Anatomical investigations of Piper amalago (jaborandi-manso) for the quality control

Abstract

Piper amalago L., Piperaceae, popularly known as jaborandi-manso, is a shrub that spans a height of 2–7 m. It can be found in the regions of Southern America downward up to the south of Brazil. Traditionally it is used to treat digestive problems, heart problems, and burns. This study aims to conduct an anatomical investigation and analysis of the leaves and stems of P. amalago through electron scanning and optical micro techniques. The analysis showed that P. amalago has a hypostomatic leaf, with a subepidermal layer on its surface. There are grandular trichomes that resemble sacs, conic non-glandular trichomes, dorsiventral mesophyll, and a plano-convex midrib having a single vascular bundle in the center. The petiole is short with irregularly shaped and adaxially grooved. The stem is circular in shape and contains two circles of vascular bundles and a sclerenchymatic sheath in the perimedular region. These anatomical features of the Piper amalago’s leaves and stems make it easy to pick it out among other species of the Piper genus. This is helpful when conducting quality control process.

References

  1. Amorin, M., Paula, J.P., Silva, R.Z., Farago, P.V., Budel, J.M., 2014. Pharmacobotanical study of the leaf and stem of Mikania lanuginosa for its quality control. Rev. Bras. Farmacogn. 24, 531–537.

    CAS  Google Scholar 

  2. Albiero, A.L.M., Paoll, A.A.S., Souza, L.A., Mourão, K.S.M., 2005. Morfoanatomia dos órgãos vegetativos de Piper crassinervium H.B. & K. (Piperaceae). Acta Bot. Bras. 19, 305–312.

    Google Scholar 

  3. Albiero, A.L.M., Souza, L.A., Mourão, K.S.M., Almeida, O.J.G., Lopes, W.A.L., 2005. Morfo-anatomia do caule e da folha de Piper gaudichaudianum Kuntze (Piperaceae). Acta Farm. Bom 24, 550–554.

    Google Scholar 

  4. Albiero, A.L.M., Paoli, A.A.S., Souza, L.A., Mourão, K.S.M., 2006. Morfoanatomia dos órgãos vegetativos de Piper hispidum Sw (Piperaceae). Rev. Bras. Farmacogn 16, 379–391.

    Google Scholar 

  5. Baldoqui, D.C., Bolzani, V.S., Furlan, M., Kato, M.J., Marques, M.O.M., 2009. Flavonas lignanas e terpeno de Piper umbellata (Piperaceae). Quim. Nova. 32, 1107–1109.

    CAS  Google Scholar 

  6. Berlyn, G.P., Miksche, J., 1976. Botanical Microtechnique and Cytochemistry. lowa State University, Eames.

    Google Scholar 

  7. Budel, J.M., Duarte, M.R., 2008. Estudo farmacobotânico de folha e caule de Baccharis uncinella DC. Lat. Am. J. Pharm 27, 740–746.

    Google Scholar 

  8. Budel, J.M., Duarte, M.R., 2010. Macro and microscopic characters of the aerial vegetative organs of carqueja: Baccharis usterii Heering. Braz. Arch. Biol. Technol. 53, 123–131.

    Google Scholar 

  9. Camilotti, J.G., Biu, C.C., Farago, P., Santos, V.L.P., Franco, C.R.C., Budel, J.M., 2014. Anatomical characters of leave and stem of Calea serrata Less Asteraceae. Braz. Arch. Biol. Techn. 57, 867–873.

    Google Scholar 

  10. Carrara, V.S., Serra, L.Z., Cardozo-Filho, L., Cunha-Júnior, E.F., Torres-Santos, E.C., Cortez, D.A.G., 2012. HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity. Molecules 17, 15–33.

    CAS  Google Scholar 

  11. Carrara, V.S., Cunha-Júnior, E.F., Torres-Santos, E.C., Corrêa, A.G., Monteiro, J.L., Demarchi, I.G., Lonardoni, M.V.C., Cortez, D.A.G., 2013. Antileishmanial activity of amides from Piper amalago and synthetic analogs. Rev. Bras. Farmacogn. 23, 447–454.

    CAS  Google Scholar 

  12. Castro, M.M., Leitão-Filho, H.F., Monteiro, W.R., 1997. Utilização de estruturas secretoras na identificação dos gêneros de Asteraceae de uma vegetação de cerrado. Rev. Bras. Bot. 20, 163–174.

    Google Scholar 

  13. Chander, M.P., Kartick, C., Vijayachari, P., 2014. Ethnomedicinal knowledge among Karens of Andaman & Nicobar Islands, India. J. Ethnopharmacol. 162, 127–133.

    PubMed  Google Scholar 

  14. Cronquist, A., 1981. An Integrated System of Classification of Flowering Plants. Columbia University Press, New York.

