Decoding social behaviors in a glycerol dependent bacterial consortium during Reactive Blue 28 degradation

Abstract

Biodegradation of reactive azo dyes has been an arduous problem for decades. Several efficient biosystems have been proposed for dye degradation, but most of them are dependent on the availability of costly co-substrates such as peptone, yeast extract, and/or glucose. The present study describes the azo dye degradation by a bacterial consortium using glycerol as a sole co-substrate. The consortium was developed from a mixed bacterial culture obtained upon enrichment of soil sediment for Reactive Blue 28 (RB28) decolorization in the presence of glycerol (0.1%; v/v). The consortium with three bacterial species, i.e., Stenotrophomonas acidaminiphila APG1, Cellulomonas sp. APG4, and Pseudomonas stutzeri APG2, designated as “SCP,” decolorized 92% of 100 ppm dye in 96 h. The intricacies of the interactions existing within the members of the consortium were resolved by a simple and unique analysis called “BSocial.” Among all the members, Cellulomonas sp. APG4 exerted a net-positive impact for decolorization (%) on the consortium. The net fitness of the community increased when all the three species were present, and thus, all of them were selected for further analysis. Moreover, APG4 seemed to be central in the reductive decolorization as it possessed the highest reductase activity. The dye degradation by the consortium was demonstrated by UV-Visible spectroscopy, HPTLC, and FTIR spectroscopy of control and decolorized cell-free supernatant. The LC-ESI-MS analysis of metabolites extracted from decolorized cell-free medium led to the identification of degradation products, thus leading us to propose the plausible pathway for degradation of RB28 by bacterial consortium.

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Acknowledgments

The authors are also grateful to CISST, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India, for providing analytical facilities. Sandhya Nanjani gratefully acknowledges the University Grants Commission, New Delhi, for the award of SRF to pursue doctoral studies.

Funding

The authors acknowledge the funds received from the Department of Biotechnology-Centre of Excellence and Innovation in Biotechnology (DBT-CEIB) and Department of Science and Technology-Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (DST-FIST) Program, Ministry of Science and Technology, and CAS program funded by UGC, New Delhi, India.

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Nanjani, S., Rawal, K. & Keharia, H. Decoding social behaviors in a glycerol dependent bacterial consortium during Reactive Blue 28 degradation. Braz J Microbiol (2020). https://doi.org/10.1007/s42770-020-00303-3

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Keywords

  • Consortium
  • Glycerol
  • BSocial
  • Microbial interaction
  • Mass spectrometry
  • Azo dye decolorization