Advertisement

Mammalian Biology

, Volume 75, Issue 1, pp 69–73 | Cite as

Detection of hybrids between wild boars (Sus scrofa scrofa) and domestic pigs (Sus scrofa f. domestica) in Greece, using the PCR-RFLP method on melanocortin-1 receptor (MC1R) mutations

  • Evagelia A. Koutsogiannouli
  • Katerina A. Moutou
  • Theologia Sarafidou
  • Costas Stamatis
  • Zissis MamurisEmail author
Original Investigation

Abstract

The melanocortin-1 receptor (MC1R) regulates melanogenesis in mammals within the mammalian melanocyte and the hair follicle. Common variations (polymorphisms) in the MC1R gene are associated with normal differences in skin and hair colour. So far, a unique MC1R allele (E+) has been identified in European wild boar (Sus scrofa scrofa), associated with the wild-type coat colour (variable shades of brown) that is not found in any of the domestic breeds. In addition, a series of alleles found in pigs, some of which observed only in particular breeds, have been proposed as markers in breed traceability systems. The current study is an attempt to detect possible hybrids between wild boars and domestic pig breeds as well as to identify races of pig that are not purebred. For this purpose, wild boars were analysed against Large White pigs, applying the PCR-restriction fragment length polymorphism (RFLP) method. A high percentage (16.7%) of hybrids was detected within a breeding station compared with the percentage of hybrids within the populations of free-ranging wild boar (5.0%). These results should be taken into consideration for future restocking operations to avoid the chance of outbreeding depression, which is more intense when local populations are introgressed by gene pools from domesticated, usually inbred, animals.

