The decoctions of the Butea monosperma (Lam.) Taub., Fabaceae, Bauhinia variegata L., Fabaceae, and Ocimum gratissimum L., Lamiaceae, are traditionally used for the treatment of various types of hepatic disorder. Phytochemical studies have shown that total flavonoids from these plants were the major constituents of the picked out part of each plant. The present study was planned to investigate the hep-atoprotective effect of flavonoid rich fractions of the B. monosperma, B. variegata and O. gratissimum against paracetamol induced liver damage. Flavonoid rich fractions were isolated by solvent fractionation from each plant. Each fraction was subjected to various qualitative chemical tests to findout the metabolites. Flavonoid fractions of each plant were subjected for pharmacological screening. The rats were monitored for change in liver morphology, biochemical parameters like serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, alkaline phosphatase and total bilirubin for the groups receiving the flavonoid-rich fractions. All flavonoid rich fractions showed significant hep-atoprotective activity. The histological studies supported the biochemical parameters. From the results of biochemical analysis and histopathological studies, it can be accomplished that in the ethyl acetate fraction of 0. gratissimum showed highest hepatoprotective activity as compared to other fractions. The present study was the first evidence of flavonoid-rich fractions of each plant have a remarkable hepatoprotective effect. All fractions contain a potent hepatoprotective agent suggested to be a flavone, which may find clinical application in amelioration of paracetamol-induced liver damage.
Akah, P.A., Odo, C.I., 2010. Hepatoprotective effect of the solvent fractions of the stem of Hoslundia opposita Vahl (Lamiaceae) against carbon tetrachloride- and paracetamol-induced liver damage in rats. Int. J. Green Pharm. 4, 54–58.
Baek, N., Kim, Y., Kyung, J., Park, K., 1996. Isolation of anti-hepatotoxic agent from the root of Astragalus membranaceas. Korean J. Pharmacogn. 27, 111–116.
Balamurugan, G., Muralidharan, P., 2010. Antiobesity effect of Bauhinia variegata L. bark extract on female rats fed on hypercaloric diet. Bangladesh J. Pharmacol. 5, 8–12.
Baranisrinivasan, P., Elumalai, E.K., Sivakumar, C., ViviyanTherasa, S., David, E., 2009. Hepatoprotective effect of Enicostemma littorale blume and Eclipta albaduring ethanol induced oxidative stress in albino rats. Int. J. Pharmacol. 5, 268–272.
Bodakhe, S.H., Ram, A., 2007. Hepatoprotective properties oí Bauhinia variegata L. bark extract. Yakugaku zasshi 127, 1503–1507.
Chattopadhyay, R., 2003. Possible mechanism of hepatoprotective activity of Azadirachta indica eaf extract: Part II. J. Ethnopharmacol. 89, 217–219.
Chiu, C.-C., Huang, C.Y., Chen, T.Y., Kao, S.H., Liu, J.Y., Wang, Y.W., Tzang, B.-S., Hsu, T.-C., 2012. Beneficial effects of Ocimum gratissimum aqueous extract on rats with CC1 4-induced acute liver injury. Evid.-Based Complement. Altern. Med., https://doi.org/10.1155/2012/736752.
Dong, H., Haining, R.L., Thummel, K.E., Rettie, A.E., Nelson, S.D., 2000. Involvement of human cytochrome P450 2D6 in the bioactivation of acetaminophen. Drug Metab. Dispos. 28, 1397–1400.
Grayer, R.J., Kite, G.C., Abou-Zaid, M., Archer, L.J., 2000. The application of atmospheric pressure chemical ionisation liquid chromatography-mass spectrometry in the chemotaxonomic study of flavonoids: characterisation of flavonoids from Ocimum gratissimum var. gratissimum. Phytochem. Anal. 11, 257–267.
Gupta, A., Sheth, N.R., Pandey, S., Shah, D.R., Yadav, J.S., 2013a. Design and evaluation of herbal hepatoprotective formulation against paracetamol induced liver toxicity. J. Young Pharm. 5, 180–187.
