Skip to main content
SpringerLink
Log in

Multifunctional and Sprayable 2D MoS2/Silk Sericin Bio-Nanocomposite Dressings with Enhanced Photothermal Effect for Infected Wound Healing

  • Research Article
  • Published:
Advanced Fiber Materials Aims and scope Submit manuscript

Abstract

Developing novel antibacterial dressing protecting skin injuries from infection is essential for wound healing. In this study, sericin, a bio-waste produced during the degumming of silk cocoons, is utilized to exfoliate MoS2 layers and improve the dispersity and stability of MoS2 nanosheets (MoS2-NSs). Moreover, owing to its ability to promote oxygen permeability and cell growth and its good biocompatibility, MoS2-NS/Sericin maintains its photothermal property under an 808 nm light source for a strong antibacterial activity as well as improves the fibroblast migration, which accelerates wound healing. Furthermore, the in vitro experiments indicates that MoS2-NS/Sericin can also scavenge reactive oxygen species (ROS) at an inflammatory stage of wound healing and transform classical activated macrophages (M1-type) into alternatively activated macrophages (M2-type), which is beneficial for wound recovery. Based on these results observed in vitro, full-thickness skin wound experiments are conducted on rats, and the corresponding results show that MoS2/Sericin under 808 nm irradiation exhibits the best performance in promoting wound healing. Overall, MoS2-NS/Sericin exhibits a high potential for bacteria-infected wound healing.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

References

  1. Zhao Y, Wang DD, Qian TW, Zhang JM, Li ZH, Gong QY, Ren XZ, Zhao YL. Biomimetic nanozyme-decorated hydrogels with H2O2-activated oxygenation for modulating immune microenvironment in diabetic wound. ACS Nano. 2023;17:16854.

    Article  CAS  PubMed  Google Scholar 

  2. Wang LS, Duan L, Liu G, Sun JF, Shahbazi MA, Kundu SC, Reis RL, Xiao B, Yang X. Bioinspired polyacrylic acid-based dressing: Wet adhesive, self-healing, and multi-biofunctional coacervate hydrogel accelerates wound healing. Adv Sci. 2023;10:e2207352.

    Article  Google Scholar 

  3. White MJV, Briquez PS, White DAV, Hubbell JA. VEGF-A, PDGF-BB and HB-EGF engineered for promiscuous super affinity to the extracellular matrix improve wound healing in a model of type 1 diabetes. npj Regener Med. 2021;6:76.

    Article  CAS  Google Scholar 

  4. Wang M, Wang CG, Chen M, Xi YW, Cheng W, Mao C, Xu TZ, Zhang XX, Lin C, Gao WY, Guo Y, Lei B. Efficient angiogenesis-based diabetic wound healing/skin reconstruction through bioactive antibacterial adhesive ultraviolet shielding nanodressing with exosome release. ACS Nano. 2019;13:10279.

    Article  CAS  PubMed  Google Scholar 

  5. Yi SX, Zhou Y, Zhang JM, Wang M, Zheng SH, Yang X, Duan L, Reis RL, Dai FY, Kundu SC, Xiao B. Flat silk cocoon-based dressing: Daylight-driven rechargeable antibacterial membranes accelerate infected wound healing. Adv Healthcare Mater. 2022;11: e2201397.

    Article  Google Scholar 

  6. Wang CF, Luo Y, Liu XM, Cui ZD, Zheng YF, Liang YQ, Li ZY, Zhu SL, Lei J, Feng XB, Wu SL. The enhanced photocatalytic sterilization of MOF-Based nanohybrid for rapid and portable therapy of bacteria-infected open wounds. Bioact Mater. 2022;13:200.

    PubMed  Google Scholar 

  7. Yi SX, Wu YH, Zhang YS, Zou YS, Dai FY, Si Y. Antibacterial activity of photoactive silk fibroin/cellulose acetate blend nanofibrous membranes against Escherichia coli. ACS Sustain Chem Eng. 2020;8:16775.

    Article  CAS  Google Scholar 

  8. Wang XL, Li QL, Miao Y, Chen XQ, Zhang XY, Shi JR, Liu F, Wang XQ, Li ZH, Yang YX, Zhang XY, Wang JL, Duan JY. A 0D–2D heterojunction bismuth molybdate-anchored multifunctional hydrogel for highly efficient eradication of drug-resistant bacteria. ACS Nano. 2023;17:15568.

    Article  CAS  PubMed  Google Scholar 

  9. Xi YW, Ge J, Guo Y, Lei B, Ma PX. Biomimetic elastomeric polypeptide-based nanofibrous matrix for overcoming multidrug-resistant bacteria and enhancing full-thickness wound healing/skin regeneration. ACS Nano. 2018;12:10772.

