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Nematicidal Activity of phytocompounds from Piliostigma thonningii Stem Bark Against Meloidogyne javanica

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Abstract

Aim

The study investigated nematicidal activity of phytocompounds isolated from Piliostigma thonningii mature stem bark.

Methods

Compounds were extracted from the stem bark using solid–liquid extraction. Column chromatography was used to separate the phytocompounds and structures were characterized by LC–MS/MS QTRAP. Nematicidal activity of P. thonningii fractions were evaluated against second stage juveniles (J2) of Meloidogyne javanica.

Results

One tetracyclic diterpenoid (1), one glucosinolate (2), and two glycosylated flavonoids (3) and (4) that have strong nematicidal activity against second stage M. javanica juveniles from P. thonningii mature stem bark were identified. Nematicidal activity of the four compounds ranged from 59–99%. Compound 3 exhibited excellent nematicidal activity, 99 ± 1% that was comparable to that of the positive control fenamiphos at 100 mg/ml dose. Its activity increased from 79–99% as concentration was changed from 20 to 100 mg/ml.

Conclusion

The results of the present study shows that P. thonningii mature stem bark consist of phytocompounds that have potent nematicidal activity therefore, they could be associated in an integrated pest management program after further investigations.

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Data Availability

Most of the data obtained have been included in this paper and remaining data can be obtained from the corresponding author upon request.

References

  1. Ighodaro OM, Omole JO (2012) Effects of Nigerian Piliostigma thonningii species leaf extract on lipid profile in Wistar Rats. Int Sch Res Netw ISRN Pharmacol. https://doi.org/10.5402/2012/387942(Article ID 387942)

    Article  Google Scholar 

  2. Dasofunjo K, Asuk AA, Ezugwu HC, Nwodo OFC, Olatunji TL (2013) Aphrodisiac effect of ethanol extract of Piliostigma thonningii leaf on male albino wistar rats. J Appl Pharm Sci 3(10):130–135

    Google Scholar 

  3. Afolayan M, Srivedavyasasri R, Asekun OT, Familoni OB, Orishadipe A, Zulfiqar F, Ibrahim MA, Ross SA (2018) Phytochemical study of Piliostigma thonningii, a medicinal plant grown in Nigeria. Med Chem Res 7:2325–2330. https://doi.org/10.1007/s00044-018-2238-1

    Article  CAS  Google Scholar 

  4. Bello OM, Zack Agbendeh M, Adikwu JG (2013) The comparative studies of phytochemical screening of Piliostigma thonningii root and leaves extract. Asian J of Plant Sci Res 3(6):74–77

    Google Scholar 

  5. Bombardelli E, Morazzoni P, Mustich G (1995) Extracts of Piliostigma thonningii, the use thereof and formulations containing them. Patent number: EP 0685235. European Patent Office, Munich, Germany.

  6. Akinpelu DA, Obuotor EM (2000) Antibacterial activity of Piliostigma thonningii stem bark. Fitoterapia 71:442–443

    Article  CAS  Google Scholar 

  7. Aderogba MA, Okoh EK, Okeke IN, Olajide AO, Ogundaini AO (2006) Antimicrobial and anti-inflammatory effects of Piliostigma reticulatum leaf extract. Int J Pharmacol 2:70–74. https://doi.org/10.3923/ijp.2006.70.74

    Article  Google Scholar 

  8. Dluya T, Dahiru D (2018) Antibacterial activity of Piliostigma Thonningii methanol stem bark extract. Int J Res Pharm Biosci 5:15–20

    Google Scholar 

  9. Asuzu IU, Gray AI, Waterman PG (1999) The anthelmintic activity of d-3-O-methylchiroinositol isolated from Piliostigma thonningii stem bark. Fitoterapia 70:77–79

    Article  CAS  Google Scholar 

  10. Egharevba HO, Folashade KO (2010) Preliminary phytochemical and proximate analysis of the leaves of Piliostigma thonningii (Schumach.) Milne-Redhead. Ethnobot Leafl 14:570–577

    Google Scholar 

  11. Ighodaro OM, Agunbiade SO, Omole JO, Kuti OA (2012) Evaluation of the chemical, nutritional, antimicrobial and antioxidant vitamin profiles of Piliostigma thonningii leaves (Nigerian species). J Med Plants Res 6:537–543

    Article  CAS  Google Scholar 

  12. Eloh K, Kpegba K, Sasanelli N, Koumaglo HK, Caboni P (2020) Nematicidal activity of some essential plant oils from tropical West Africa. Int J Pest Manag 66(2):131–141. https://doi.org/10.1080/09670874.2019.1576950

