Acute genotoxicity analysis in vivo of the aqueous extract of Maytenus guyanensis Amazonian chichuá

Abstract

The species Maytenus guyanensis Klotzsch ex Reissek, Celastraceae, present a wide variety of possible pharmacological activities and its roots and stems are used by popular medicine in the western Amazon rainforest. Few studies have demonstrated the genotoxic safety of the popular use of this species, and owing to this, the present study aimed to perform an analysis of the acute genotoxicity in vivo of the aqueous extract of M. guyanensis. Male and female mice from Mus musculus species, of weights ranging from 20 to 40 g, organized in eight groups with different treatments were used. The aqueous extracts of the bark of M. guyanensis were administered orally by gavage with 0.1 ml of the test substance per 10 g of the animal, followed by performance of comet assay in peripheral blood, PCE/NCE correlation and occurrence of micronuclei in the bone marrow. It was found that the aqueous extract of M. guyanensis, with ten times higher concentration than those used in ethnopharmacology, did not present genotoxic effect and, moreover, it has antigenotoxic action in mice treated acutely. Further studies regarding bioaccumulation and chronic effects of this species are suggested, in order to improve the understanding of its mechanism of action, ensuring the efficacy and safety of its utilization and developing phytotherapics and drugs.

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Authors

Contributions

DUOM (doctoral student) participated in all stages of the study, including statistical analysis and writing of the article. RAL (doctoral student), GMP and JBF (graduate student), participated in the preparation of the aqueous extract and article writing. FCS (doctoral student) and RCP contributed in the genotoxic analysis (treatment of animals, comet assay, correlation pce/nce and micronucleus analysis); JSLTM and VAF contributed to supervision and writing the manuscript.

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Correspondence to Dionatas Ulises de Oliveira Meneguetti.

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Meneguetti, D.U.d.O., Lima, R.A., da Silva, F.C. et al. Acute genotoxicity analysis in vivo of the aqueous extract of Maytenus guyanensis Amazonian chichuá. Rev. Bras. Farmacogn. 25, 164–169 (2015). https://doi.org/10.1016/j.bjp.2015.03.003

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Keywords

  • Mutagenicity
  • Maytenus guyanensis
  • Western Amazon