    Google Scholar 

  15. Duarte, M.R., Siebenrock, M.C.N., 2010. Caracteres Anatômicos de Folha e Caule de Piper mikanianum (Kunth) Steud., Piperaceae. Lat. Am. J. Pharm. 29, 45–51.

    CAS  Google Scholar 

  16. Duke, S.O., 1994. Glandular trichomes: a focal point of chemical and structural interactions. Int. J. Plant Sci. 155, 617–620.

    Google Scholar 

  17. Facundo, V.A., Pollli, A.R., Rodrigues, R.V., Militão, J.S.L.T., Stabelli, R.G., Cardoso, C.T., 2008. Constituintes químicos fixos e voláteis dos talos e frutos de Piper tuberculatum Jacq e das raízes de P. hispidum H. B. K. Acta Amaz 38, 743–748.

    CAS  Google Scholar 

  18. Fahn, A., 1988. Secretory tissues in vascular plants. New Phytologis. 108, 229–257.

    Google Scholar 

  19. Figueiredo, R.A., Sazima, M., 2000. Pollination biology of Piperaceae species in southeastern Brazil. Ann. Bot. 85, 455–460.

    Google Scholar 

  20. Folquitto, D.G., Budel, J.M., Pereira, C.B., Brojan, L.E.F., Folquitto, G.G., Miguel, M.D., Silva, R., Miguel, Z.O.G., 2014. Analytical micrography and preliminary phytochemistry of the leaves and Stems of Lobelia exaltata Pohl (Campanulaceae). Lat. Am. J. Pharm. 33, 245–250.

    Google Scholar 

  21. Foster, A.S., 1949. Practical Plant Anatomy, 2nd ed. D. Van Nostrand, Princeton.

    Google Scholar 

  22. Franceschi, V.R., Horner-Junior, H.T., 1980. Calcium oxalate crystals in plants. Bot. Rev. 46, 361–427.

    CAS  Google Scholar 

  23. Gogosz, A.M., Boeger, M.R.T., Negrelle, R.R.B., Bergom, C., 2012. Anatomia foliar comparativa de nove espécies do gênero Piper (Piperaceae). Rodriguésia 63, 405–417.

    Google Scholar 

  24. Guimarães, E.F., Valente, C., 2001. Piperaceae–Piper. In: Reitz, R. (Ed.), Flora ilustrada catarinense. Herbário Barbosa Rodrigues, Itajaí.

    Google Scholar 

  25. Jasinski, V.C.G., Silva, R.Z., Pontarolo, R., Budel, J.M., Campos, F.R., 2014. Morpho-anatomical characteristics of Baccharis glaziovii in support of its pharmacobotany. Rev. Bras. Farmacogn. 24, 506–515.

    Google Scholar 

  26. Jaramillo, M.A., Manos, P.S., 2001. Filogenia e padrões de diversidade floral no gênero Piper (Piperaceae). Am. J. Bot 88, 706–716.

    CAS  Google Scholar 

  27. Johansen, D.A., 1940. Plant Microtechnique. MacGraw Hill Book, New York (NY).

    Google Scholar 

  28. Joly, A.B., 2002. Botânica: introdução à taxonomia vegetal. Nacional, São Paulo (SP).

    Google Scholar 

  29. Kato, E.T.M., Bacchi, E.M., Hernandes, L.S., 2012. Farmacobotânica e atividade antiúlcera de plantas medicinais brasileiras. In: Lopes, N.P., Souza, G.H.B., Mello, J.C.P. (Eds.), Farmacognosia: coletânea científica. UFOP, Ouro Preto, pp. 177–196.

    Google Scholar 

  30. Machado, N.S.O., (Tese de Doutorado) 2007. Estudo da anatomia foliar de espécies do gênero Piper L. (Piperaceae) no estado do Rio de Janeiro. Rio de Janeiro. Universidade Federal do Rio de Janeiro, 103 pp.

    Google Scholar 

  31. Metcalfe, C.R., Chalk, L., 1950. Anatomy of Dicotyledons: Leaves, Stem, and Woods in Relation to Taxonomy With Notes on Economic Uses, vol. 1. Clarendon, Oxford.

    Google Scholar 

  32. Metcalfe, C.R., Chalk, L., 1979. Anatomy of Dicotyledons, 2nd ed. Clarendon, Oxford.

    Google Scholar 

  33. Nakata, P.A., 2003. Advances in our understanding of calcium oxalate crystal formation and function in plants. Plant Sci. 164, 901–909.

    CAS  Google Scholar 

  34. Nascimento, M.E., Vilhena-Potiguara, C.R., 1999. Aspectos anatômicos dos órgãos vegetativos d de Piper hispidinervium C. DC. (Piperaceae) e suas estruturas secretoras. Bol. Mus. Para. Emílio Goeldi ser. Bot. 15, 39–104.

