Microfabrication of Bubbular Cavities in PDMS for Cell Sorting and Microcell Culture Applications
We describe a novel technique, low surface energy Gas Expansion Molding (GEM), to fabricate microbubble arrays in polydimethylsiloxane (PDMS) which are incorporated into parallel plate flow chambers and tested in cell sorting and microcell culture applications. This architecture confers several operational advantages that distinguish this technology approach from currently used methods. Herein we describe the GEM process and the parameters that are used to control microbubble formation and a Vacuum-Assisted Coating (VAC) process developed to selectively and spatially alter the PDMS surface chemistry in the wells and on the microchannel surface. We describe results from microflow image visualization studies conducted to investigate fluid streams above and within microbubble wells and conclude with a discussion of cell culture studies in PDMS.
Keywordspolydimethylsiloxane microfabrication cell culture cell sorting molding
the spheroidal geometry exhibited by the formation of microbubbles.
bubbular structures molded into PDMS with an opening(s) on the side planar to the PDMS surface.
gas expansion molding, a new technique developed to form microbubbles in PDMS.
vacuum-assisted coating, a new technique developed to coat the interior wall of microbubbles in PDMS.
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