Abstract
Arrabidaea brachypoda Bureau, Bignoniaceae, known as “cipó-una”, is widely used in traditional medicine in Southeastern and Northeastern Brazil for kidney stones and painful joints. This study was aimed at evaluating the anti-inflammatory proprieties of the oleanane-type triterpenoid 3β-estearioxy-olean-12-ene isolated from the roots of A. brachypoda. Carrageenan-induced paw oedema, formalin test and hot plate test were used to investigate the antiinflammatory activity of 3β-estearioxy-olean-12-ene in animals. We observed that 3β-estearioxy-olean-12-ene at doses of 5, 10 and 15 mg/kg p.o. demonstrated anti-inflammatory effects, by reduced (p < 0.05) paw oedema induced by carrageenan and by decreased (p < 0.05) licking time caused by a subplantar injection of formalin. In conclusion, 3β-estearioxy-olean-12-ene, a triterpene isolated from the roots of A. brachypoda, demonstrate anti-inflammatory effect in different tests. Thus, it may be useful in the treatment of inflammatory disorders, which supports previous claims of its traditional use.
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Abbott, F.V., Franklin, K.B., Westbrook, R.F., 1995. The formalin test: scoring properties of the first and second phase of the pain response in rats. Pain 60, 91–102.
Agra, M.D., Silva, K.N., Basílio, I.J., de Freitas, P.F., Barbosa-Filho, J.M., 2008. Survey of medicinal plants used in the region Northeast of Brazil. Rev. Bras. Farmacogn. 18, 472–508.
Alcerito, T., Barbo, F.E., Negri, G., Santos, D.Y., Meda, C.I., Young, M.C., Chavez, D., Blatt, C.T., 2002. Foliar epicuticular wax of Arrabidaea brachypoda: flavonoids and antifungal activity. Biochem. Syst. Ecol. 30, 677–683.
Blatt, C.T., dos Santos, M.D., Salatino, A., 1998. Flavonoids of Bignoniaceae from the “cerrado” and their possible taxonomic significance. Plant Syst. Evol. 210, 289–292.
Bolzani, V.S., Pauleti, P.M., Young, M.C., 2003. Chemical constituents of Arrabidaea samydoides (Bignoniaceae). Quim. Nova 26, 641–643.
Brito, A.R.M.S., Antonio, M.A., 1998. Oral anti-inflammatory and antiulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turneraceae). J. Ethnopharmacol. 61, 215–228.
da Rocha, C.Q., Queiroz, E.F., Meira, C.S., Moreira, D.R., Soares, M.B., Marcourt, L., Vilegas, W., Wolfender, J.L., 2014. Dimeric flavonoids from Arrabidaea brachypoda and assessment of their anti-Trypanosoma cruzi activity. J. Nat. Prod. 77, 1345–1350.
da Rocha, C.Q., Vilela, F.C., Cavalcante, G.P., Santa-Cecília, F.V., Santos-e-Silva, L., dos Santos, M.H., Giusti-Paiva, A., 2011. Anti-inflammatory and antinociceptive effects of Arrabidaea brachypoda (DC.) Bureau roots. J. Ethnopharmacol. 133, 396–401.
de Medeiros, P.M., Ladio, A.H., Albuquerque, U.P., 2013. Patterns of medicinal plant use by inhabitants of Brazilian urban and rural areas: a macroscale investigation based on available literature. J. Ethnopharmacol. 150, 729–746.
Davidson, E.M., Carlton, S.M., 1998. Intraplantar injection of dextrorphan, ketamine or mematine attenuates formalin-induced behaviors. Brain Res. 785, 136–142.
Leite, J.P., Oliveira, A.B., Lombardi, J.A., Souza-Filho Chiari, J.D., 2006. Trypanocidal activity of triterpenes from Arrabidaea triplinervia and derivatives. Biol. Pharm. Bull. 29, 2307–2309.
Martin, F., Hay, A.E., Cressend, D., Reist, M., Vivas, L., Gupta, M.P., Carrupt, P.A., Hostettmann, K., 2008. Antioxidant C-glucosylxanthones from the leaves of Arrabidaea patellifera. J. Nat. Prod. 71, 1887–1890.