    Google Scholar 

  35. O’Brien, T.P., Feder, N., McCully, M.E., 1964. Polychromatic staining of plant cell walls by toluidine blue O. Protop 59, 368–373.

    Google Scholar 

  36. Oliveira, F., Akisue, G., Akisue, M.K., 2005. Farmacognosia. Atheneu, São Paulo.

    Google Scholar 

  37. Oliveira, A.M.A., Santos, V.L.P., Franco, C.R.C., Farago, P.V., Duarte, M.R., Budel, J.M., 2011. Comparative morpho-anatomical study of Baccharis curitybensis Heering ex Malme and Baccharis spicata (Lam.) Baill. Lat. Am. J. Pharm. 30, 1560–1566.

    Google Scholar 

  38. Parmar, V.S., Jain, S.C., Bisht, S.K., Jain, R., Taneja, P., Tyagi, O.D., 1997. Phytochemistry of the genus Piper. Phytochemistry 46, 591–673.

    Google Scholar 

  39. Pereira, C.B., Farago, P., Budel, J.M., Padilha de Paula, J., Folquitto, D.G., Miguel, O.G., Miguel, M.D., 2014. Contribution to the pharmacognostic study of Carquejas: Baccharis milleflora DC., Asteraceae. Lat. Am. J. Pharm. 33, 841–847.

    Google Scholar 

  40. Pereira, C.B., Miguel, M.D., Folquitto, D.G., Miguel, O.G., Farago, P.V., Paula, J.P., Santos, V.L.P., Franco, C.R.C., Budel, J.M., 2014. Architecture of the aerial vegetative organs and scanning electron micrographs of Dioscorea bulbifera L for the quality control. Lat. Am. J. Pharm. 33, 1100–1105.

    Google Scholar 

  41. Pessini, G.L., Holetz, F.B., Sanches, N.R., Cortez, D.A.G., Filho, B.D., Nakamura, C.V., 2003. Avaliação da atividade antibacteriana e antifúngica de extratos de plantas utilizados na medicina popular. Rev. Bras. Farmacogn. 13, 21–24.

    Google Scholar 

  42. Raman, V., Galal, A.M., Khan, I.A., 2012. An Investigation of the vegetative anatomy of piper sarmentosum and a comparison with the anatomy of Piper betle (Piperaceae). Am. J. Plant Sei. 3, 1135–1144.

    Google Scholar 

  43. Ravindran, P.N., Remarshree, A.B., 1998. Anatomy of Piper colubrinum link. J. Spices Aromat. Crops 7, 11–123.

    Google Scholar 

  44. Regasini, L.O., Cotinguiba, F., Passerini, G.D., Bolzani, V.S., Cicarelli, R.M., Kato, M.J., Furlan, M., 2009. Trypanocidal activity of Piper arboreum and Piper tuberculatum (Piperaceae). Rev. Bras. Farmacogn. 19, 199–203.

    CAS  Google Scholar 

  45. Regasini, L.O., Cotinguiba, F., Morandim, A.A., Kato, M.J., Scorzoni, L., Mendes-Giannini, M.J., Bolzani, V.S., Furlan, M., 2009. Antimicrobial activity of Piper arboreum and Piper tuberculatum (Piperaceae) against opportunistic yeasts. Afr. J. Biotechnol. 17, 2866–2870.

    Google Scholar 

  46. Rocha, W.S., Lopes, R.M., Silva, D.B., Vieira, R.F., Silva, J.P., Agostini-Costa, T.S., 2011. Compostos fenólicos totais e taninos condensados em frutas nativas do cerrado. Rev. Bras. Frutic. 33, 1215–1221.

    Google Scholar 

  47. Roeser, K.R., 1972. Die Nadel der Schwarzkiefer-Massenprodukt und Kunstwerk der Natur. Mikrokosmos 6, 33–36.

    Google Scholar 

  48. Rovani, G., Santos, V.L.P., Miguel, O.G., Budel, J.M., Campos, R., 2013. Investigação fitoquímica e antioxidante de partes vegetativas aéreas de Piper amalago L. Cad. Esc. Saúde Unibrasil 2, 164–177.

    Google Scholar 

  49. Santiago, E.J.A., Pinto, J.E.B.P., Castro, E.M., Lameira, A.O., Conceição, H.E.O., Gavilanes, M.L., 2001. Aspectos da anatomia foliar da pimenta-longa (Piper hispidinervium C DC.) sob diferentes condições de luminosidade. Ciênc. Agrotec. 25, 1035–1042.

