Fabrication of 3D Printed PCL/PEG Polyblend Scaffold Using Rapid Prototyping System for Bone Tissue Engineering Application
- 2 Downloads
Three-dimensional (3D) printing is a novel process used to manufacture bone tissue engineered scaffolds. This process allows for easy control of the architecture at the micro structure. However, the scaffold properties are typically limited in terms of cellular activity at the scaffold surface due to the printed materials properties. In this study, we developed a polycaprolactone (PCL) blended with polyethylene glycol (PEG) 3D printed scaffold using a rapid prototyping system. The manufactured scaffolds were then washed out to form small pores on the surface in order to improve the scaffolds hydrophilicity. We analyzed the resultant material by using Scanning Electron Microscopy (SEM), water absorption, water contact angle, in vitro WST-1, and the Bradford assay. Additionally, cells incubated on the fabricated scaffolds were visualized by Confocal Laser Scanning Microscopy (CLSM). The developed scaffolds exhibited small pores on the strand surface which served to increase hydrophilicity as well as improve cellular proliferation and increase total protein content. Our findings suggest that the presence of small pores on the scaffolds can be used as an effective tool for improving implant cellular interaction. This research indicates that these modified scaffolds can be considered useful for bone tissue engineering applications to improve human health.
Keywordspolycaprolactone polyethylene glycol polyblend 3D printing porosity bone tissue engineering
Unable to display preview. Download preview PDF.
This study was supported by a grant from the Korean Health Technology R & D Project (HI13C1527), Ministry of Health & Welfare and by the Industrial Strategic technology development program (10053020), Ministry of Trade, industry and Energy, Republic of Korea.
- Lee S J, Lee D, Yoon T R, Kim H K, Jo H H, Park J S, Lee J H, Kim W D, Kwon I K, Park S A. Surface modification of 3D-printed porous scaffolds via mussel-inspired polydopamine and effective immobilization of rhBMP-2 to promote osteogenic differentiation for bone tissue engineering. Acta Biomaterialia, 2016, 40, 182–191.CrossRefGoogle Scholar
- Lee S J, Jo H H, Kwon S K, Lee J H, Kim W D, Lee J H, Park S A, Kwon I K. A novel mussel-inspired 3D printedscaffolds immobilized with bone forming peptide-1 for bone tissue engineering applications: Preparation, characterization and evaluation of its properties. Macromolecular Research, 2016, 24, 305–308.CrossRefGoogle Scholar
- Lee S J, Heo D N, Park J S, Kwon S K, Lee J H, Lee J H, Kim W D, Kwon I K, Park S A. Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a 3D printing system. Physical Chemistry Chemical Physics, 2015, 17, 2996–2999.CrossRefGoogle Scholar