Abstract
In this study, two promising novel strains of Trichoderma, T. harzianum KUFA 1051 and T. asperellum KUFA 1067, and Coscinium fenestratum crude extract were tested individually and in combination to control rice dirty panicle under greenhouse conditions. The in planta results showed that the combined use of the antagonists, Trichoderma spp. at 106 spores mL−1 and crude extract of C. fenestratum resulted in better disease reduction and increased rice yield. Treatments with combinations of both antagonists and C. fenestratum extract at 1% showed the greatest disease reduction, a significant reduction by 37.5% compared with the positive controls in rice cultivars, RD 57 and Hom Mali 105 which was higher than that of either of the individual agents alone. Both treatments also showed higher control than that of difenoconazole + propiconazole in rice cv. ‘Hom Mali 105’. The results of this study indicate that the combined use of Trichoderma harzianum KUFA 1051, T. asperellum KUFA 1067 and C. fenestratum crude extract displayed synergistic effects against dirty panicle of rice.
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References
Bae S-J, Mohanta TK, Chung JY, Ryu M, Park G, Shim S, Hong S-B, Seo H, Bae D-W, Bae I, Kim J-J, Bae H (2016) Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biol Control 92:128–138
Chamswarng C, Kumchang T (2012) Efficacy of wettable pellet bioproduct of Trichoderma harzianum strain 01-52 for reducing dirty panicle disease on rice under field conditions. Thai Phytopathology 26:69–80
Charoenrak P, Chamswarng C (2016) Efficacies of wettable pellet and fresh culture of Trichoderma asperellum biocontrol products in growth promoting and reducing dirty panicle of rice. Agric Nat Resour 50:243–249
Cook RJ (2000) Advances in plant health management in 20th century. Annu Rev Phytopathol 38:95–116
Dethoup T, Kumla D, Kijjoa A (2015) Mycocidal activity of crude extracts of marine-derived beneficial fungi against plant pathogenic fungi. J Biopest 8:107–115
Dhamgaye S, Devaux F, Vandeputte P, Khandelwal NK, Sanglard D, Mukhopadhyay G, Prasad R (2014) Molecular mechanisms of action of herbal antifungal alkaloid berberine, in Candida albicans. PLoS One 9:e104554
Fu W, Tian G, Pei Q, Ge X, Tian P (2017) Evaluation of berberine as a natural compound to inhibit peach brown rot pathogen Monolinia fracticola. Crop Prot 91:20–26
Gutiérrez SA, Carmona MA, Reis EM (2010) Methods for detection of Alternaria padwickii in rice seeds. J Phytopathol 158:523–526
Harman GE (2006) Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96:190–194
International Seed Testing Association (1996) International Rules for Seed Testing. The International Seed Testing Association, Zurich
Ismael JHS, Mahmood SH (2016) Management of tomato damping-off using natural plant extracts, Trichoderma harzinum and selected fungicides in Penjween, Sulaimani governorate, Kurdistan, Iraq. Malays Appl Biol 45:35–48
Melo PAFA, Alves EU, Martins CC, Anjos Neto AP, Pinto KMS, Araújo LR, Vieira CP, Nascimento LC (2016) Extracts of Caesalpinia ferrea and Trichoderma sp. on the control of Colletotrichum sp. transmission in Sideroxylon obtusifolium seeds. Rev Bras Plantas Med 18. https://doi.org/10.1590/1983-084x/15_191
Mutawila C, Halleen F, Mostert L (2015) Development of benzimidazole resistance Trichoderma strains for the integration of chemical and biocontrol methods of grapevine pruning wound production. BioControl 60:387–399
Muthayya A, Sugimoto JD, Montgomery S, Maberly GF (2014) An overview of global rice production, supply, trade, and consumption. Ann N Y Acad Sci 1324:7–14
Muthukumar A, Eswaran A, Nakkeeran S, Sangeetha G (2010) Efficacy of plant extracts and biocontrol agents against Pythium aphanidermatum inciting chili damping-off. Crop Prot 29:1483–1488
Naveenkumar R, Muthukumar A, Sangeetha G, Mohanapriya R (2017) Developing eco-friendly biofungicide for the management of major seed borne diseases of rice and assessing their physical stability and storage life. C R Biol 340:214–225
Pascale A, Vinale F, Manganiello G, Nigro M, Lanzuise S, Ruocco M, Marra R, Lombardi N, Woo SL, Lorito M (2017) Trichoderma and its secondary metabolites improve yield and quality of grapes. Crop Prot 92:176–181
Pertot I, Giovannini O, Benanchi M, Caffi T, Rossi V, Mugnai L (2017) Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine. Crop Prot 97:85–93
Ruano-Rosa D, Prieto P, Rincon AM, Gomez-Rodríguez MV, Valderrama R, Barroso JB, Mercado-Blanco J (2016) Fate of Trichoderma harzianum in the olive rhizosphere: time course of the root colonization process and interaction with the fungal pathogen Verticillium dahliae. BioControl 61:269–282
Singburaudom N (2015) The alkaloid berberine isolated from Coscinium fenestratum is an inhibitor of phytopathogenic fungi. J Biopest 8:28–36
Tamilselvi S, Venkatasubramanian P, Kannan KP, Vasanthi NS (2014) Studies on estimation of berberine and antimicrobial activity of different extracts of Berberis aristata DC. Asian J Microbiol Biotechnol Environ Sci 16:669–672
Wu Q, Zhang LD, Xia H, Yu CJ, Dou K, Li YQ, Chen J (2017) Omics for understanding synergistic action of validamycin a and Trichoderma asperllum GDFS1009 against maize sheath blight pathogen. Sci Rep 7. https://doi.org/10.1038/srep40140
Zehra A, Meena M, Dubey MK, Aamir M, Upadhyay RS (2017) Synergistic effects of plant defense elicitors and Trichoderma harzianum on enhanced induction of antioxidant defense system in tomato against fusarium wilt disease. Bot Stud 58:44. https://doi.org/10.1186/s40529-017-0198-2
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This work was financially supported by the Kasetsart University Research and Development Institute under the project “Searching for antifungal compounds from medicinal plants against plant pathogenic fungi for development natural fungicides”.
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Kaewsalong, N., Songkumarn, P., Duangmal, K. et al. Synergistic effects of combinations of novel strains of Trichoderma species and Coscinium fenestratum extract in controlling rice dirty panicle. J Plant Pathol 101, 367–372 (2019). https://doi.org/10.1007/s42161-018-0191-y
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DOI: https://doi.org/10.1007/s42161-018-0191-y