    Article  CAS  PubMed  Google Scholar 

  10. Yang YT, Du YZ, Zhang J, Zhang HL, Guo BL. Structural and functional design of electrospun nanofibers for hemostasis and wound healing. Adv Fiber Mater. 2022;4:1027.

    Article  CAS  Google Scholar 

  11. Chhabra J, Chopra H, Pahwa R, Raina N, Wadhwa K, Saini S, Negi P, Gupta M, Singh I, Dureja H, Emran TB. Potential of nanoemulsions for accelerated wound healing: Innovative strategies. NT J Surg. 2023;109:2365.

    Google Scholar 

  12. Yu R, Zhang HL, Guo BL. Conductive biomaterials as bioactive wound dressing for wound healing and skin tissue engineering. Nano-Micro Lett. 2021;14:1.

    Article  Google Scholar 

  13. Zhang JY, Chen HL, Zhao M, Liu GT, Wu J. 2D nanomaterials for tissue engineering application. Nano Res. 2019;2020:13.

    Google Scholar 

  14. Peng GT, Fadeel B. Understanding the bidirectional interactions between two-dimensional materials, microorganisms, and the immune system. Adv Drug Deliv Rev. 2022;188: 114422.

    Article  CAS  PubMed  Google Scholar 

  15. Feng XB, Ma L, Lei J, Ouyang QL, Zeng YX, Luo Y, Zhang XG, Song Y, Li GC, Tan L, Liu XM, Yang C. Piezo-augmented sonosensitizer with strong ultrasound-propelling ability for efficient treatment of osteomyelitis. ACS Nano. 2022;16:2546.

    Article  CAS  PubMed  Google Scholar 

  16. Li H, Gong MH, Xiao JY, Hai L, Luo YZ, He LD, Wang ZF, Deng L, He DG. Photothermally activated multifunctional MoS2 bactericidal nanoplatform for combined chemo/photothermal/photodynamic triple-mode therapy of bacterial and biofilm infections. Chem Eng J. 2022;429:132600.

    Article  CAS  Google Scholar 

  17. Zhang XY, Zhang C, Yang YQ, Zhang HY, Huang XB, Hang RQ, Yao XH. Light-assisted rapid sterilization by a hydrogel incorporated with Ag3PO4/MoS2 composites for efficient wound disinfection. Chem Eng J. 2019;374:596.

    Article  CAS  Google Scholar 

  18. Chen JW, Qiu LP, Li QL, Ai J, Liu HQ, Chen QH. Rapid hemostasis accompanied by antibacterial action of calcium crosslinking tannic acid-coated mesoporous silica/silver Janus nanoparticles. Mater Sci Eng C-Mater. 2021;123: 111958.

    Article  CAS  Google Scholar 

  19. Zhang XY, Zhang GN, Zhang HY, Liu XP, Shi J, Shi HX, Yao XH, Chu PK, Zhang XY. A bifunctional hydrogel incorporated with CuS@MoS2 microspheres for disinfection and improved wound healing. Chem Eng J. 2020;382:122849.

    Article  CAS  Google Scholar 

  20. He JH, Li ZL, Wang JX, Li TY, Chen JY, Duan XL, Guo BL. Photothermal antibacterial antioxidant conductive self-healing hydrogel with nitric oxide release accelerates diabetic wound healing. Compos Part B-Eng. 2023;266:110985.

    Article  CAS  Google Scholar 

  21. Yang YT, Li M, Pan GY, Chen JY, Guo BL. Multiple stimuli-responsive nanozyme-based cryogels with controlled NO release as self-adaptive wound dressing for infected wound healing. Adv Funct Mater. 2023;33:19.

    Google Scholar 

  22. Yin WY, Yu J, Lv FT, Yan L, Zheng LR, Gu ZJ, Zhao YL. Functionalized nano-MoS2 with peroxidase catalytic and near-infrared photothermal activities for safe and synergetic wound antibacterial applications. ACS Nano. 2016;10:11000.

    Article  CAS  PubMed  Google Scholar 

  23. Yang ZX, Chen CH, Li B, Zheng YF, Liu XM, Shen J, Zhang Y, Wu SL. A core–shell 2D-MoS2@MOF heterostructure for rapid therapy of bacteria-infected wounds by enhanced photocatalysis. Chem Eng J. 2023;451:139127.

