The main of this study was to evaluate the inhibitory effect on the in vitro formation of the Staphylococcus aureus biofilm formed on a polyethylene (PE) surface with a nanostructured Gold (Au) coating for medical devices. An experimental in vitro study was carried out using PE discs with an Au nanoparticle coating (AuNPs) on one side (experimental group) and without coating on the other (control group); the discs were mounted in the CDC biofilm reactor adding broth of yeast-dextrose-peptone (YPD) sterile culture inoculated with S. aureus in a cell suspension (5 × 108 cells/ml). The specimens were evaluated at different times (6, 12, 24, 48, 72 h) and stained with the Live/Dead Bacterial Viability Kit (Invitrogen) for observation, analysis, and quantification with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). The results showed that as evaluation time passed an increasing of S. aureus biofilm formation was observed in the control group, in the experimental group, a statistically significant biofilm inhibition was observed with respect to the AuNPs uncoated specimens (p ≤ 0.05) and showed a ratio of almost 4:1 viable/nonviable in the biofilm of the uncoated surfaces, with a difference > 5 Log10 in the CFU counts. The PE with AuNP coating showed an inhibitory effect on the biofilm formation of S. aureus.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Houacine C, Yousaf SS, Khan I, Khurana RK, Singh KK (2018) Potential of natural biomaterials in nano-scale drug delivery. Curr Pharm Des 24(43):5188–5206. https://doi.org/10.2174/1381612825666190118153057
Jarząb N, Walczak M, Smoliński D, Sionkowska A (2019) The impact of medicinal brines on microbial biofilm formation on inhalation equipment surfaces. Biofouling 34(9):963–975. https://doi.org/10.1080/08927014.2018.1515353
Hempel JM (2015) Single-stage ear reconstruction and hearing restoration using polyethylene implant and implantable hearing devices. Facial Plast Surg 31(6):604–610. https://doi.org/10.1055/s-0035-1567887
Capek I (2017) Polymer decorated gold nanoparticles in nanomedicine conjugates. Adv Colloid Interf Sci 249:386–399. https://doi.org/10.1016/j.cis.2017.01.007
Hamouda IM (2012) Current perspectives of nanoparticles in medical and dental biomaterials. J Biomed Res 26(3):143–151. https://doi.org/10.7555/JBR.26.20120027
Ahiwale SS, Bankar AV, Tagunde SBP, Kapadnis (2017) A bacteriophage mediated gold nanoparticles synthesis and their antibiofilm activity. Indian J Microbiol 57(2):188–194. https://doi.org/10.1007/s12088-017-0640-x
Hu D, Li H, Wang B, Ye Z, Lei W, Jia F, Jin Q, Ren KF, Ji J (2017) Surface-adaptive gold nanoparticles with effective adherence and enhanced photothermal ablation of methicillin-resistant staphylococcus aureus biofilm. ACS Nano 11(9):9330–9339. https://doi.org/10.1021/acsnano.7b04731
Donlan RM (2002) Biofilms: microbial life on surfaces. Emerg Infect Dis 8(9):881–890. https://doi.org/10.3201/eid0809.020063
Azeredo J, Azevedo NF, Briandet R, Cerca N, Coenye T, Costa AR, Desvaux M, di Bonaventura G, Hébraud M, Jaglic Z, Kačániová M, Knøchel S, Lourenço A, Mergulhão F, Meyer RL, Nychas G, Simões M, Tresse O, Sternberg C (2017) Critical review on biofilm methods. Crit Rev Microbiol 43(3):313–351. https://doi.org/10.1080/1040841X.2016.1208146
Goeres DM, Loetterle LR, Hamilton MA, Murga R, Kirby DW, Donlan RM (2015) Statistical assessment of a laboratory method for growing biofilms. Microbiology 151(Pt 3):757–762. https://doi.org/10.1099/mic.0.27709-0
Percival SL, Suleman L, Vuotto C, Donelli G (2015) Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control. J Med Microbiol 64(Pt 4):323–334. https://doi.org/10.1099/jmm.0.000032
Moormeier DE, Bayles KW (2017) Staphylococcus aureus biofilm: a complex developmental organism. Mol Microbiol 104(3):365–376. https://doi.org/10.1111/mmi.13634
Kennedy CA, O’Gara JP (2004) Contribution of culture media and chemical properties of polystyrene tissue culture plates to biofilm development by Staphylococcus aureus. J Med Microbiol 53(Pt 11):1171–1173. https://doi.org/10.1099/jmm.0.45764-0