Mendes, S.S., Bomfim, R.R., Jesus, H.C.R., Alves, P.B., Blank, A.F., Estevam, C.S., Antoniolli, A.R., Thomazzi, S.M., 2010. Evaluation of the analgesic and anti-inflammatory effects of the essential oil of Lippia gracilis leaves. J. Ethnopharmacol. 129, 391–397.
Mendonça, R.C., Felfili, J.M., Walter, B.M., Silva, M.C., Rezende, A.R., Filguieras, T.S., Nogueira, P.E., 1998. Flora vascular do Cerrado. In: Sano, S.M., Almeida, S.P. (Eds.), Cerrado ambiente e flora. EMBRAPA, Planaltina Distrito Federal, pp. 286–556.
Recio, M.C., Giner, R.M., Má˜nez, S., Ríos, J.L., 1995. Structural requirements for the anti-inflammatory activity of natural triterpenoids. Planta Med. 61, 182–185.
Rodrigues, E., Mendes, F.R., Negri, G., 2006. Plants indicated by Brazilian Indians to Central Nervous System disturbances: a bibliographical approach. Curr. Med. Chem.: Cent. Nerv. Syst. Agents 6, 211–244.
Safayhi, H., Sailer, E.R., 1997. Anti-inflammatory actions of pentacyclic triterpenes. Planta Med. 63, 487–493.
Santos, A.R., Calixto, J.B., 1997. Further evidence for the involvement of tachykinin receptor subtypes in formalin and capsaicin models of pain in mice. Neuropeptides 31, 381–389.
Seibert, K., Zhang, Y., Leahy, K., Hauser, S., Masferrer, J., Perkins, W., Lee, L., Isakson, P., 1994. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc. Natl. Acad. Sci. U.S.A. 91, 12013–12017.
Singh, G.B., Singh, S., Bani, S., Gupta, B.D., Banerjee, S.K., 1992. Anti-inflammatory activity of oleanolic acid in rats and mice. J. Pharm. Pharmacol. 44, 456–458.
Thomazzi, S.M., Silva, C.B., Silveira, D.C.R., Vasconcellos, C.L.C., Lira, A.F., Cambui, E.V.F., Estevam, C.S., Antoniolli, A.R., 2010. Antinociceptive and antiinflammatory activities of Bowdichia virgilioides (sucupira). J. Ethnopharmacol. 127, 451–456.
Vieira-Filho, S.A., Duarte, L.P., Silva, G.D.F., Howart, O.W., Lula, I.S., 2003. 3-(Stearyloxy)olean-12-ene from Austroplenckia populnea: structure elucidation by 2D-NMR and quantitative 13C NMR spectroscopy. Helv. Chim. Acta 86, 3445–3449.
Vilela, F.C., Bitencourt, A.D., Cabral, L.D., Franqui, L.S., Soncini, R., Giusti-Paiva, A., 2010. Anti-inflammatory and antipyretic effects of Sonchus oleraceus in rats. J. Ethnopharmacol. 127, 737–741.
Vinegar, R., Schreiber, W., Hugo, R., 1969. Biphasic development of carrageenan edema in rats. J. Pharmacol. Exp. Ther. 166, 96–103.
Yamamoto, T., Nozaki-Taguchi, N., Chiba, T., 2002. Analgesic effect of intrathecally administered orexin-A in the rat formalin test and in the rat hot plate test. Br. J. Pharmacol. 137, 170–176.
Zorn, B., García-Pi˜neres, A.J., Castro, V., Murillo, R., Mora, G., Merfort, I., 2001. 3-Desoxyanthocyanidins from Arrabidaea chica. Phytochemistry 56, 831–835.
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CQR, FCV, FVS-C and GPC participated in study concept and design, acquisition of data, analysis and interpretation of data, drafting of the manuscript, critical revision of the manuscript for important intellectual content. WV, AG-P and MHS participated in study concept and design, acquisition of data, analysis and interpretation of data, drafting of the manuscript, critical revision of the manuscript for important intellectual content, administrative, technical, or material support, and study supervision.
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da Rocha, C.Q., Vilela, F.C., Santa-Cecília, F.V. et al. Oleanane-type triterpenoid: an anti-inflammatory compound of the roots Arrabidaea brachypoda. Rev. Bras. Farmacogn. 25, 228–232 (2015). https://doi.org/10.1016/j.bjp.2015.03.005
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DOI: https://doi.org/10.1016/j.bjp.2015.03.005