    Article  CAS  Google Scholar 

  24. Wang GT, Wang T, Dang Y, Lu ZW, Su GH, Feng B, Zhuo Y, Jiang XM, Ye QB, Wu C, Pu X, Zhao Y, Zhao XQ, Cai S, Du SY, Jia SS, Wang YY, Wu D, Rao HB, Sun MM. Insights into the antibacterial mechanism of MoS2/CoS2 heterostructure nanozymes with double enzyme-like activities for MRSA-infected wound therapy. Chem Eng J. 2023;461:141959.

    Article  CAS  Google Scholar 

  25. Li H, Wu JMT, Yin ZY, Zhang H. Preparation and applications of mechanically exfoliated single-layer and multilayer MoS2 and WSe2 nanosheets. Acc Chem Res. 2014;47:1067.

    Article  CAS  PubMed  Google Scholar 

  26. Li Y, Fu RZ, Duan ZG, Zhu CH, Fan DD. Construction of multifunctional hydrogel based on the tannic acid-metal coating decorated MoS2 dual nanozyme for bacteria-infected wound healing. Bioact Mater. 2022;9:461.

    CAS  PubMed  Google Scholar 

  27. Liu J, Deng Y, Fu DA, Yuan Y, Li QL, Shi L, Wang GB, Wang Z, Wang L. Sericin microparticles enveloped with metal-organic networks as a pulmonary targeting delivery system for intra-tracheally treating metastatic lung cancer. Bioact Mater. 2021;6:273.

    CAS  PubMed  Google Scholar 

  28. Lamboni L, Gauthier M, Yang G, Wang Q. Silk sericin: a versatile material for tissue engineering and drug delivery. Biotechnol Adv. 1855;2015:33.

    Google Scholar 

  29. Ma Y, Duan L, Sun JF, Gou SQ, Chen FY, Liang YQ, Dai FY, Xiao B. Oral nanotherapeutics based on Antheraea pernyi silk fibroin for synergistic treatment of ulcerative colitis. Biomaterials. 2022;282:121410.

    Article  CAS  PubMed  Google Scholar 

  30. Tao G, Wang YJ, Cai R, Chang HP, Song K, Zuo H, Zhao P, Xia QY, He HW. Design and performance of sericin/poly(vinyl alcohol) hydrogel as a drug delivery carrier for potential wound dressing application. Mat Sci Eng C-Mater. 2019;101:341.

    Article  CAS  Google Scholar 

  31. Chouhan D, Mandal BB. Silk biomaterials in wound healing and skin regeneration therapeutics: from bench to bedside. Acta Biomater. 2020;103:24.

    Article  CAS  PubMed  Google Scholar 

  32. Ma Y, Zhou L, Yang CH, Wang LS, Yi SX, Tong XL, Xiao B, Chen JC. Comparison of sericins from different sources as natural therapeutics against ulcerative colitis. ACS Biomater Sci Eng. 2021;7:4626.

    Article  CAS  PubMed  Google Scholar 

  33. Kathiravan D, Huang BR, Saravanan A, Prasannan A, Hong PD. Highly enhanced hydrogen sensing properties of sericin-induced exfoliated MoS2 nanosheets at room temperature. Sensor Actuat B-Chem. 2019;279:138.

    Article  CAS  Google Scholar 

  34. Clark SJ, Segall MD, Pickard CJ, Hasnip PJ, Probert MJ, Refson K, Payne MC. First principles methods using castep. Z Krist-Cryst Mater. 2005;220:567.

    Article  CAS  Google Scholar 

  35. Perdew JP, Burke K, Ernzerhof M. Generalized gradient approximation made simple. Phys Rev Lett. 1996;77:3865.

    Article  CAS  PubMed  Google Scholar 

  36. Perdew JP, Chevary JA, Vosko SH, Jackson KA, Pederson MR, Singh DJ, Fiolhais C. Atoms, molecules, solids, and surfaces: applications of the generalized gradient approximation for exchange and correlation. Phys Rev B. 1992;46:6671.

    Article  CAS  Google Scholar 

  37. Zhang T, Sun H, Wang FD, Zhang WD, Tang SW, Ma JM, Gong HW, Zhang JP. Adsorption of phosgene molecule on the transition metal-doped graphene: first principles calculations. Appl Surf Sci. 2017;425:340.

    Article  CAS  Google Scholar 

  38. Salimi M, Shokrgozar MA, Hamid DH, Vossoughi M. Photothermal properties of two-dimensional molybdenum disulfide (MoS2) with nanoflower and nanosheet morphology. Mater Res Bull. 2022;152:11837.

    Article  Google Scholar 

  39. Xu XY, Wu JY, Meng ZH, Li YR, Huang QL, Qi Y, Liu YF, Zhan D, Liu XY. Enhanced exfoliation of biocompatible MoS2 nanosheets by wool keratin. ACS Appl Nano Mater. 2018;1:5460.