Keywords

MC1R hybrids Sus scrofa 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson, L., 2003. Melanocortin receptor variants with phenotypic effects in horse, pig and chicken. Ann. N.Y. Acad. Sci. 994, 313–318.CrossRefGoogle Scholar
  2. Booth, W., 1988. Reintroducing a political animal. Science 241, 156–158.CrossRefGoogle Scholar
  3. Brodmann, P.D., Nicholas, G., Schaltenbrand, P., Ilg, E.C., 2001. Identifying unknown game species: experience with nucleotide sequencing of the mitochondrial cytochrome b gene and a subsequent basic local alignment search tool search. Eur. Food Res. Technol. 212, 491–496.CrossRefGoogle Scholar
  4. Conant, S., 1988. Saving endangered species by translocation. Are we tinkering with evolution? BioScience 38, 254–257.CrossRefGoogle Scholar
  5. D’Alessandro, E., Fontanesi, L., Liotta, L., Davoli, R., Chiofalo, V., Russo, V., 2007. Analysis of the MC1R gene in the Nero Siciliano pig breed and usefulness of this locus for breed traceability. Vet. Res. Commun. 31, 389–392.CrossRefGoogle Scholar
  6. Fajardo, V., González, I., Martín, I., Rojas, M., Hernández, P.E., García, T., Martín, R., 2008. Differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR analysis targeting the mitochondrial D-loop and the nuclear melanocortin receptor 1 (MC1R) genes. Meat Sci. 78, 314–322.CrossRefGoogle Scholar
  7. Fernandez-de-Mera, I.G., Gortazar, C., Vicente, J., Höfle, U., Fierro, Y., 2003. Wild boar helminths: risks in animal translocations. Vet. Parasitol. 115, 335–341.CrossRefGoogle Scholar
  8. Fontanesi, L., Tazzoli, M., Beretti, F., Russo, V., 2006. Mutations in the melanocortin 1 receptor (MC1R) gene are associated with coat colours in the domestic rabbit (Oryctolagus cuniculus). Anim. Genet. 37, 489–193.CrossRefGoogle Scholar
  9. Frankham, R., 1995. Conservation genetics. Annu. Rev. Genet. 29, 305–327.CrossRefGoogle Scholar
  10. Griffith, B., Scott, J.M., Carpenter, J.W., Reed, C., 1989. Translocations as a species conservation tool: status and strategy. Science 245, 477–480.CrossRefGoogle Scholar
  11. Haig, S.M., 1998. Molecular contribution to conservation. Ecology 79, 413–425.CrossRefGoogle Scholar
  12. Herrmann, B.G., Frischauf, A.M., 1987. Isolation of genomic DNA, method. Enzymologia 152, 180–183.CrossRefGoogle Scholar
  13. Jeon, J.T., Lee, J.H., Kim, K.S., Park, C.K., Oh, S.J., 2006. Application of DNA markers in animal industries. Aust. J. Exp. Agric. 46, 173–182.CrossRefGoogle Scholar
  14. Kijas, J.H.M., Wales, R., Törnsten, A., Chardon, P., Mollet, M., Andersson, L., 1998. Melanocortin receptor 1 (MC1R) mutations and coat color in pigs. Genetics 150, 1177–1185.PubMedPubMedCentralGoogle Scholar
  15. Kleiman, D.G., 1989. Reintroduction of captive mammals for conservation. BioScience 39, 152–161.CrossRefGoogle Scholar
  16. Klungland, H., Våge, D.I., Gomez-Raya, L., Adalsteinsson, S., Lien, E., 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat colour determination. Mamm. Genome 6, 636–639.CrossRefGoogle Scholar
  17. Laws, G.M., Adams, S.P., 1996. Measurement of 8-OHdG in DNA by HPLC/ECD: the importance of DNA purity. BioTechniques 20, 36–38.CrossRefGoogle Scholar
  18. Lynch, M., O’Hely, M., 2001. Captive breeding and the genetic fitness of natural populations. Conserv. Genet. 2, 363–378.CrossRefGoogle Scholar
  19. Marklund, L., Johansson Moller, M., Sandberg, K., Andersson, L., 1996. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat colour in horses. Mamm. Genome 7, 895–899.CrossRefGoogle Scholar
  20. Naya, Y., Horiuchi, M., Ishiguro, N., Shinagawa, M., 2003. Bacteriological and genetic assessment of game meat from Japanese wild boars. J. Agric. Food Chem. 51, 345–349.CrossRefGoogle Scholar
  21. Robbins, L.S., Nadeau, J.H., Johnson, K.R., Kelly, M.A., Roselli-Rehfuss, L., Baack, E., Mountjoy, K.G., Cone, R.D., 1993. Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function. Cell 72, 827–834.CrossRefGoogle Scholar
  22. Russo, V., Fontanesi, L., Scotti, E., Tazzoli, M., Dall’Olio, S., Davoli, R., 2007. Analysis of melanocortin 1 receptor (MC1R) gene polymorphisms in some cattle breeds: their usefulness and application for breed traceability and authentication of Parmigiano Reggiano cheese. Ital. J. Anim. Sci. 6, 257–272.CrossRefGoogle Scholar
  23. Scandura, M., Iacolina, L., Crestanello, B., Pecchioli, E., Di Benedetto, M.F., Russo, V., Davoli, R., Apollonio, M., Bertorelle, G., 2008. Ancient vs. recent processes as factors shaping the genetic variation of the European wild boar: are the effects of the last glaciation still detectable? Mol. Ecol. 17, 1745–1762.CrossRefGoogle Scholar
  24. Scott, J.M., Carpenter, J.W., 1987. Release of captive-reared or translocated endangered birds: what do we need to know? Auk 104, 544–545.CrossRefGoogle Scholar
  25. Seddon, P.J., 1999. Persistence without intervention: assessing success in wildlife reintroduction. Trends Ecol. Evol. 14, 503.CrossRefGoogle Scholar
  26. Storfer, A., 1998. Gene flow and endangered species translocations: a topic revisited. Biol. Conserv. 87, 173–180.CrossRefGoogle Scholar
  27. Teletchea, F., Maudet, C., Hanni, C., 2005. Food and forensic molecular identification: update and challenges. Trends Biotechnol. 23, 359–366.CrossRefGoogle Scholar
  28. Vage, D.I., Klungland, H., Lu, D., Cone, R.D., 1999. Molecular and pharmacological characterization of dominant black coat color in sheep. Mamm. Genome 10, 39–43.CrossRefGoogle Scholar
  29. Valverde, P., Healy, E., Jackson, I., Rees, J.L., Thody, A.J., 1995. Variants of the melanocyte-stimulating hormone receptor gene are associated with red hair and fair skin in humans. Nat. Genet. 11, 328–330.CrossRefGoogle Scholar

Copyright information

© Deutsche Gesellschaft für Säugetierkunde 2008

Authors and Affiliations

  • Evagelia A. Koutsogiannouli
    • 1
  • Katerina A. Moutou
    • 1
  • Theologia Sarafidou
    • 1
  • Costas Stamatis
    • 1
  • Zissis Mamuris
    • 1
    Email author
  1. 1.Department of Biochemistry and BiotechnologyUniversity of ThessalyLarissaGreece

Personalised recommendations