and accuracy. Correlation coefficient, limit of detection, limit of quantification as well as recovery values were found to be satisfactory. Out of the five populations studied, the leaf skin of A vera collected from Jodhpur (Rajasthan, India) and raised in vitro was found to contain higher amount of barbaloin (2.78%) when compared to its naturally growing counterparts (2.46%) and other plant populations.
Article PDF
Similar content being viewed by others
References
Abrie, A.I., Staden, J.V., 2001. Micropropagation of the endangered Aloe polyphylla. Plant Growth Regul. 33, 19–23.
Aggarwal, D., Barna, ICS., 2004. Tissue culture propagation of elite plant of Aloe vera L. J. Plant Biochem. Biotechnol. 13, 77–79.
Aremu, A.O., Gruz, J., Šubrtová, M., Szüčová, L., Doležal, K., Bairu, M.W., et al., 2013. Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins. J. Plant Physiol. 170, 1303–1308.
Bai, Y., Qu, R., 2001. Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue. Plant Breed. 120, 239–242.
Chang, X.L., Wang, C., Feng, Y., Liu, Z., 2006. Effects of heat treatments on the stabilities of polysaccharides substances and barbaloin in gel juice from Aloe vera Miller. J. Food Eng. 75, 245–251.
Chaudhuri, S., Mukundan, U., 2001. Aloe vera L. micropropagation and characterization of its gel. Phytomorphology 51, 155–157.
Chen, J.D., Li, W., Li, S.Y., 2002. Quantitative determination of barbaloin in aloe capsule by high performance liquid chromatography. Se Pu 20, 367–368.
Coran, S.A., Bartolucci, G., Bambagiotti-Alberti, M., 2011. Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode. J. Pharm. Biomed. Anal. 54, 422–425.
Debiasi, C., Silva, C.G., Pescador, R., 2007. Micropropagation of Aloe vera L. Rev. Bras. Plant Med. Botucatu 9, 36–43.
Debnath, M., Malik, C.P., Bisen, P.S., 2006. Micropropagation: a tool forthe production of high quality plant-based medicines. Curr. Pharm. Biotechnol. 7, 33–49.
Gonçalves, S., Romano, A., 2013. In vitro culture of lavenders (Lavandula spp.) and the production of secondary metabolites. Biotechnol. Adv. 31, 166–174.
Groom, Q.J., Reynolds, T., 1987. Barbaloin in Aloe species. Planta Med. 53, 345–348.
Gutterman, Y., Chauser-Volfson, E., 2000. The distribution of the phenolic metabolites barbaloin, aloeresin and aloenin as a peripheral defense strategy in the succulent leaf parts of Aloe arborescens. Biochem. Syst. Ecol. 28, 825–838.
Hamman, J.H., 2008. Composition and applications of Aloe vera leaf gel. Molecules 13, 1599–1616.
Hashemi, S.A., Madani, S.A., Abediankenari, S., 2015. The review on properties of Aloe vera in healing of cutaneous wounds. Biomed. Res. Int. 2015, 714216.
Kantia, A., Kothari, S.L., 2002. High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L. Sci. Hortic. 96, 205–212.
Klein, A.D., Penneys, N.S., 1988. Aloe vera. J. Am. Acad. Dermatol. 18, 714–720.
Mangaiyarkarasi, S.P., Manigandan, T., Elumalai, M., Cholan, P.K., Kaur, R.P., 2015. Benefits of Aloe vera in dentistry. J. Pharm. Bioallied. Sci. 7, S255–S259.
Meyer, H.J., Staden, J.V., 1991. Rapid in vitro propagation of Aloe barbadensis Mill. Plant Cell Tissue Organ. Cult. 26, 167–171.
Molazem, Z., Mohseni, F., Younesi, M., Keshavarzi, S., 2014. Aloe vera gel and cesarean wound healing; a randomized controlled clinical trial. Glob. J. Health Sci. 7, 203–209.
Natali, L., Sanchez, I.C., Cavallini, A., 1990. In vitro culture of Aloe barbadensis Mill.: micropropagation from vegetative meristems. Plant Cell Tissue Organ. Cult. 20, 71–74.
