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Biomass productivity, synthetic seed preparation, oil extraction and chemical profiling of Scenedesmus obliquus

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Abstract

Microalgae are the quickly growing thalloid organisms and can be used as a renewable source of green energy. The present study focused on the uni-algal culturing, biomass productivity, viability testing of synthetic seed, oil extraction and chemical profiling of the micro-alga, Scenedesmus obliquus. Alga was isolated from leaves and cultured in the Bolds Basal Medium (BBM). The uni-algal culture of the species was developed by serial dilution method. The present study confirmed that BBM was the most successful culture medium for cultivating Scenedesmus obliquus. In the preliminary study, it was found that the biomass productivity of alga was higher in the log stage and later it was decreasing. Synthetic seed test proved that algal beads had more storage viability than pure culture and extend up to years. The percentage of oil in S. obliquus was 54.2 ± 0.0.49%. The GC–MS analysis established that algal oil consists of altogether 37 chemical components with a variety of properties.

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References

  • Bai FW, Anderson WA, Moo-Young M (2008) Ethanol fermentation technologies from sugar and starch feedstocks. Bioethanol Adv 26:89–105

    CAS  Google Scholar 

  • Bligh EG, Dyer WG (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 8:911–917

    Article  Google Scholar 

  • Carla S, Fernandes M (2014) Cultivation scale up and oil extraction from microalgae Monoraphidium obliquus and Secnedesmus obliquus for biodiesel production. Master degree in Biological Engineering Insitute Supperior Techhnico, Lisbon, Portugal

  • Chavan DB, Khobrade CN (2018) Evaluation of fatty acid profile and biodiesel characterization obtained from noval algae Secnedesmus vacuolates X56104. IJAEB 11(1):195–201

    Google Scholar 

  • Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306

    Article  CAS  PubMed  Google Scholar 

  • Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26(3):126–131

    Article  CAS  PubMed  Google Scholar 

  • Demirbas A (2007) Progress and recent trends in biofuels, progress in energy and combustion science. Sci Res 33:1–18

    CAS  Google Scholar 

  • Demirbas A, Demirabus FM (2011) Importance of algae oil as a source of biodiesel. Energy Convers Manag 52:163–170

    Article  Google Scholar 

  • Duffy JE, Canuel EA, Adey W, Swaddle JP (2009) Biofuels: algae. Science 326(5958):1345–1346

    Article  CAS  PubMed  Google Scholar 

  • Hamed I (2016) The evolution and versatility of microalgal biotechnology: a review. Compr Rev Food Sci Food Saf 15(6):1104–1123

    Article  Google Scholar 

  • Harun RMK, Danquah Forde GM (2010) Microalgal biomass as a fermentation feedstock for bioethanol production. J Chem Technol Biotechnol 85:199–201

    CAS  Google Scholar 

  • Hossain ABMS, Salleh A (2008) Biodiesel production from algae as renewable energy. Am J Biochem and Biotechnol 4:250–254

    Article  CAS  Google Scholar 

  • Huber GW, Iborra S, Corma A (2006) Synthesis of tranobliquus ortation fuels from biomass: chemistry, catalysts, and engineering. Chem Rev 106:4044–4098

    Article  CAS  PubMed  Google Scholar 

  • Kulkarni MG, Dalai AK (2006) Waste cooking oil-an economical source for biodiesel: a review. Ind Eng Chem Res 45:2901–2913

    Article  CAS  Google Scholar 

  • Li Y, Horsman M, Wu N, Lan CQ, Dubois CN (2008) Biofuels from microalgae biotechnology progress. Biotechnol Prog 24(4):815–820

    CAS  PubMed  Google Scholar 

  • Liu A, Chen W, Zheng L, Sag L (2011) Identification of high lipid producers for biodiesel production from 43 green algal isolates in China. Prog Nat Sci 21:269–270

    Article  Google Scholar 

  • Liu T, Pang L, Li J, Liu J, Lin W (2014) Isochrysis sp. IOAC724S, a newly isolated lipid enriched marine microalgae for lipid production and optimized cultivation conditions. Biomass Bioenergy 60:32–40

    Article  CAS  Google Scholar 

  • Makareviciene V, Andruleviciute V, Skorupskaite V, Kasperoviciene J (2011) Cultivation of microalgae Chlorella sp. and Scenedesmus sp. as a potentional biofuel feedstock. Environ Res Eng Manag 3(57):21–27

    Google Scholar 

  • Makkar HPS, Tran GV, Heuzé VP, Anker P (2016) Seaweeds for livestock diets: review. Anim Feed Sci Technol 212:1–17

    Article  CAS  Google Scholar 

  • Mansour HAN, Shaaban AS (2010) Algae of soil surface layer of Wadi Al-Hitan protective area (World heritage site), E1-Fayum depression, Egypt. J Am Sci 6:243–255

    Google Scholar 

  • Pereira C, Coutinho I, Soares J, Bessa C, Leao M, Saraiva L (2012) New insights into cancer related protein provided by the yeast model. FEBS J 79(5):697–712

    Article  CAS  Google Scholar 

  • Prescott GW (1962) Algae of the western great lakes area. William C. Brown Company Publishers, Dubuque, p 977

    Google Scholar 

  • Priyadarshani I, Rath B (2012) Commercial and industrial applications of microalgae—a review. JABU 3:89–100

    Google Scholar 

  • Saifullah AZ, Karim A, Ahmad-Yazid A (2014) Microalgae: an alternative source of renewable energy. AJER 3:330–338

    Google Scholar 

  • Santhosh K, Prasanthkumar S, Ray JG (2016) Chlorococcum humicola (Nageli) rabenhorst as a renewable source of bioproducts and biofuel. JPS 5(1):48–57

    Article  CAS  Google Scholar 

  • Scott A, Bryner M (2006) Alternative fuels: rolling out next generation technologies. Chem Week, December 20–27, pp 17–21

  • Shay EG (1993) Diesel fuel from vegetable oils: status and opportunities. Biomass Bioenergy 4:227–242

    Article  CAS  Google Scholar 

  • Shoaw PL, Tang MSY, Nagarajan D, Ling TC, Ooi C, Chang J (2017) A holistic approach to managing microalgae for biofuels applications. IJMS 18:1–34

    Google Scholar 

  • Wang L, Yang B, Du X, Yi C (2008) Optimization of Supercritical extraction of flavionids from Pueraria lobata. Food Chem 108:737–741

    Article  CAS  PubMed  Google Scholar 

  • Yamaguchi K (1997) Recent advances in microalgae bioscience in Japan, with obliquus social reference to utilization of biomass and metaboilites: a review. J Appl Phycol 8:487–502

    Article  Google Scholar 

  • Zancan SR, Trevisan R, Paoletti MG (2006) Soil algae composition under different agro ecosystem in North–Eastern Italy. Agric Ecosyst Environ 112:1–12

    Article  Google Scholar 

Download references

Acknowledgements

Authors would like to express sincere thanks to the Kerala State Council for Science and Technology (KSCSTE), Department of Botany & Phycotechnology Laboratory, Catholicate College, Pathanamthitta for the support to carry out this work.

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Correspondence to Binoy T. T.

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T, B.T., Baby, A., Pillai, A.S. et al. Biomass productivity, synthetic seed preparation, oil extraction and chemical profiling of Scenedesmus obliquus. Vegetos 32, 33–38 (2019). https://doi.org/10.1007/s42535-019-00004-9

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