    Google Scholar 

  50. Sass, J.E., 1951. Botanical Microtechnique, 2nd ed. Iowa State College, Ames.

    Google Scholar 

  51. Silva, E.M.J., Machado, S.R., 1999. Estrutura e desenvolvimento dos tricomassecretores em folhas de Piper regnellii (Miq) C. DC. var. regnellii (Piperaceae). Rev. Bras. Bot. 22, 117–124.

    Google Scholar 

  52. Silva, D.M.H., Bastos, C.N., 2007. Atividade antifúngica de óleos essenciais de espécies de Piper sobre Crinipellis perniciosa Phytophthora palmivora e Phytophthora capsici. Fitopatol. Bras. 32, 143–145.

    Google Scholar 

  53. Silva, W.C., Ribeiro, J.D.A., Menezes de Souza, H.E., Correa, R.D.S., 2007. Insecticidal activity of Piper aduncum L. (Piperaceae) on Aetalion sp (Hemiptera: Aetalionidae), plague of economic importance in Amazon. Acta Amazonica 37, 293–298.

    Google Scholar 

  54. Smith, A.M., 2010. Starch and starch granules. Gen. Introductory Life Sci., http://dx.doi.org/10.1002/9780470015902.a0001294.pub2.

    Google Scholar 

  55. Souza, W., 1998. Técnicas básicas de microscopia eletrônica aplicada as Ciências Biológicas. Sociedade Brasileira de Microscopia Eletrônica, Rio de Janeiro (RJ).

    Google Scholar 

  56. Souza, L.A., Moscheta, I.S., Oliveira, J.H.G., 2004. Comparative morphology and anatomy of the leaf and stem of Peperomia dahlstedtii, Ottonia martiana and Piper diospyrifolium (Piperaceae). Gayana Bot. 6, 6–17.

    Google Scholar 

  57. Souza, V.C., 2005. Botânica sistemática. Plantarum, Nova Odessa (SP).

    Google Scholar 

  58. Souza, V.C., Lorenzi, H., 2005. Botânica Sistemática: guia ilustrado para identificação das famílias de angiospermas da flora brasileira, baseado em APG II. Nova Oddessa, São Paulo. Instituto Plantarum, 230–231.

    Google Scholar 

  59. Souza, L.A., Albiero, A.L.M., Almeida, O.J.G., Lopes, W.A.L., Mourão, K.S.M., Moscheta, I.S., 2009. Estudo morfo-anatômico da folha e do caule de Piper arboreum Aubl (Piperaceae). Lat. Am. J. Pharm. 28, 103–107.

    Google Scholar 

  60. Squena, A.P., Santos, V.L.P., Franco, C.R.C., Budel, J.M., 2012. Análise morfoanatômica de partes vegetativas aéreas de Pereskia aculeata Mill., Cactaceae. Cad. Esc. Saúde Unibrasil 8, 189–207.

    Google Scholar 

  61. Takemori, N.K., Bona, C., Alquini, Y., 2003. Anatomia comparada das folhas de espécies de Peperomia (Piperaceae)–I Ontogênese do tecido aquífero e dos estômatos. Acta Bot. Bras. 17, 387–394.

    Google Scholar 

  62. Tissier, A., 2012. Glandular trichomes: what comes after expressed sequence tags? Plant J. 70, 51–68.

    CAS  PubMed  Google Scholar 

  63. Vianna, W.O., Akisue, G., 1997. Caracterização morfológica de Piper aduncum L. Lecta 15, 11–62.

    Google Scholar 

  64. Wagner, G.J., 1991. Secreting glandular trichomes: more than just hairs. Plant Physiol. 96, 675–679.

    CAS  PubMed  PubMed Central  Google Scholar 

  65. Youssef, J., Döll-Boscardin, P.M., Farago, P.V., Duarte, M.R., Budel, J.M., 2013. Gochnatia polymorpha: macro-and microscopic identification of leaf and stem for pharmacognostic quality control. Rev. Bras. Farmacogn. 23, 585–591.

    Google Scholar 

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Contributions

VLPS assisted in collecting and identifying plant samples, making a herbarium, conducting laboratory experiments, data analysis and drafting the paper. JMB supervised the laboratory work. IJMR undertook a critical reading of the manuscript. CRCF assisted in the scanning electron microscopy (SEM) analysis. EA was responsible for preparing the permanent slides. All the authors have read the final manuscript and agreed to its submission for appraisal.

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Correspondence to Vera L. P. dos Santos.

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dos Santos, V.L.P., Franco, C.R.C., Amano, E. et al. Anatomical investigations of Piper amalago (jaborandi-manso) for the quality control. Rev. Bras. Farmacogn. 25, 85–91 (2015). https://doi.org/10.1016/j.bjp.2015.03.001

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Keywords

  • Anatomy
  • Quality control
  • Jaborandi-manso
  • Piper amalago
  • Piperaceae