Gupta, A., Sheth, N.R., Pandey, S., Shah, D.R., Yadav, J.S., 2013b. Determination of ursolic acid in fractionated leaf extracts of Ocimum gratissimum L. and in developed herbal hepatoprotective tablet by HPTLC. Pharmacogn. J. 5, 156–162.
Gupta, S., Prakash, J., Srivastava, S., 2002. Validation of traditional claim of Tulsi, Ocimum sanctum Linn, as a medicinal plant. Indian J. Exp. Biol. 40, 765–773.
Ighodaro, O., Ebuehi, O., 2008. Aqueous leaf extract of Ocimum gratissimum potentiates activities of plasma and hepatic antioxidant enzymes in rats. Niger. Q. J. Hosp. Med. 19, 106–109.
Janbaz, K., Saeed, S., Gilani, A., 2004. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine 11, 424–430.
Karthikeyan, K., Ravichandran, P., Govindasamy, S., 1999. Chemopreventive effect of Ocimum sanctum on DMBA-induced hamster buccal pouch carcinogenesis. Oral Oncol. 35, 112–119.
Khandelwal, K., 2001. Pharmacognosy: techniques and experiments. Nirali Prakashan 8, 3–40, 146-161.
Kirtikar, K., Basu, B., Dun, D., Singh, B., Singh, M., 1999. Indian medicinal plants Dehradun 1964–1965.
Kosalec, I., Bakmaz, M., Pepeljnjak, S., Vladimir-Knezevic, S., 2004. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm. Zagreb 54, 65–72.
Lee, M.J., Chen, H.M., Tzang, B.S., Lin, C.W., Wang, C.J., Liu, J.Y., Kao, S.H., 2010. Ocimum gratissimum aqueous extract protects H9c2 myocardiac cells from H2O2 -induced cell apoptosis through Akt signalling. Evid.-Based Complement. Altera, https://doi.org/10.1155/2011/578060.
Macdonald, I.O., Oludare, A.S., Olabiyi, A., 2010. Phytotoxic and antimicrobial activities of flavonoids in Ocimum gratissimum. Life Sci. J. 7, 3.
Maldonado, P.D., Barrera, D., Rivero, I., Mata, R., Medina-Campos, O.N., Hernández-Pando, R., Pedraza-Chaverrí, J., 2003. Antioxidant S-allylcysteine prevents gentamicin-induced oxidative stress and renal damage. Free Rad. Biol. Med. 35, 317–324.
Mehta, R., Shankar, M., Geetha, M., Saluja, A., 1999. Hepatoprotective activity of Trianthema portulacastrum. Indian Drugs 36, 241–244.
Muralidhar, A., Babu, K.S., Sankar, T.R., Reddanna, P., Latha, J., 2011. Evaluation of wound healing properties of bioactive fractions from the extract of Butea monosperma (Lam.) Taub (lam) stem bark. Int. J. Phytomed. 3, 41–49.
Nadkarni, K.M., 1994. Dr. KM Nadkarni’s Indian materia medica. 1. Popular Prakashan.
Okoli, C., Ezike, A., Agwagah, O., Akah, P., 2010. Anticonvulsant and anxiolytic evaluation of leaf extracts of Ocimum gratissimum, a culinary herb. Pharmacogn. Res. 2, 36–40.
Onajobi, F.D., 1986. Smooth muscle contracting lipid-soluble principles in chromatographic fractions of Ocimum gratissimum. J. Ethnopharmacol. 18, 3–11.
Pandit, S., Sur, T., Jana, U., Debnath, P., Sen, S., Bhattacharyya, D., 2004. Prevention of carbon tetrachloride-induced hepatotoxicity in rats by Adhatoda vasica leaves. Indian J. Pharmacol. 36, 312–313.
Parasuraman, S., Raveendran, R., Kesavan, R., 2010. Blood sample collection in small laboratory animals. J. Pharmacol. Pharmacother. 1, 87–93.