    Article  CAS  Google Scholar 

  40. Coleman JN, Lotya M, O’Neill A, Bergin SD, King PJ, Khan U, Young K, Gaucher A, De S, Smith RJ, Shvets IV, Arora SK, Stanton G, Kim HY, Lee K, Kim GT, Duesberg GS, Hallam T, Boland JJ, Wang JJ, Donegan JF, Grunlan JC, Moriarty G, Shmeliov A, Nicholls RJ, Perkins JM, Grieveson EM, Theuwissen K, McComb DW, Nellist PD, Nicolosi V. Two-dimensional nanosheets produced by liquid exfoliation of layered materials. Science. 2011;331:568.

    Article  CAS  PubMed  Google Scholar 

  41. Park CJ, Ryoo J, Ki CS, Kim JW, Kim IS, Bae DG, Um IC. Effect of molecular weight on the structure and mechanical properties of silk sericin gel, film, and sponge. Int J Biol Macromol. 2018;119:821.

    Article  CAS  PubMed  Google Scholar 

  42. Vulpe R, Popa M, Picton L, Balan V, Dulong V, Butnaru M, Verestiuc L. Crosslinked hydrogels based on biological macromolecules with potential use in skin tissue engineering. Int J Biol Macromol. 2016;84:174.

    Article  CAS  PubMed  Google Scholar 

  43. Xia ZB, Liu GZ, Dong YQ, Zhang YH. Anticorrosive epoxy coatings based on polydopamine modified molybdenum disulfide. Prog Org Coat. 2019;133:154.

    Article  CAS  Google Scholar 

  44. Wang M, Pang Y, Liu DY, Zheng SH, Song QL. Tuning magnetism by strain and external electric field in zigzag Janus MoSSe nanoribbons. Comput Mater Sci. 2018;146:240.

    Article  CAS  Google Scholar 

  45. Luo YF, Pang Y, Tang M, Song QL, Wang M. Electronic properties of Janus MoSSe nanotubes. Comput Mater Sci. 2019;156:315.

    Article  CAS  Google Scholar 

  46. Chou SS, Kaehr B, Kim J, Foley BM, De M, Hopkins PE, Huang J, Brinker CJ, Dravid VP. Chemically exfoliated MoS2 as near-infrared photothermal agents. Angew Chem Int Edit. 2013;52:4160.

    Article  CAS  Google Scholar 

  47. Yan PF, Li MY, Liu J, Hu YD, Tang KY. MoS2@PDA@Ag/PVA hybrid hydrogel with excellent light-responsive antibacterial activity and enhanced mechanical properties for wound dressing. Macromol Mater Eng. 2022. https://doi.org/10.1002/mame.202100654.

    Article  Google Scholar 

  48. Teo WZ, Chng ELK, Sofer Z, Pumera M. Cytotoxicity of exfoliated transition-metal dichalcogenides (MoS2, WS2, and WSe2) is lower than that of graphene and its analogues. Chem-Eur J. 2014;20:9627.

    Article  CAS  PubMed  Google Scholar 

  49. Li YN, Zhang ZB, Kim HS, Han S, Kim SW. CD31+ cell transplantation promotes recovery from peripheral neuropathy. Mol Cell Neurosci. 2014;62:60.

    Article  CAS  PubMed  Google Scholar 

  50. Bari E, Perteghella S, Faragò S, Torre ML. Association of silk sericin and platelet lysate: premises for the formulation of wound healing active medications. Int J Biol Macromol. 2018;119:37.

    Article  CAS  PubMed  Google Scholar 

  51. Bakadia BM, Qaed Ahmed AAQ, Lamboni L, Shi ZJ, Mukole BM, Zheng RZ, Mbang MP, Zhang B, Gauthier M, Yang G. Engineering homologous platelet-rich plasma, platelet-rich plasma-derived exosomes, and mesenchymal stem cell-derived exosomes-based dual-crosslinked hydrogels as bioactive diabetic wound dressings. Bioact Mater. 2023;28:74.

    CAS  PubMed  PubMed Central  Google Scholar 

  52. Lu YZ, Jia CL, Gong CC, Wang H, Xiao Q, Guo JX, Ni DL, Xu N. A hydrogel system containing molybdenum-based nanomaterials for wound healing. Nano Res. 2023;16:5368.

    Article  CAS  Google Scholar 

  53. Zhao H, Huang J, Li Y, Lv XJ, Zhou HT, Wang HR, Xu YY, Wang C, Wang J, Liu Z. ROS-scavenging hydrogel to promote healing of bacteria infected diabetic wounds. Biomaterials. 2020;258: 120286.

