Journal of Bionic Engineering

, Volume 5, Issue 4, pp 308–316 | Cite as

Microfabrication of Bubbular Cavities in PDMS for Cell Sorting and Microcell Culture Applications

  • Ut-Binh T. Giang
  • Michael R. King
  • Lisa A. DeLouiseEmail author


We describe a novel technique, low surface energy Gas Expansion Molding (GEM), to fabricate microbubble arrays in polydimethylsiloxane (PDMS) which are incorporated into parallel plate flow chambers and tested in cell sorting and microcell culture applications. This architecture confers several operational advantages that distinguish this technology approach from currently used methods. Herein we describe the GEM process and the parameters that are used to control microbubble formation and a Vacuum-Assisted Coating (VAC) process developed to selectively and spatially alter the PDMS surface chemistry in the wells and on the microchannel surface. We describe results from microflow image visualization studies conducted to investigate fluid streams above and within microbubble wells and conclude with a discussion of cell culture studies in PDMS.


polydimethylsiloxane microfabrication cell culture cell sorting molding 



the spheroidal geometry exhibited by the formation of microbubbles.


polydimethylsiloxane polymer


bubbular structures molded into PDMS with an opening(s) on the side planar to the PDMS surface.


gas expansion molding, a new technique developed to form microbubbles in PDMS.


vacuum-assisted coating, a new technique developed to coat the interior wall of microbubbles in PDMS.


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  1. [1]
    Korin N, Bransky A, Dinnar U, Levenberg S. A parametric study of human fibroblasts culture in a microchannel bioreactor. Lab on a Chip, 2007, 7, 611–617.CrossRefGoogle Scholar
  2. [2]
    Hung P J, Le P J, Sabounchi P, Aghdam N, Lin R, Lee L P. A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array. Lab on a Chip, 2005, 5, 44–48.CrossRefGoogle Scholar
  3. [3]
    Zhang Z, Boccazzi P, Choi H G, Perozziello G, Sinskey A J, Jensen K F. Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor. Lab on a Chip, 2006, 6, 906–913.CrossRefGoogle Scholar
  4. [4]
    Kim L, Toh Y C, Voldman J, Yu H. A practical guide to microfluidic perfusion culture of adherent mammalian cells. Lab on a Chip, 2007, 7, 681–694.CrossRefGoogle Scholar
  5. [5]
    Gu W, Zhu X, Futai N, Cho B S, Takayama S. Computerized microfluidic cell culture using elastomeric channels and Braille displays. PNAS, 2004, 101, 15861–15866.CrossRefGoogle Scholar
  6. [6]
    Takagi J, Yamada M, Yasuda M, Seki M. Continuous particle separation in a microchannel having asymmetrically arranged multiple branches. Lab on a Chip, 2005, 5, 778–784.CrossRefGoogle Scholar
  7. [7]
    Yamada M, Seki M. Microfluidic particle sorter employing flow splitting and recombining. Analytical Chemistry, 2006, 78, 1357–1362.CrossRefGoogle Scholar
  8. [8]
    Charles N, Liesveld J L, King M R. Investigating the feasibility of stem cell enrichment mediated by immobilized selectins. Biotechnology Progess, 2007, 23, 1463–1472.CrossRefGoogle Scholar
  9. [9]
    Pappas D, Wang K. Cellular separations: A review of new challenges in analytical chemistry. Analytica Chimica Acta, 2007, 601, 26–35.CrossRefGoogle Scholar
  10. [10]
    Chin V I, Taupin P, Sanga S, Scheel J, Gage F H, Bhatia S N. Microfabricated platform for studying stem cell fates. Biotechnology and Bioengineering, 2004, 88, 399–415.CrossRefGoogle Scholar
  11. [11]
    Kwon K W, Choi S S, Lee S H, Kim B, Lee S N, Park M C, Kim P, Hwang S Y, Suh K Y. Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference. Lab on a Chip, 2007, 7, 1461–1468.CrossRefGoogle Scholar
  12. [12]
    Chang C, Wang Y F, Kanamori Y, Shih J J, Kawai Y, Lee C K, Wu K C, Esashi M. Etching submicrometer trenches by using the Bosch process and its application to the fabrication of antireflection structures. Journal of Micromechanics and Microengineering, 2005, 15, 580–585.CrossRefGoogle Scholar
  13. [13]
    Petillo O, Peluso G, Ambrosio L, Nicolais L, Kao W J, Anderson J M. In vivo induction of macrophage Ia antigen (MHC class 11) expression by biomedical polymers in the cage implant system. Journal of Biomedical Materials Research, 1994, 28, 635–646.CrossRefGoogle Scholar
  14. [14]
    Folch A, Ayon A, Hurtado O, Schmidt M A, Toner M. Molding of deep polydimethylsiloxane microstructures for microfluidics and biological applications. Journal of Biomechanical Engineering, 1999, 121, 28–34.CrossRefGoogle Scholar
  15. [15]
    Hediger S, Sayah A, Horisberger J, Gijs M A M. Modular microsystem for epithelial cell culture and electrical characterisation. Biosensors and Bioelectronics, 2001, 16, 689–694.CrossRefGoogle Scholar
  16. [16]
    Giang U T, Lee D, King M R, DeLouise L A. Microfabrication of cavities in polydimythylsiloxane using DRIE silicon molds. Lab on a Chip, 2007, 7, 1660–1662.CrossRefGoogle Scholar
  17. [17]
    Lee D, Rana K, Lee K, DeLouise L A, King M R. Microfabricated cavities for adhesive capture of stem cells under flow. Proceedings of the 5th International Conference on Nanochannels, Microchannels and Minichannels, Puebla, Mexico, 2007, 30177.Google Scholar
  18. [18]
    Wojciechowski J C, Narasipura S D, Charles N, Mickelsen D, Rana K, Blair M L, King M R. Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device. British Journal of Haematology, 2008, 140, 673–681.CrossRefGoogle Scholar

Copyright information

© Jilin University 2008

Authors and Affiliations

  • Ut-Binh T. Giang
    • 1
  • Michael R. King
    • 2
  • Lisa A. DeLouise
    • 1
    • 3
    Email author
  1. 1.Department of Biomedical EngineeringUniversity of RochesterRochesterUSA
  2. 2.Department of Biomedical EngineeringCornell UniversityIthacaUSA
  3. 3.Department of DermatologyUniversity of Rochester Medical CenterRochesterUSA

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