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Wound healing activity and chemical composition of geopropolis from Melipona subnitida

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Abstract

Geopropolis collected by stingless bees is a product widely used in the semiarid Northeast region of Brazil. The wound healing activity, palynological analysis, free-radical scavenging activities, and the chemical composition of the hydroalcoholic extract of six samples from jandaíra geopropolis were determined. The wound healing activity was evaluated using 10% geopropolis hydroalcoholic extract added to the formulation incorporated into a base of Lannette cream, and the chemical composition of six samples was determinate by ultra-performance liquid chromatography coupled with diode array and quadrupole time-of-flight mass spectrometry. The detected compounds in the five samples (1–5) were classified as flavonoid aglycones (flavonols, flavanonols, flavonones, flavones, and chalcones). In sample 6, flavonoids, phenylpropanoids, and hydrolysable tannins were identified. Analysis of the pollen types in geopropolis showed that pollen from Leguminosae was predominant, indicating the visit of bees in the species of this family. This sample (6), with the highest content of phenolic compounds and high free-scavenging activity, was tested to evaluate the wound healing potential. The topical application of geopropolis (10%) increased the wound healing rate and re-epithelialization of wounds in rats. Our data indicate that the mixture of phenolic components in the geopropolis may contribute to the activities of free-radical scavenging and wound healing.

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Funding

This work was financially supported by grants from CNPq (Grant no. 454249/2014-4 and 301935/2018-1), FACEPE (Grant no. PRONEM APQ-0741106/2014) and CAPES. Special thanks to the presidents of Brazil Luiz Inácio Lula da Silva and Dilma Van Rousseff who encouraged the research in Brazil through financing of projects.

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Authors and Affiliations

  1. Departmento de Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil

    Déborah M. N. de Sousa-Fontoura, Roberio G. Olinda, Geysa A. Viana & Jael S. Batista

  2. Laboratório de Bioprospecção Fitoquímica, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil

    Déborah M. N. de Sousa-Fontoura, Celso A. Camara & Tania M. S. Silva

  3. Biotério Central, Universidade Estadual do Rio Grande do Norte, Mossoró, RN, Brazil

    Kizzy M. de F. M. Costa

  4. Laboratório de Farmacognosia, Faculdade de Farmácia da Universidade de Lisboa, Lisbon, Portugal

    Rita M. O. T. Serrano & Olga M. D. Silva

Authors
  1. Déborah M. N. de Sousa-Fontoura
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  2. Roberio G. Olinda
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  3. Geysa A. Viana
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  4. Kizzy M. de F. M. Costa
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  5. Jael S. Batista
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  6. Rita M. O. T. Serrano
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  7. Olga M. D. Silva
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  8. Celso A. Camara
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  9. Tania M. S. Silva
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Contributions

DMN and TMSS performed experiments and wrote the manuscript, RGO, GAV, KMFMC, JSB, RMOTS, OMDS, and CAC interpreted data, prepared and provided the key materials. All the authors have read the final manuscript and approved the submission.

Corresponding author

Correspondence to Tania M. S. Silva.

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The authors declare that no experiments were performed on humans or animals for this study.

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The authors declare that they have followed the protocols of their work center on the publication of patient data.

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de Sousa-Fontoura, D.M.N., Olinda, R.G., Viana, G.A. et al. Wound healing activity and chemical composition of geopropolis from Melipona subnitida. Rev. Bras. Farmacogn. 30, 367–373 (2020). https://doi.org/10.1007/s43450-020-00030-8

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  • DOI: https://doi.org/10.1007/s43450-020-00030-8

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