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Polycaprolactone–carboxymethyl cellulose composites for manufacturing porous scaffolds by material extrusion

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Abstract

Polycaprolactone–carboxymethyl cellulose composites have been obtained and used to print porous structures by material extrusion. The materials used contained 0, 2 and 5% w/w of the carboxymethyl cellulose additive. These structures have been analyzed in terms of their morphology (including the evaluation of their porosity), mechanical properties under compression load and cell affinity. Cell affinity has been evaluated by culturing sheep mesenchymal stem cells and analyzing their viability by the Alamar Blue® assay at days 1, 3, 6 and 8. The results show that composites samples have similar values of porosity and apparent density than pure polycaprolactone ones. However, samples containing 5% w/w of carboxymethyl cellulose have micropores on the filaments due to a hindered deposition process. This characteristic affects the mechanical properties of the structures, so these ones have a mean compression modulus significantly lower than pure polycaprolactone scaffolds. However, the samples containing 2% w/w of carboxymethyl cellulose show no significant difference with the pure polycaprolactone ones in terms of their mechanical properties. Moreover, the presence of 2% w/w of additive improves cell proliferation on the surface of the porous structures. As complementary information, the flow properties of the composite materials were studied and the power law equations at 210 °C obtained, as this temperature was the 3D printing temperature. These equations can be useful for simulation and designing purposes of other manufacturing processes.

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Acknowledgements

M.E. Alemán-Domínguez would like to express her gratitude for the funding through the PhD Grant Program of Las Palmas de Gran Canaria University (Code of the Grant: PIFULPGC-2014-ING-ARQU-2). The authors would like to thank H2020-MSCA-RISE program, as this work is part of developments carried out in BAMOS project, funded from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No. 734156.

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Authors and Affiliations

  1. Departamento de Ingeniería de Procesos, Universidad de Las Palmas de Gran Canaria, Edificio de Fabricación integrada, Parque Científico-Tecnológico de la ULPGC, 35017, Las Palmas, Spain

    M. E. Alemán-Domínguez, Z. Ortega, A. N. Benítez & L. V. Garzón

  2. Departamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria, Edificio de Fabricación integrada, Parque Científico-Tecnológico de la ULPGC, 35017, Las Palmas, Spain

    Mario Monzón

  3. Institute of Orthopaedic and Musculoskeletal Science, Royal National Orthopaedic Hospital, University College London, Stanmore, London, HA7 4LP, UK

    Sara Ajami & Chaozong Liu

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  1. M. E. Alemán-Domínguez
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  2. Z. Ortega
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Correspondence to M. E. Alemán-Domínguez.

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Alemán-Domínguez, M.E., Ortega, Z., Benítez, A.N. et al. Polycaprolactone–carboxymethyl cellulose composites for manufacturing porous scaffolds by material extrusion. Bio-des. Manuf. 1, 245–253 (2018). https://doi.org/10.1007/s42242-018-0024-z

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