    Article  CAS  PubMed  Google Scholar 

  54. Wu MH, Liu HF, Zhu YF, Chen FX, Chen Z, Guo LY, Wu P, Li GL, Zhang C, Wei RX, Cai L. Mild photothermal-stimulation based on injectable and photocurable hydrogels orchestrates immunomodulation and osteogenesis for high-performance bone regeneration. Small. 2023;19: e2300111.

    Article  PubMed  Google Scholar 

  55. Zu MH, Xie DC, Canup BSB, Chen NX, Wang YJ, Sun RX, Zhang Z, Fu YM, Dai FY, Xiao B. “Green” nanotherapeutics from tea leaves for orally targeted prevention and alleviation of colon diseases. Biomaterials. 2021;279: 121178.

    Article  CAS  PubMed  Google Scholar 

  56. Liang YQ, Li ZL, Huang Y, Yu R, Guo BL. Dual-dynamic-bond cross-linked antibacterial adhesive hydrogel sealants with on-demand removability for post-wound-closure and infected wound healing. ACS Nano. 2021;15:7078.

    Article  CAS  PubMed  Google Scholar 

  57. Cui TT, Yu JF, Wang CF, Chen S, Li Q, Guo K, Qing RK, Wang GF, Ren JA. Micro-gel ensembles for accelerated healing of chronic wound via pH regulation. Adv Sci. 2022;9: e2201254.

    Article  Google Scholar 

  58. Vedakumari SW, Veda Jancy SJV, Pravin YR, Bhoopathy J, Iyswariya K, Thomas S, Rubiya R, Prabakaran L, Kumar C, Prabu P, Murugesan R. Facile synthesis of sericin modified graphene oxide nanocomposites for treating ischemic diseases. Environ Res. 2022;209: 112925.

    Article  CAS  PubMed  Google Scholar 

  59. Hayta SB, Durmus K, Altuntas EE, Yildiz E, Hisarciklio M, Akyol M. The reduction in inflammation and impairment in wound healing by using strontium chloride hexahydrate. Cutan Ocul Toxicol. 2018;37:24.

    Article  Google Scholar 

  60. Yang Z, Min ZJ, Yu B. Reactive oxygen species and immune regulation. Int Rev Immunol. 2020;39:292.

    Article  CAS  PubMed  Google Scholar 

  61. Fox JD, Baquerizo-Nole KL, Keegan BR, Macquhae F, Escandon J, Espinosa A, Perez C, Romanelli P, Kirsner RS. Adalimumab treatment leads to reduction of tissue tumor necrosis factor-alpha correlated with venous leg ulcer improvement: a pilot study. Int Wound J. 2016;13:963.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 22008201), Natural Science Foundation Innovation and Development Joint Fund Project of Chongqing (No. CSTB2023NSCQ-LZX0028), Fundamental Research Funds for the Central Universities (No. SWU-KW22004), Special Program Project (No. 2022-JCJQ-ZD-224-12) and Open Project Program of the Ministry of Education of the Key Laboratory of Textile Fiber and Products (No. Fzxw2021001).

Author information

Author notes

  1. Libin Qiu and Lian Duan contributed equally to the work.

Authors and Affiliations

  1. State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, 400715, People’s Republic of China

    Libin Qiu, Lian Duan, Hongyu Lin, Guilong Peng, Xiao Yang & Shixiong Yi

  2. Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing, 400715, People’s Republic of China

    Min Wang

  3. State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Huaping Liang

  4. Innovation Center for Textile Science and Technology, Donghua University, Shanghai, 200051, People’s Republic of China

    Yang Si

Authors
  1. Libin Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lian Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongyu Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Min Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Huaping Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Guilong Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiao Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yang Si
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shixiong Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

XY, YS, and SY conceived and designed the study. LQ and LD wrote the manuscript. LQ, LD, and HL organized the data. MW, HL and GP supervised the project. #LQ and LD contributed equally to this work. All authors discussed the results and reviewed the manuscript.

Corresponding authors

Correspondence to Xiao Yang, Yang Si or Shixiong Yi.

Ethics declarations

Conflict of Interest

The authors declare no conflict of interest. The authors declare no competing financial interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (MP4 9909 KB)

Supplementary file2 (DOCX 32267 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qiu, L., Duan, L., Lin, H. et al. Multifunctional and Sprayable 2D MoS2/Silk Sericin Bio-Nanocomposite Dressings with Enhanced Photothermal Effect for Infected Wound Healing. Adv. Fiber Mater. (2024). https://doi.org/10.1007/s42765-024-00407-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s42765-024-00407-7

Keywords

Search

Navigation