Ngo, M.Q., Nguyen, N.N., Shah, S.A., 2010. Oral Aloe vera for treatment of diabetes mellitus and dyslipidemia. Am. J. Health Syst. Pharm. 67, 1806–1808.
Pandey, D.K., Malik, T., Banik, R.M., 2012. Quantitative estimation of barbaloin in Aloe vera and its commercial formulations by using HPTLC. Int. J. Med. Aromatic Plants 2, 420–427.
Park, C.H., Nam, D.Y., Son, H.U., Lee, S.R., Lee, HJ., Heo, J.C., et al., 2011. Polymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesions. Int. J. Mol. Med. 27, 511–518.
Patel, D.K., Patel, K., Tahilyani, V., 2012. Barbaloin: A concise report of its pharmacological and analytical aspects. Asian Pac. J. Trop. Biomed. 2, 835–838.
Rebecca, W., Kayser, O., Hagels, H., Zessin, K.H., Madundo, M., Gamba, N., 2003. The phytochemical profile and identification of main phenolic compounds from the leaf exudate of Aloe secundiflora by high-performance liquid chromatographymass spectroscopy. Phytochem. Anal. 14, 83–86.
Reynolds, T., Dweck, A.C., 1999. Aloe vera leaf gel: a review update. J. Ethnopharmacol. 68, 3–37.
Roy, S.C., Sarkar, A., 1991. In vitro regeneration and micropropagation of Aloe vera L. Sci. Hortic. 47, 107–113.
Saeed, M.A., Ahmad, I., Yaqub, U., Akbar, S., Waheed, A., Saleem, M., et al., 2004. Aloe vera: a plant of vital significance. Q. Sci. Vis. 9, 1–13.
Sato, F., 2013. Characterization of plant functions using cultured plant cells, and biotechnological applications. Biosc. Biotechnol. Biochem. 77, 1–9.
Surjushe, A., Vasani, R., Saple, D.G., 2008. Aloe vera: a short review. Ind. J. Dermatol. 53, 163–166.
Tanaka, M., Misawa, E., Yamauchi, K., Abe, F., Ishizaki, C., 2015. Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women. Clin. Cosmet. Investig. Dermatol. 20, 95–104.
Velcheva, M., Faltin, Z., Vardi, A., Eshdat, Y., Perl, A., 2005. Regeneration of Aloe arborescens via somatic organogenesis from young inflorescences. Plant Cell Tissue Organ. Cult. 83, 293–301.
Wagner, H., Bladt, S., 1996. Plant drug analysis: a thin layer chromatography atlas. Springer Science & Business Media.
Yonehara, A., Tanaka, Y., Kulkeaw, K., Era, T., Nakanishi, Y., Sugiyama, D., 2015. Aloe vera extract suppresses proliferation of neuroblastoma cells in vitro. Anticancer Res. 35, 4479–4485.
Zahn, M., Trinh, T., Jeong, M.X., Wang, D., Abeysinghe, P., Jia, Q, et al., 2008. A reversed-phase high-performance liquid chromatographic method for the determination of aloesin, aloeresin A and anthraquinone in Aloe ferox. Phytochem. Anal. 19, 122–126.
Zhang, F.L., Takahata, Y., Xu, J.B., 1998. Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep. 17, 780–786.
Acknowledgements
The author is thankful to the Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi for providing HPTLC facility for analysis of the plant materials.
Author information
Authors and Affiliations
Contributions
DKP monitored the experiments and prepared the manuscript draft. SP performed the experiments. AD designed the experimentation and finalized the manuscript.
Corresponding author
Ethics declarations
The authors declare no conflicts of interest.
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Pandey, D.K., Parida, S. & Dey, A. Comparative HPTLC analysis of bioactive marker barbaloin from in vitro and naturally grown Aloe vera. Revista Brasileira de Farmacognosia 26, 161–167 (2016). https://doi.org/10.1016/j.bjp.2015.08.016
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1016/j.bjp.2015.08.016