Preformulation study and influence of DMSO and propylene glycol on the antioxidant action of isocoumarin paepalantine isolated from Paepalanthus bromelioides

Abstract

Coumarins are phenolic compounds and have various biological properties, including antioxidant activity. The isocoumarin paepalantine, isolated from of Paepalanthus bromelioides Silveira, Eriocaulaceae, exhibits a wide range of biological activities, including antimicrobial, anti-inflammatory, antioxidant and cytotoxic properties. Studies on paepalantine often use dimethylsulfoxide as a solvent. However the dimethylsulfoxide interferes with antimicrobial, cytotoxic and antioxidant assays. Thus, this study aims to evaluate alternative solvents for paepalantine and evaluate their potential to interfere with antioxidant assays (ABTS•+, O2•−, HOCl). Of the selected solvents, propylene glycol had good solubility and remained stable throughout the study period. The results suggested that there is no interference from propylene glycol in antioxidant assays, while dimethylsulfoxide significantly interfered with the HOCl assay. The antioxidant assays showed that paepalantine demonstrated similar or even better antioxidant activity than Trolox. Thus, propylene glycol may be the solvent of choice for paepalantine, a compound that has significant biological potential.

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Acknowledgements

This study was supported by a grant of FAPES to J.P.L.D., and financial aid of FAPES to R.R.K. Thanks to Laboratory of Biomolecular Analysis (LABIOM) at Health Sciences Center from Federal University of Espírito Santo, Vitória-ES, Brazil for their support with the use of spectrophotometer UV/VIS NANO DROP.

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JPLD contributed to running the laboratory work for isolation and identification of the paepalantine, biological studies, analysis of the data, and drafting the paper. RRK designed the study, supervised the laboratory work for the isolation and identification of the paepalantine and antioxidant test, and contributed to critical reading of the manuscript. CS supervised the laboratory work in the preformulation studies and contributed to critical reading of the manuscript. RCRG supervised the laboratory work in antioxidant test and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.

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Correspondence to Rodrigo Rezende Kitagawa.

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Damasceno, J.P.L., dos Santos Giuberti, C., de Cássia Ribeiro Gonçalves, R. et al. Preformulation study and influence of DMSO and propylene glycol on the antioxidant action of isocoumarin paepalantine isolated from Paepalanthus bromelioides. Rev. Bras. Farmacogn. 25, 395–400 (2015). https://doi.org/10.1016/j.bjp.2015.07.008

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Keywords

  • Antioxidant
  • Paepalantine
  • Preformulation