Skip to main content
SpringerLink
Log in

Identification and confirmation of downy mildew (Pseudoperonospora cubensis Berk. & Curt.) resistance sources in cucumber (Cucumis sativus L.)

  • Research Article
  • Published:
Indian Phytopathology Aims and scope Submit manuscript

Abstract

An experiment was designed to identify the resistance source for downy mildew disease in different genotypes of cucumber. Forty-one cucumber genotypes were screened under natural condition and also under artificial epiphytic condition using detached leaf assay method. Genotype IIHR-438 and Cucumis metuliferus L. showed field resistance with an average PDI of 17.66 and 17.46; AUDPC of 772.24 and 764.48, respectively compared to 73.12 PDI and AUDPC of 3096.64 in highly susceptible genotype of IIHR-389. The disease reaction in selected genotypes of cucumber confirmed by artificial screening was in accordance with disease reaction under natural conditions. Resistant genotype IIHR-438 (14.3 PDI) and C. metuliferus L. (12.8 PDI) had least average PDI as compared to susceptible check Swarna Agethi (58.00 PDI) under artificial condition. Statistical analysis of disease severity data over a period for all the forty-one genotypes using non-linear growth model revealed that 99% variability in disease progression. Screening of genotypes under field conditions, sporulation of pathogen, progress of disease, detached leaf assay and non-linear statistical analysis implied that none of genotypes were found to be immune to downy mildew. Wherein the genotype, IIHR-438 and wild cucumber (C. metuliferus L.) were found resistant to downy mildew disease. Hence, it can be utilized in breeding program to develop resistant cultivar in cucumber against Pseudoperonospora cubensis under tropical conditions of India.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bhutia TL, Munshi AD, Behera TK, Sureja AK, Lal SK, Sinha P (2005) Genetics of yield traits and downy mildew resistance in cucumber (Cucumis sativus L.) Ph.D. thesis, Indian Agricultural Research Institute, New Delhi, p 37

  • Bjoern GK, Kampmann HH (2000) Screening field Riesenschael cucumbers for resistance to downy mildew: management of inter plot interference problems. Acta Hortic 510:77–80. https://doi.org/10.17660/ActaHortic.2000.510.12

    Article  Google Scholar 

  • Call AD (2012) Inheritance of resistance to downy mildew in cucumber (Cucumis sativus L.) PI 197088 and effect of interaction of host plant resistance, fungicides and environment on severity of downy mildew on cucumber. Ph.D. thesis, North Carolina State University, Raleigh, pp 86–88

  • Cohen Y (1977) The combined effects of temperature, leaf wetness, and inoculum concentration on infection of cucumbers with Pseudoperonospora cubensis. Can J Bot 55:1478–1487. https://doi.org/10.1139/b77-174

    Article  Google Scholar 

  • Cohen Y, Eyal H (1977) Growth and differentiation of sporangia and sporangiophores of Pseudoperonospora cubensis on cucumber cotyledons under various combinations of light and temperature. Physiol Mol Plant Pathol 10:93–96. https://doi.org/10.1016/0048-4059(77)90013-3

    Article  Google Scholar 

  • Cohen Y, Petrov L, Baider A (2000) A leaf-disc bioassay for screening cucumbers for resistance to downy mildew. Acta Hortic 510:277–282. https://doi.org/10.17660/ActaHortic.2000.510.46

    Article  Google Scholar 

  • Cohen Y, Meron I, Mor N, Zuriel S (2003) A new pathotype of Pseudoperonospora cubensis causing downy mildew in cucurbits in Israel. Phytoparasitica 31:458–466. https://doi.org/10.1007/BF02979739

    Article  Google Scholar 

  • Criswell AD, Wehner TC, Klosinska U, Kozik E (2008) Use of sporulation and other leaf and vine traits for evaluation of resistance to downy mildew in cucumber. In: Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae. pp 23–27

