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Table 3 Examples of media used to recover marine bacterial fish pathogens

From: Methods for the diagnosis of bacterial fish diseases

MediumPathogen
Non-selective medium
 Brain heart infusion agar + 3% (w/v) NaClShewanella putrefaciens
 Cytophaga agar prepared in seawaterTenacibaculum spp.
 Flexibacter maritimus medium (FMM)Tenacibaculum spp.
 Marine 2216E agarPhotobacterium damselae, Vibrio spp.
 Tryptone soya agar + 1–2% (w/v) NaClAeromonas salmonicida, Aliivibrio salmonicida, V. harveyi, V. splendidus, V. vulnificus
Non-selective medium—enriched
 AUSTRAL-Salmonid Rickettsial Septicaemia (SRS) broth + l-cysteinePiscirickettsia salmonis
 Blood agar + 0.5–1.5% (w/v) NaClAliivibrio wodanis, Moritella marina, Moritella viscosa, Photobacterium damselae subsp. piscicida, V. splendidus, V. tapetis
 Cystine heart agar supplemented with 1% (w/v) haemoglobinFrancisella spp.
 Glucose asparagine agarNocardia spp.
 Kidney disease medium 2 (KDM2)R. salmoninarum
 Löwenstein–Jensen medium/dorset egg mediumMycobacterium spp., Nocardia spp.
 Middlebrook 7H10 mediumMycobacterium spp.
 Ogawa egg mediumMycobacterium gordonae
Selective medium
 Coomassie brilliant blue agarAeromonas salmonicida
 Selective kidney disease médium (SKDM)R. salmoninarum
 Thiosulphate citrate bile salt sucrose agar (TCBS)Photobacterium damselae, Vibrio spp.