Prospects for 3D bioprinting of organoids

Bio-Design and Manufacturing (2021)Cite this article

Abstract

Three-dimensional (3D) organoids derived from pluripotent or adult tissue stem cells seem to possess excellent potential for studying development and disease mechanisms alongside having a myriad of applications in regenerative therapies. However, lack of precise architectures and large-scale tissue sizes are some of the key limitations of current organoid technologies. 3D bioprinting of organoids has recently emerged to address some of these impediments. In this review, we discuss 3D bioprinting with respect to the use of bioinks and bioprinting methods and highlight recent studies that have shown success in bioprinting of stem cells and organoids. We also summarize the use of several vascularization strategies for the bioprinted organoids, that are critical for a complex tissue organization. To fully realize the translational applications of organoids in disease modeling and regenerative medicine, these areas in 3D bioprinting need to be appropriately harnessed and channelized.

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(Bioprinted tissue image is reproduced from Lee et al. [62], copyright year 2017, with permission from Dr. Dong-Woo Cho)

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Funding

The manuscript was supported by the Department of Science and Technology (DST), India, DST-ASEAN Grant (CRD/2019/000120). The figures have been created with BioRender.com.

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Affiliations

  1. Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences (ILBS), D1, Vasant Kunj, New Delhi, 110070, India

    Preety Rawal, Dinesh M. Tripathi & Savneet Kaur

  2. School of Biotechnology, Gautam Buddha University, Greater Noida, India

    Preety Rawal

  3. Department of Mechanical Engineering, National University of Singapore, Singapore, Singapore

    Seeram Ramakrishna

Authors
  1. Preety Rawal
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  2. Dinesh M. Tripathi
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  3. Seeram Ramakrishna
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  4. Savneet Kaur
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Contributions

SK and DMT were involved in conceptualization of the study. PR wrote the manuscript and designed the figures. DMT supervised the figure designing. SR and SK reviewed and edited the final draft. All authors approved the manuscript.

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Correspondence to Savneet Kaur.

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This study does not contain any studies with human or animal subjects performed by any of the authors.

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Rawal, P., Tripathi, D.M., Ramakrishna, S. et al. Prospects for 3D bioprinting of organoids. Bio-des. Manuf. (2021). https://doi.org/10.1007/s42242-020-00124-1

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Keywords

  • Three-dimensional bioprinting
  • Bioink
  • Organoid
  • Microfluidics
  • Extracellular matrix (ECM)
  • Biomaterial