Development of a LC-MS/MS Method for the Quantification of Myricitrin: Application to a Pharmacokinetic Study in Rats

Revista Brasileira de Farmacognosia volume 31, pages102–106(2021)Cite this article

Abstract

A highly sensitive reversed-phase liquid chromatography coupled to tandem mass spectrometry for the quantification of myricitrin in rat plasma was developed and validated using orientin as an internal standard. The detection was achieved on a triple-quadrupole mass spectrometer using selected reaction monitoring mode in the negative electrospray ionization source. Linearity was constructed over a concentration range of 2.0–1000 ng/ml for myricitrin. The sensitivity and accuracy of the method allowed determination of the pharmacokinetics of myricitrin in rats following intravenous administration in male rats.

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Affiliations

  1. Jiaozhou People’s Hospital, Qingdao, 266300, China

    Yan Wang, Youjin Fan & Hong Wang

  2. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China

    Zhipeng Deng

  3. Shandong Medical College, Jinan, 250002, China

    Weihong Wu

  4. Institute of Material Medical, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250062, China

    Jia Liu

Authors
  1. Yan Wang
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  2. Youjin Fan
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  3. Zhipeng Deng
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  4. Weihong Wu
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  5. Jia Liu
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  6. Hong Wang
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Correspondence to Hong Wang.

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Wang, Y., Fan, Y., Deng, Z. et al. Development of a LC-MS/MS Method for the Quantification of Myricitrin: Application to a Pharmacokinetic Study in Rats. Rev. Bras. Farmacogn. 31, 102–106 (2021). https://doi.org/10.1007/s43450-020-00121-6

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Keywords

  • Flavonoid glycoside
  • Liquid chromatography
  • Method validation
  • Myricitrin
  • Myrycaceae
  • Tandem mass spectrometry