First reported case of Gilbertella persicaria in human stool: outcome of a community study from Segamat, Johor, Malaysia

Abstract

Species of fungi belonging to the order Mucorales can be found everywhere in the environment. Gilbertella persicaria, which belongs to this order, have often been isolated from fruits and in water systems. However, there has been no report of isolation of this fungus from human samples. During a gut mycobiome study, from the Segamat community, Gilbertella persicaria was isolated from a human fecal sample and was characterized through a series of morphological assessment, biochemical tests, and molecular techniques. The isolate produced a white velvety surface that turned grayish after 24 h. Although no biofilm production was observed, the results indicated that the isolate could form calcium oxalate crystals, produced urease, and was resistant to low pH. The isolate was sensitive to amphotericin but resistant to voriconazole and itraconazole. The features of this fungus that could help in its survival in the human gut are also discussed.

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Acknowledgments

The authors wish to thank Monash University Malaysia, Tropical Medicine and Biology Multidisciplinary Platform and the South East Asia Community Observatory for their support.

Funding

This work was supported by Monash University Malaysia multidisciplinary project funding [LG-2017-01-SCI], Monash University Australia funding [SCI/MUA/02–2019/001], the School of Science Monash University Malaysia, and discretionary funding from Tropical Medicine and Biology Multidisciplinary Platform.

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Correspondence to Joash Ban Lee Tan.

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Ethical approval was given by the Monash University Human Research Ethics Committee (MUHREC, Project ID/Approval no.: 1516) which is per the WMA Declaration of Helsinki [16] and complied with international and institutional standards.

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Huët, M.A.L., Wong, L.W., Goh, C.B.S. et al. First reported case of Gilbertella persicaria in human stool: outcome of a community study from Segamat, Johor, Malaysia. Braz J Microbiol (2020). https://doi.org/10.1007/s42770-020-00323-z

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Keywords

  • Mucorales
  • Gilbertella persicaria
  • Human gut
  • Fecal sample
  • Malaysia