    Article  CAS  Google Scholar 

  13. Oka K, Shuker S, Kachia N, Trabelcy B, Gerchman Y (2014) Nematicidal activity of Ochradenus baccatus against the root-knot nematode Meloidogyne javanica. Plant Pathol 63:221–231

    Article  CAS  Google Scholar 

  14. Han XZ, Shen T, Lou HX (2007) Dietary polyphenols and their biological significance. Int J Mol Sci 8:950–988

    Article  CAS  Google Scholar 

  15. Aoudia H, Ntalli NG, Aissani N, Yahiaoui-Zaidi R, Caboni P (2012) Nematotoxic phenolic compounds from Melia azedarach against Meloidogyne incognita. J Agric Food Chem 60:11675–11680

    Article  CAS  Google Scholar 

  16. Seanego CT, Ndip RN (2012) Identification and antibacterial evaluation of bioactive compounds from Garcinia kola (Heckel) Seeds. Molecules 17:6569–6584. https://doi.org/10.3390/molecules17066569

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Mureya C (2017) Phytochemical and glucosinolates profiling of Piliostigma thonningii: a plant used to control nematicides. Msc Thesis Bindura University of Science Educaion, Bindura Zimbabwe

  18. Khan R, Naz I, Hussain S, Khan RAA, Ullah S, Rashid MU, Siddique I (2020) Phytochemical management of root knot nematode (Meloidogyne incognita) kofoid and white chitwood by Artemis. Braz J Biol. https://doi.org/10.1590/1519-6984.222040

    Article  PubMed  Google Scholar 

  19. Chin S, Behm CA, Mathesius U (2018) Functions of flavonoids in plant-nematode interactions. Plants 7(4):85. https://doi.org/10.3390/plants7040085(PMid:30326617)

    Article  CAS  PubMed Central  Google Scholar 

  20. Lin SL, Wei T, Lin JF, Guo LQ, Wu GP, Wei JB, Huang JJ, Ouyang PL (2018) Bio-production of baccatin III, an important precursor of paclitaxel by a cost-effective approach. Mol Biotechnol 7:492–505. https://doi.org/10.1007/s12033-018-0090-7

    Article  CAS  Google Scholar 

  21. Wuyts N, Swennen R, Waele DE (2006) Effects of plant phenylpropanoid pathway products and selected terpenoids and alkaloids on the behaviour of the plant-parasitic nematodes Radopholus similis, Pratylenchs penetrans and Meloidogyne incognita. Nematology 8(1):89–101. https://doi.org/10.1163/156854106776179953

    Article  CAS  Google Scholar 

  22. Avato P, D’Addabbo T, Leonetti P, Argentieri MP (2013) Nematicidal potential of Brassicaceae. Phytochem Rev. 12:791–802. https://doi.org/10.1007/s11101-013-9303-7

    Article  CAS  Google Scholar 

  23. Saroj A, Oriyomi OV, Nayak AK, Haider A (2020) Phytochemicals of plant derived essential oils: a novel green approach against pests. Nat Rem Pests Dis Weed Control. https://doi.org/10.1016/B978-0-12-819304-4.00006-3

    Article  Google Scholar 

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Acknowledgements

The authors thank The Tobacco Research Center for LC-MSMS analysis and Bindura University of Science Education for the nematicidal activity assays and laboratory equipment and reagents.

Funding

The work was partially funded by a block grant from the research board of the Bindura University of Science Education.

Author information

Authors and Affiliations

  1. Chemistry Department, Faculty of Science, Bindura University of Science Education, P. Bag 1020, Bindura, Zimbabwe

    Pamhidzai Dzomba & Luke Gwatidzo

  2. Kutsaga Research Station, Airport Ring Road, P. O. Box 1909, Harare, Zimbabwe

    Cleopas Mureya

Authors
  1. Pamhidzai Dzomba
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  2. Cleopas Mureya
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  3. Luke Gwatidzo
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Contributions

PD and CM designed the study and carried out the experimental work. PD and LG wrote the first draft and final draft. LG managed literature and corrected the first draft. All authors read and approved the final draft.

Corresponding author

Correspondence to Pamhidzai Dzomba.

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The authors declared no conflict of interest.

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Dzomba, P., Mureya, C. & Gwatidzo, L. Nematicidal Activity of phytocompounds from Piliostigma thonningii Stem Bark Against Meloidogyne javanica. Chemistry Africa 3, 937–947 (2020). https://doi.org/10.1007/s42250-020-00158-z

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  • DOI: https://doi.org/10.1007/s42250-020-00158-z

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