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Journal of Bionic Engineering

, Volume 5, Issue 3, pp 239–246 | Cite as

Using a Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Chicken Carcass

  • Yu-bin LanEmail author
  • Shi-zhou Wang
  • Yong-guang Yin
  • W. Clint Hoffmann
  • Xian-zhe Zheng
Article

Abstract

Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodborne pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically “taste” the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody concentrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 × 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.

Keywords

biosensor Salmonella Typhimurium food safety chicken carcass detection limitation Surface Plasmon Resonance 

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References

  1. [1]
    Green D K. Sterilization Apparatus, US Patent No. 4457221, 1987.Google Scholar
  2. [2]
    Food Safety: The Agricultural Use of Antibiotics and its Implications for Human Health, Letter Report, GAO/RCED-99-74, USDA, 1999.Google Scholar
  3. [3]
    Seo K H, Brackett R E, Hartman N F, Campbell D P. Development of a rapid response biosensor for detection of Salmonella Typhimurium. Journal of Food Protection, 1999, 62, 431–437.CrossRefGoogle Scholar
  4. [4]
    Fratamico P M, Strobaugh T P, Medina M B, Gehring A G. Detection of Escherichia Coli O157:H7 using a surface plasmon resonance biosensor. Biotechnology Techniques, 1998, 12, 571–576.CrossRefGoogle Scholar
  5. [5]
    Elkind J L, Stimpson D I, Strong A, Bartholomew D U, Melendez J L. Integrated analytical sensors: The use of the TISPR-1 as a biosensor. Sensor and Actuators B:Chemical, 1999, 54, 182–190.CrossRefGoogle Scholar
  6. [6]
    Buzby J C, Roberts T. Microbial foodborne illness. Choices, First Quarter, 1996, 14–17.Google Scholar
  7. [7]
    Buzby J, Roberts T, Liu C T, MacDonald J. Bacterial Foodborne Disease: Medical Costs and Productivity Losses, USDA Agricultural Rural Report, No. 741, 1996.Google Scholar
  8. [8]
    Lillard H S. The impact of commercial processing procedures on bacterial contamination and cross-contamination of broiler carcases. Journal of Food Protection, 1990, 53, 202–204.CrossRefGoogle Scholar
  9. [9]
    Center for Food Safety and Applied Nutrition, [2008-05-06], http://vm.cfsan.fda.gov
  10. [10]
    Meeusen C. Evaluation of a Surface Plasmon Resonance Biosensor for the Identification of Salmonella Typhimurium and Escherichia Coli O157:H7 in the Pork Production Chain, M.Sc. Thesis, Michigan State University, East Lasing, MI, 2000.Google Scholar
  11. [11]
    Moutville T J. Food Microbiology, CRC Press, NY, 1987.Google Scholar
  12. [12]
    Jay J M. Modern Food Microbiology, Sixth Edition, Aspen Publishers, Inc. Gaithersburg, MD, 2000.CrossRefGoogle Scholar
  13. [13]
    Deshpande S S. Immunodiagnostics in agricultural, food, and environmental quality control. Food Technology, 1994, 48, 136–141.Google Scholar
  14. [14]
    Turner A P, Newman J D. An Introduction to Biosensors, Biosensors for Food Analysis, Scott A O (ed), Athenaeum Press Ltd, Gateshead, UK, 1998.Google Scholar
  15. [15]
    Jonsson U. Optical Affinity Biosensors, Biosensors for Food Analysis, Scott A O (ed.) Athenaeum Press Ltd, Gateshead, UK, 1998.Google Scholar
  16. [16]
    Operation Manual, Spreeta Experimenter’s Kit, Texas Instruments, Inc. Dallas, Texas, USA, 1999.Google Scholar
  17. [17]
    Salamon Z, Brown M F, Tollin G. Plasmon resonance spectroscopy: Probing molecular interactions within membranes. Trends in Biochemical Sciences, 1999, 24, 213–219.CrossRefGoogle Scholar
  18. [18]
    Cranfield Biotechnology Center, [2008-04-21], http://www.cranfieldac.uk/biotech/spr.htm
  19. [19]
    Demarco D, Saaski E, McCrae D, Lim D. Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber optic biosensor. Journal of Food Protection, 1999, 62, 711–716.CrossRefGoogle Scholar
  20. [20]
    Meeusen C, Alocilja E, Osburn W. Detection of pathogenic bacteria in the pork industry chain using a surface plasmon resonance biosensor. Part 1: Sensor verification. 93rd Annual International Meeting of ASAE, Milwaukee, Wisconsin, USA, 2000, paper no. 006046.Google Scholar
  21. [21]
    Meeusen C, Alocilja E, Osburn W. Evaluation of a surface plasmon resonance biosensor in the detection of Salmonella spp. ASAE, St. Joseph, MI, 2001a, paper no. 016035.Google Scholar
  22. [22]
    Meeusen C, Alocilja E Osburn, Ryser E. Use of biosensor for pathogen monitoring in the pork production chain. ASAE, St. Joseph, MI, 2001b, paper no. 017031.Google Scholar
  23. [23]
    Meeusen C, Alocilja E, Osburn W. Detection of E. coli O157:H7 using a surface plasmon resonance biosensor. ASAE, St. Joseph, MI, 2001c, paper no. 017030.Google Scholar
  24. [24]
    Wortberg M, Orban M, Renneberg R, Cammann K. Fluorimetric immunosensors. In Kress-Rogers (ed.), Handbook of Biosensors and Electronic Noses, CRC, Boca Raton, Florida, 1997.Google Scholar

Copyright information

© Jilin University 2008

Authors and Affiliations

  • Yu-bin Lan
    • 1
    Email author
  • Shi-zhou Wang
    • 2
  • Yong-guang Yin
    • 3
  • W. Clint Hoffmann
    • 1
  • Xian-zhe Zheng
    • 4
  1. 1.USDA-ARSCollege StationUSA
  2. 2.Department of Environmental and Occupational HealthTexas A&M UniversityCollege StationUSA
  3. 3.College of Biological and Agricultural EngineeringJilin UniversityChangchunP. R. China
  4. 4.College of EngineeringNortheast Agricultural UniversityHarbinP. R. China

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