Screening of flavonoids rich fractions of three Indian medicinal plants used for the management of liver diseases

Abstract

The decoctions of the Butea monosperma (Lam.) Taub., Fabaceae, Bauhinia variegata L., Fabaceae, and Ocimum gratissimum L., Lamiaceae, are traditionally used for the treatment of various types of hepatic disorder. Phytochemical studies have shown that total flavonoids from these plants were the major constituents of the picked out part of each plant. The present study was planned to investigate the hep-atoprotective effect of flavonoid rich fractions of the B. monosperma, B. variegata and O. gratissimum against paracetamol induced liver damage. Flavonoid rich fractions were isolated by solvent fractionation from each plant. Each fraction was subjected to various qualitative chemical tests to findout the metabolites. Flavonoid fractions of each plant were subjected for pharmacological screening. The rats were monitored for change in liver morphology, biochemical parameters like serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, alkaline phosphatase and total bilirubin for the groups receiving the flavonoid-rich fractions. All flavonoid rich fractions showed significant hep-atoprotective activity. The histological studies supported the biochemical parameters. From the results of biochemical analysis and histopathological studies, it can be accomplished that in the ethyl acetate fraction of 0. gratissimum showed highest hepatoprotective activity as compared to other fractions. The present study was the first evidence of flavonoid-rich fractions of each plant have a remarkable hepatoprotective effect. All fractions contain a potent hepatoprotective agent suggested to be a flavone, which may find clinical application in amelioration of paracetamol-induced liver damage.

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Acknowledgements

The authors express their sincere thanks to Dr. Bhavin Vyas and Janki Desai from Department of Pharmacology for their help.

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The AG was responsible for the plant collections, performed the experiments, analyzed the data and wrote the report. JSY and SP supervised the entire work, analyzed the data and did a critical reading of the manuscript. SVJ helped in biological studies.

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Correspondence to Arti Gupta.

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Gupta, A., Sheth, N.R., Pandey, S. et al. Screening of flavonoids rich fractions of three Indian medicinal plants used for the management of liver diseases. Rev. Bras. Farmacogn. 25, 485–490 (2015). https://doi.org/10.1016/j.bjp.2015.06.010

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Keywords

  • Paracetamol
  • Flavonoid-rich fractions
  • Butea monosperma
  • Bauhinia variegata
  • Ocimum gratissimum
  • Hepatoprotective activity