Autotoxicity in Pogostemon cablin and their allelochemicals

Abstract

The effects of allelochemicals and aqueous extracts from different Pogostemon cablin (Blanco) Benth., Lamiaceae, parts and rhizosphere soil on growth parameters, leaf membrane peroxidation and leaf antioxidant enzymes were investigated in patchouli. P. cablin seedlings were incubated in solutions containing allelochemicals and aqueous extracts from different patchouli parts and its rhizosphere soil at several concentrations. Firstly, the growth parameters were significantly reduced by the highest concentration of leaves, roots and stems extracts (p < 0.05). As compared to the control, plant height was reduced by 99.8% in the treatment with leaves extracts (1:10). The malondialdehyde content increased greatly when patchouli seedlings were subject to different concentrations of leaves, roots and stems extracts; meanwhile, the superoxide dismutase and peroxidase activities showed an increase trend at the low concentration, followed by a decline phase at the high concentration of roots and leaves extracts (1:10). What’s more, leaves and roots extracts had a more negative effect on patchouli growth than stems extracts at the same concentrations. Secondly, the total fresh mass, root length and plant height were greatly reduced by the highest strength of soil extracts. Their decrements were 22.7, 74.9, and 33.1%, respectively. Thirdly, growth parameters and enzymatic activities varied considerably with the kinds of allelochemicals and with the different concentrations. Plant height, root length and total fresh weight of patchouli were greatly reduced by p-hydroxybenzoic acid (200 μM), and their decrements were 77.0, 42.0 and 70.0%, respectively. Finally, three useful measures on reducing the autotoxicity during the sustainable patchouli production were proposed.

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Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (81360618 and 31360210), The Specialized Fund for the Modernization of Traditional Chinese Medicine of Hainan Province (ZY201413), The State Key Subject of Botany at Hainan University (071001), Academic Discipline Construction Project Plan in the Central and Western Regions of Hainan University (ZXBJH-XK008).

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All authors contributed in collecting the plant sample, running the laboratory work, analyzing the data and drafting the manuscript. All authors participated in revising the article critically.

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Correspondence to You-Gen Wu.

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Xu, Y., Wu, Y., Chen, Y. et al. Autotoxicity in Pogostemon cablin and their allelochemicals. Rev. Bras. Farmacogn. 25, 117–123 (2015). https://doi.org/10.1016/j.bjp.2015.02.003

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Keywords

  • Autotoxicity
  • Allelochemicals
  • Enzymatic activity
  • Pogostemon cablin
  • Rhizosphere soil