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Molecular investigations of gene expression analysis in two contrasting genotypes of banana during fusarium wilt (Foc1) infection

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Abstract

Fusarium oxysporum f. sp. cubense is a destructive fungal pathogen causing vascular wilt disease (Panama disease) in banana. The present study examines the pattern of expression of 18 defense-related genes, during Foc1 infection, using two contrasting banana genotypes ‘Calcutta-4’ (Resistant) and ‘Kadali’ (susceptible). The results showed that the expression analysis of 18 selected genes in Resistant genotype ‘Calcutta-4’ showed a peak of upregulation at both 3 and 10 days post inoculation. In all, early induction of the 18 defense genes could be used as indicators of the activation of defense responses in banana genotypes to fusarium wilt infection. The analysis of results indicates early up-regulation of genes involved in signalling and in antioxidants expression, and later, genes involved in cell wall modification and synthesis of secondary metabolites which impart tolerance to Foc1.

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Acknowledgements

Authors thank the Indian Council of Agricultural Research, New Delhi for financial assistance through the ICAR Network Project on Transgenics in Crops: Functional Genomics-Fusarium wilt and drought tolerance in Banana.

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Authors and Affiliations

  1. Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka, 560 089, India

    Pavitra Kotari & Kundapura V. Ravishankar

  2. Department of Biotechnology, Centre for Post Graduate Studies, Jain University, Jayanagar, Bengaluru, Karnataka, 560 011, India

    Pavitra Kotari

  3. Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka, 560 089, India

    Rekha Ajitha

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  1. Pavitra Kotari
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  2. Rekha Ajitha
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  3. Kundapura V. Ravishankar
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Correspondence to Kundapura V. Ravishankar.

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Kotari, P., Ajitha, R. & Ravishankar, K.V. Molecular investigations of gene expression analysis in two contrasting genotypes of banana during fusarium wilt (Foc1) infection. Indian Phytopathology 71, 445–452 (2018). https://doi.org/10.1007/s42360-018-0065-4

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  • DOI: https://doi.org/10.1007/s42360-018-0065-4

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