Prakash, J., Gupta, S., 2000. Chemopreventive activity of Ocimum sanctum seed oil. J. Ethnopharmacol. 72, 29–34.
Prashar, R., Kumar, A., Banerjee, S., Rao, A., 1994. Chemopreventive action by an extract from Ocimum sanction on mouse skin papillomagenesis and its enhancement of skin glutathione S-transferase activity and acid soluble sulfydryl level. Anticancer Drugs 5, 567–572.
Puratchikody, A., Devi, C.N., Nagalakshmi, G., 2006. Wound healing activity of cype-rus rotundus linn. Indian J. Pharm. Sci. 68, 97–101.
Raju, R.W., Radhika, S.S., Kunal, M.T., Kalpana, S.P., Sunil, S.J., 2008. Screening of roots of Baliospermum montanum for hepatoprotective activity against paracetamol induced liver damage in albino rats. Int. J. Green Pharm. 2, 220–223.
Ramachandran, K., Kashyapa, K., Chand, R., 1986. The Useful Plants of India. Council of Scientific and Industrial Research, New Delhi, pp. 671–679.
Sharma, A., Chakraborti, K., Handa, S., 1991. Antihepatotoxic activity of some Indian herbal formulations as compared to Silymarin. Fitoterapia 62, 229–235.
Sharma, A.K., Deshwal, N., 2011. An overview: on phytochemical and pharmacological studies of Butea monosperma. Int. J. Pharm. Tech. Res. 3, 864–871.
Singab, A.N.B., Youssef, D.T., Noaman, E., Kotb, S., 2005. Hepatoprotective effect of flavonol glycosides rich fraction from Egyptian Vicia calcarata desf. against CCI4-induced liver damage in rats. Arch. Pharm. Res. 28, 791–798.
Silva, M.G., Vieira, I.G., Mendes, F.N., Albuquerque, I.L., dos Santos, R.N., Silva, F.O., Morais, S.M., 2008. Variation of ursolic acid content in eight Ocimum species from northeastern Brazil. Molecules 13, 2482–2487.
Yuan, L.P., Chen, F.H., Ling, L., Dou, P.F., Bo, H., Zhong, M.M., Xia, L.J., 2008. Protective effects of total flavonoids of Bidens pilosa L. (TFB) on animal liver injury and liver fibrosis. J. Ethnopharmacol. 116, 539–546.
Yuvaraj, P., Louis, T., Madhavachadran, V., Gopinath, N., Rekha, S., 2011. Total phenolic content and screening of antioxidant activity of selected ayurvedic medicinal plants. Ayurvedic Renaiss. 91, 25–31.
Varley, H., Van, E., Kass, I., 1994. Practical clinical chemistry. William Heinemann Med. Books London 1, 891–907.
Xiong, X., Chen, W., Cui, J., Yi, S., Zhang, Z., Li, K., 2003. Zhong yao cai Zhongyaocai (Effects of ursolic acid on liver-protection and bile secretion). J. Chin. Med. Mater. 26, 578–581.
Zhong, M.M., Chen, F.H., Yuan, L.P., Wang, X.H., Wu, F.R., Yuan, F.L, Cheng, W.M., 2007. Protective effect of total flavonoids from Bidens bipinnata L against carbon tetrachloride-induced liver injury in mice. J. Pharm. Pharmacol. 59, 1017–1025.
The authors express their sincere thanks to Dr. Bhavin Vyas and Janki Desai from Department of Pharmacology for their help.
The authors declare no conflicts of interest.
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Gupta, A., Sheth, N.R., Pandey, S. et al. Screening of flavonoids rich fractions of three Indian medicinal plants used for the management of liver diseases. Rev. Bras. Farmacogn. 25, 485–490 (2015). https://doi.org/10.1016/j.bjp.2015.06.010
- Flavonoid-rich fractions
- Butea monosperma
- Bauhinia variegata
- Ocimum gratissimum
- Hepatoprotective activity