Priya MRK, Lyer PR (2015) Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines. Appl Nanosci 5:443–448. https://doi.org/10.1007/s13204-014-0336-z
Rai A, Prabhune A, Perry AA (2010) Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings. J Mater Chem 20:6789–6798. https://doi.org/10.1039/C0JM00817F
Prabhawathiac V, Malliappan PS, Boobalana T, Manohara CM, Doblea M (2019) Design of antimicrobial polycaprolactam nanocomposite by immobilizing T subtilisin conjugated Au/Ag core-shell nanoparticles for biomedical applications. Mater Sci Eng C Mater Biol Appl 94:656–665. https://doi.org/10.1016/j.msec.2018.10.020
Lu B, Lu F, Ran L, Yu K, Xiao Y, Li Z, Dai F, Wu D, Lan G (2018) Imidazole-molecule-capped chitosan-gold nanocomposites with enhanced antimicrobial activity for treating biofilm-related infections. J Colloid Interface Sci 531:269–281. https://doi.org/10.1016/j.jcis.2018.07.058
Sathyanarayanan MB, Balachandranath R, Genji Srinivasulu Y, Kannaiyan SK, Subbiahdoss G (2013) The effect of gold and iron-oxide nanoparticles on biofilm-forming pathogens. SRN Microbiol 25:272086. https://doi.org/10.1155/2013/272086
Yu Q, Li J, Zhang Y, Wang Y, Liu L, Li M (2016) Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells. Sci Rep 6:26667. https://doi.org/10.1038/srep26667
Boda SK, Broda J, Schiefer F, Weber-Heynemann J, Hoss M, Simon U, Basu B, Jahnen-Dechent W (2015) Cytotoxicity of ultrasmall gold nanoparticles on planktonic and biofilm encapsulated gram-positive Staphylococci. Small 11(26):3183–3193. https://doi.org/10.1002/smll.201403014
Roy R, Tiwari M, Donelli G, Tiwari V (2018) Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence 9(1):522–554. https://doi.org/10.1080/21505594.2017.1313372
Xie Y, Liu Y, Yang J (2018) Gold nanoclusters for targeting MRSA in vivo. Angew Chem Int Ed Eng 57(15):3958–3962. https://doi.org/10.1002/anie.201712878
Jain PK, Huang X, El-Sayed IH, El-Sayed MA (2008) Noble metals on the nanoscale: optical and photothermal properties and some applications in imaging, sensing, biology and medicine. Acc Chem Res 41(12):1578–1586. https://doi.org/10.1021/ar7002804
Yougbare S, Chang TK, Tan SH, Kuo JC, Hsu PH, Su CY et al (2019) Antimicrobial gold nanoclusters: recent developments and future perspectives. Int J Mol Sci 20(12):2924. https://doi.org/10.3390/ijms20122924
Lomelí-Marroquín D, Medina Cruz D, Nieto-Argüello A, Vernet Crua A, Chen J, Torres-Castro A et al (2019) Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents. Int J Nanomedicine 14:2171–2190. https://doi.org/10.2147/IJN.S192757
Peña-González CE, Pedziwiatr-Werbicka E, Martín-Pérez T, Szewczyk EM, Copa-Patiño JL et al (2017) Antibacterial and antifungal properties of dendronized silver and gold nanoparticles with cationic carbosilane dendrons. Int J Pharm 528(1-2):55–61. https://doi.org/10.1016/j.ijpharm.2017.05.067
Bing W, Sun H, Wang F, Song Y, Ren J (2018) Hydrogen-producing hyperthermophilic bacteria synthesized size-controllable fine gold nanoparticles with excellence for eradicating biofilm and antibacterial applications. J Mater Chem B 6(28):4602–4609. https://doi.org/10.1039/c8tb00549d
Marco Antonio Álvarez-Pérez want to thanks to the financial support by the Dirección General de Personal Academico-Universidad Nacional Autonoma de Mexico: PAPIIT IT203618 project.
Conflicts of interest/competing interests
All the authors declare that they have no conflict of interest.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Responsible Editor: Fernando R. Pavan.
About this article
Cite this article
Villa-García, L.D., Márquez-Preciado, R., Ortiz-Magdaleno, M. et al. Antimicrobial effect of gold nanoparticles in the formation of the Staphylococcus aureus biofilm on a polyethylene surface. Braz J Microbiol 52, 619–625 (2021). https://doi.org/10.1007/s42770-021-00455-w
- Staphylococcus aureus,
- Gold nanoparticles,
- Biofilm reactor