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Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test

  • Clinical Microbiology - Research Paper
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A Correction to this article was published on 14 March 2019

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Abstract

Sporotrichosis is an infection of the skin caused by traumatic inoculation of the fungus Sporothrix schenckii. Definitive diagnosis relies on direct visualization of the fungus or its isolation on culture medium, although both have low sensitivity. Alternatively, the detection of the antibody response offers a more rapid alternative for diagnosis. Although the available immunoassays possess good sensitivity and specificity, cross-reactivity is still a problem. This study aimed to evaluate the effect of sodium metaperiodate and 6 M urea solutions on the serological diagnosis of sporotrichosis using an enzyme-linked immunosorbent assay (ELISA) test. Ninety-six-well plates were sensitized with exoantigens from the yeast phase of S. schenckii. Sera of patients with confirmed sporotrichosis, sera of patients with paracoccidioidomycosis, and sera of individuals with a sporotrichin-negative skin test were tested. Two strategies were used; the first consisted of treating the antigen with sodium metaperiodate solution for different incubation times, and the second consisted of treating the serum with 6 M urea solution for different incubation times. ROC curve analysis revealed that the best discrimination parameters were obtained using 6 M urea solution incubated for 5 min and serum dilution at 1/600. The use of 6 M urea solution improves the performance of the ELISA test in the diagnosis of sporotrichosis.

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Change history

  • 14 March 2019

    In the article mentioned above an author’s name was misspelled. The correct author name reads as follows: Leila Maria Lopes-Bezerra. We apologize for the inconvenience.

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Acknowledgments

We thank Prof. Dr. Mario Leon Silva Vergara from Universidade Federal do Triângulo Mineiro (UFTM) for providing sera and information about the medical status of the patients included in this study. We also thank Dr. Rosane Orofino Costa of Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil for her clinical contribution for the sera collection supported by Faperj/PPSUS project. Also, we thank Dr. Felipe Fornias Sperandio and Dr. Anderson Messias Rodrigues for their critical review of the manuscript and Dr. Jorge Kleber Chavasco in the production of rabbit antibodies against S. schenckii antigens.

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Authors and Affiliations

  1. Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700 – Centro, Alfenas, MG, CEP 37130-001, Brazil

    Letícia Maria Leomil Coelho, Julianne Caravita Grisolia, Jorge Kleber Chavasco, Luiz Felipe Leomil Coelho & Luiz Cosme Cotta Malaquias

  2. Universidade José do Rosário Vellano, Alfenas, MG, Brazil

    Maria Lúcia & Míriam Boczar

  3. Instituto de Ciências Exatas, Universidade Federal de Alfenas, Alfenas, MG, Brazil

    Eric Batista Ferreira & Denismar Alves Nogueira

  4. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil

    Zoilo Pires de Camargo

  5. Laboratório de Micologia Celular e Proteômica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Leila Maria Lopes Bezerra

Authors
  1. Letícia Maria Leomil Coelho
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  2. Julianne Caravita Grisolia
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  9. Leila Maria Lopes Bezerra
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  10. Luiz Felipe Leomil Coelho
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  11. Luiz Cosme Cotta Malaquias
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Correspondence to Luiz Cosme Cotta Malaquias.

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Coelho, L.M.L., Grisolia, J.C., Lúcia, M. et al. Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test. Braz J Microbiol 50, 139–145 (2019). https://doi.org/10.1007/s42770-018-0005-6

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  • DOI: https://doi.org/10.1007/s42770-018-0005-6

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