Evaluation of the orofacial antinociceptive profile of the ethyl acetate fraction and its major constituent, rosmarinic acid, from the leaves of Hyptis pectinata on rodents

Abstract

Hyptis pectinata (L.) Poit, Lamiaceae, popularly known as “sambacaitá, ” is an aromatic shrub largely grown in the Brazilian northeastern. We investigated the antinociceptive effects of the ethyl acetate fraction obtained from the leaves of H. pectinata and of its main constituent rosmarinic acid, on formalin (2%)-, glutamate (25 μM)- and capsaicin (2.5 (μg)-induced orofacial nociception in rodents. Male mice were pretreated with ethyl acetate fraction (100, 200 or 400mg/kg, p.o.), rosmarinic acid (10 or 20mg/kg, p.o.), morphine (5mg/kg, i.p.), or vehicle (distilled water + 0.2% Tween 80). Ethyl acetate fraction reduced the nociceptive face-rubbing behavior during the two phase of the formalin test, whereas pretreatment with rosmarinic acid decreased the pain behavior in the second phase. Ethyl acetate fraction produced significant antinociceptive effects in the capsaicin and glutamate tests. This study showed that oral administration of ethyl acetate fraction produced potent antinociceptive effects compared to treatment with rosmarinic acid.

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Acknowledgements

We would like to thank the CNPq/Brazil — grant number 555076/2010-5 and FACEPE (grant number PRONEM APQ-1232.1.06/10) for financial support.

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Contributions

REAF, SAS and CAC contributed in collecting plant sample and identification, confection of her barium, running the laboratory work, analysis of the data and drafted the paper. JSSQ, PLS, and AANL AANL contributed to biological studies. REAF, SAS and CAC contributed in plant identification, her barium confection and contributed to chromatographic analysis. JSSQ, LJQJ, MTSC, TMSS contributed to critical reading of the manuscript. AGG and LJQJ designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

Corresponding author

Correspondence to Adriana G. Guimarães.

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Falcão, R.E.A., de Souza, S.A., Camara, C.A. et al. Evaluation of the orofacial antinociceptive profile of the ethyl acetate fraction and its major constituent, rosmarinic acid, from the leaves of Hyptis pectinata on rodents. Revista Brasileira de Farmacognosia 26, 203–208 (2016). https://doi.org/10.1016/j.bjp.2015.07.029

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Keywords

  • Hyptis pectinate
  • Rosmarinic acid
  • Orofacial pain
  • Nociception