Production and characterization of poly 3-hydroxybutyrate-co-3-hydroxyvalerate in wheat starch wastewater and its potential for nanoparticle synthesis

Abstract

Polyhydroxyalkanoates (PHAs) are polymers with biodegradable and biocompatible properties accumulated in a wide variety of bacterial strains. In the present study, active sludge, wheat starch wastewater (WSW), and oil wastewater were used for the isolation and screening of PHA-accumulating bacteria. WSW was then implemented as a cheap and economical culture medium for the production of PHAs by the selected isolate. The extracted PHA was characterized, and the capability of produced biopolymer for preparing nanoparticles was evaluated. Based on the results, 96 different bacterial isolates were obtained, of which the strains isolated from WSW demonstrated the highest PHA-accumulation capability. The maximum PHA content of 3.07 g/l (59.50% of dry cell weight) was obtained by strain N6 in 21 h. The selected strain was identified by molecular approaches as Bacillus cereus. Afterward, the physicochemical characterization of an accumulated biopolymer was specified as a PHBV copolymer. Finally, spherical homogenous PHBV nanoparticles with a size of 137 nm were achieved. The PHBV nanoparticles showed a suitable small size and good zeta potential for medical applications. Hence, it can be concluded that isolated wild strain (B. cereus) has the potential exploitation capability for cost-effective PHBV production using the WSW.

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Data availability

All isolated strains are preserved in Persian Type Culture Collection (PTCC) and data are available by N. Sinaei.

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Acknowledgments

The authors are thankful to the Commonwealth Scientific and Industrial Research Organization (CSIRO), Australia, for providing laboratory facilities for nanoparticle study. We are also grateful to Ms. Mohseni, the head of the Persian Type Culture Collection (PTCC), for identifying the isolated strains.

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Design of study was accomplished by Davood Zare, Neda Sinaei, and Mehrdad Azin. Data and draft of manuscript were collected by Neda Sinaei and finalized by Davood Zare and Mehrdad Azin. All authors read and approved the final manuscript.

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Correspondence to Davood Zare.

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Sinaei, N., Zare, D. & Azin, M. Production and characterization of poly 3-hydroxybutyrate-co-3-hydroxyvalerate in wheat starch wastewater and its potential for nanoparticle synthesis. Braz J Microbiol (2021). https://doi.org/10.1007/s42770-021-00430-5

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Keywords

  • Polyhydroxyalkanoates
  • Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)
  • Wheat starch wastewater
  • Bacillus cereus
  • Nanoparticle
  • Medical application