  • Dhillon N, Pushpinder PS, Ishiki K (1999) Evaluation of landraces of cucumber (Cucumis sativus L.) for resistance to downy mildew (Pseudoperonospora cubensis). Plant Genet Resour Newsl 119:59–61

    Google Scholar 

  • FAO (2016) FAO statistical yearbook. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  • Jeger MJ, Rollinson SLH (2001) The use of the area under disease progress curve (AUDPC) to assess quantitative disease resistance in crop cultivar. Theor Appl Genet 102:32–40. https://doi.org/10.1007/s001220051615

    Article  Google Scholar 

  • Jenkins SF, Wehner TC (1983) A system for the measurement of foliar diseases in cucumbers. Rep Cucurbit Genet Coop 6:10–12

    Google Scholar 

  • Kvalzeth TO (1985) Cautionary note about R 2. Am Stat 39:279–285

    Google Scholar 

  • Lebeda A (1992) Screening of wild Cucumis species against downy mildew (Pseudoperonospora cubensis L.) isolates from cucumbers. Phytoparasitica 20(3):203–210. https://doi.org/10.1007/BF02980842

    Article  Google Scholar 

  • Lebeda A (1999) Pseudoperonospora cubensis L. on Cucumis spp. and Cucurbita spp.—resistance breeding aspects. Acta Hortic 492:363–370. https://doi.org/10.17660/ActaHortic.1999.492.50

    Article  Google Scholar 

  • Lebeda A, Cohen Y (2011) Cucurbit downy mildew (Pseudoperonospora cubensis L.) biology, ecology, epidemiology, host-pathogen interaction and control. Eur J Plant Pathol 129:157–192. https://doi.org/10.1007/s10658-010-9658-1

    Article  Google Scholar 

  • Lebeda A, Prasil J (1994) Susceptibility of Cucumis sativus L. cultivars to Pseudoperonospora cubensis L. Acta Phytopathol 29:89–94

    Google Scholar 

  • Lebeda A, Urban J (2007) Temporal changes in pathogenicity and fungicide resistance in Pseudoperonospora cubensis L. populations. Acta Hortic 731:327–336. https://doi.org/10.17660/ActaHortic.2007.731.44

    Article  CAS  Google Scholar 

  • Mhada M, Ezzahiri B, Benlhabib O (2015) Assessment of downy mildew resistance (Peronospora farinosa) in a Quinoa (Chenopodium quinoa Wild.) Germplasm. Int J Biol Med Res 6(1):4748–4752

    Google Scholar 

  • Nagarajan S, Muralidharan K (1995) Dynamics of plant diseases. Allied publisher, New Delhi

    Google Scholar 

  • Nayak P, Mukherjee AK, Pandit E, Pradhan SA (2018) Application of statistical tools for data analysis and interpretation in rice plant pathology. Rice Sci 25(1):1–18. https://doi.org/10.1016/j.rsci.2017.07.001

    Article  Google Scholar 

  • Neufeld KN, Ojiambo PS (2012) Interactive effects of temperature and leaf wetness duration on sporangia germination and infection of cucurbit hosts by Pseudoperonospora cubensis. Plant Dis 96(3):345–353. https://doi.org/10.1094/PDIS-07-11-0560

    Article  Google Scholar 

  • Neykov S, Dobrev D (1987) Introduced cucumber cultivars relatively resistant to Pseudoperonospora cubensis in Bulgaria. Acta Hortic 220:115–119. https://doi.org/10.17660/ActaHortic.1988.220.16

    Article  Google Scholar 

  • Patil PV (1997) Studies on sunflower rust caused by Puccinia helianthi schw. Ph.D. thesis, University of Agricultural Sciences, Dharwad, p 238

  • Petrov L, Boodert K, Sheck L, Baider A, Rubin E, Cohen Y, Datzir N, Paris HS (2000) Resistance to downy mildew, Pseudoperonospora cubensis in cucumbers. Acta Hortic 510:203–209

    Article  Google Scholar 

  • Ratkowsky DA (1990) Handbook of nonlinear regression models. Marcel Dekker, New York

