Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis

Abstract

The aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 × 4.6 mm, 5 μm). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.

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Acknowledgements

This study was supported by Fapesq, Propesq/UEPB, Capes and CNPq (Process No.: 562957/2010-3), Brazil for scholarships and research fellowships. The authors especially wish to give thanks to Professor Ivan Coelho Dantas (in memoriam) for indicating the variety of plant to be studied in this work.

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Fernandes, F.H.A., de Batist, R.S.A., de Medeirosa, F.D. et al. Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis. Rev. Bras. Farmacogn. 25, 208–211 (2015). https://doi.org/10.1016/j.bjp.2015.05.006

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Keywords

  • Gallic acid
  • Chemical marker
  • Validation
  • Schinopsis brasiliensis