lncRNA NR2F1-AS1 has been reported to be upregulated in hepatocellular carcinoma and plays oncogenic roles. Through the analysis of TCGA dataset, we observed the downregulation of NR2F1-AS1 in cervical squamous cell carcinoma (CSCC). This study was performed to analyze the functionality of NR2F1-AS1 in CSCC. It was observed that NR2F1-AS1 was significantly downregulated in tumor tissues than in paired non-tumor tissues in CSCC patients. Levels of NR2F1-AS1 decreased with increased in clinical stages. NR2F1-AS1 can directly interact with miR-17. Overexpression experiments showed that NR2F1-AS1 and miR-17 have no significant effects on the expression of each other. Interestingly, NR2F1-AS1 overexpression led to the upregulation of SIK1, a target of miR-17. Transwell assay analysis revealed decreased invasion and migration rates of CSCC cells after NR2F1-AS1 and SIK1 overexpression. miR-17 overexpression played an opposite role and reduced the effects of NR2F1-AS1 and SIK1 overexpression. Therefore, NR2F1-AS1 regulates miR-17/SIK1 axis to suppress the invasion and migration of CSCC cells.
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This work was financially supported by grants from the Key Talents for Maternal and Child Health of Jiangsu Province (FRC201714) and the National Natural Science pre-research project of The Second Affiliated Hospital of Soochow University (SDFEYGJ1706).
Protocol of the present study was approved by the Ethics Committee of Suzhou Wuzhong People’s Hospital and The Second Affiliated Hospital of Soochow University. All participants (60 CSCC patients, females, 39 years to 67 years, 53.1 ± 5.8 years) signed informed consent.
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Peng, J., Hou, F., Zhu, W. et al. lncRNA NR2F1-AS1 Regulates miR-17/SIK1 Axis to Suppress the Invasion and Migration of Cervical Squamous Cell Carcinoma Cells. Reprod. Sci. 27, 1534–1539 (2020). https://doi.org/10.1007/s43032-020-00149-y
- Cervical squamous cell carcinoma