    Google Scholar 

  • Reddy NS (2002) Biochemical mechanism of downy mildew resistance in musk melon (Cucumis melo L.) caused by Pseudoperonospora cubensis (Berk and Curt) Rostow. M.Sc. thesis, University of Agricultural Sciences, Bangalore, p 21

  • Reshmi (2006) Morphological, molecular diversity and evaluation of developed F1·hybrids for resistance to powdery and downy mildew diseases in Cucumber (Cucumis sativus L.) Ph.D. thesis, University of Agricultural Sciences, Bangalore, p 180

  • Seshadri VS (1986) Cucurbits. In: Bose TK, Som MG (eds) Vegetable crops in India. Studium Press, New Delhi, p 91

    Google Scholar 

  • Siegel S, Castellan ND (1988) Nonparametric statistics for behavioral sciences. McGraw-Hill, New York

    Google Scholar 

  • Sinha P, Prajneshu Varma A (2002) Growth models for powdery mildew development of mango. Ann Plant Prot Sci 10:84–87

    Google Scholar 

  • Thomas CE (1977) Influence of dew on downy mildew of cantaloupe in South Texas. Phytopathology 67:1368–1369

    Article  Google Scholar 

  • Thomas CE (1996) Downy mildew. In: Zitter TA (ed) Compendium of cucurbit diseases. Cornell University Press, New York, pp 25–27

    Google Scholar 

  • Thomas CE, Inaba T, Cohen Y (1987) Physiological specialization in Pseudoperonospora cubensis. Phytopathology 77:1621–1624

    Article  Google Scholar 

  • Urban J, Lebeda A (2006) Fungicide resistance in cucurbit downy mildew—methodological, biological and population aspects. Ann Appl Biol 149:63–75

    Article  CAS  Google Scholar 

  • Van der Plank JE (1968) Plant diseases, epidemics and control. Academic Press, New York

    Google Scholar 

  • Venugopalan R, Vijay N (2015) Nonlinear logistic model for describing downy mildew incidence in grape. J Indian Soc Agric Stat 69(1):19–25

    Google Scholar 

  • Wan H, Zhao Z, Malik AA, Qian C, Chen J (2010) Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in Cucumis. BMC Plant Biol 10:186. https://doi.org/10.1186/1471-2229-10-186

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wehner TC, Shetty NV (1997) Downy mildew resistance of the cucumber germplasm collection in North Carolina field tests. Crop Sci 37:1331–1340

    Article  Google Scholar 

  • Wilcoxson RD, Skovmand B, Atif AH (1975) Evaluation of wheat cultivars for ability to retard development of stem rust. Ann Appl Biol 80:275–281. https://doi.org/10.1111/j.1744-7348.1975.tb01633

    Article  Google Scholar 

Download references

Acknowledgements

The first author is grateful to the Director, Indian Institute of Horticultural Research, Bangalore for their support and facilities provided during this Ph.D. study. The first author thank Dr. Venugopalan R, Indian Institute of Horticultural Research, Bangalore for their helpful suggestions during statistical analysis.

Author information

Authors and Affiliations

  1. Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, 560089, India

    J. C. Bommesh, M. Pitchaimuthu, A. T. Sadashiva & B. Varalakshmi

  2. Division of Plant Pathology, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, 560089, India

    S. Sriram

  3. Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, 560089, India

    K. V. Ravishankar

Authors
  1. J. C. Bommesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Pitchaimuthu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. T. Sadashiva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Sriram
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Varalakshmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. K. V. Ravishankar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Pitchaimuthu.

Ethics declarations

Conflict of interests

The authors declare that they have no conflicts of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bommesh, J.C., Pitchaimuthu, M., Sadashiva, A.T. et al. Identification and confirmation of downy mildew (Pseudoperonospora cubensis Berk. & Curt.) resistance sources in cucumber (Cucumis sativus L.). Indian Phytopathology 71, 337–348 (2018). https://doi.org/10.1007/s42360-018-0061-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42360-018-0061-8

Keywords

Search

Navigation