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Immune responses in Brazilian banana determining the pathogenic differences between the physiological races 1 and 4 of Fusarium oxysporum f. sp. cubense

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Abstract

To study whether the immunoreactions of banana contribute the pathogenic differences between physiological races 1 (Foc1) and 4 (Foc4) of Fusarium oxysporum f. sp. cubense (Foc), we monitored the resistance reaction of Brazilian banana plants treated respectively by inoculation with Foc1 and Foc4, coculture in the medium with pure oligogalacturonides (OGs), and pretreatment with OGs before the inoculation with Foc4 (OGs-Foc4). Our results showed that all four treatments could induce the up-regulated expression of the defense-related genes and increase the callose and flavonoid contents. Moreover, the range of the increase induced by Foc1 was higher than that induced by Foc4, and the resistance against Foc4 was stronger in plants pretreated with OGs than in plants that were not pretreated with OGs. We also found that both Foc1 and Foc4 could induce the H2O2 outburst, including the two outbursts of Foc4 and one outburst of Foc1. Although no significant change of H2O2 was induced by OGs alone, the contents of H2O2 production in plants treated with OGs-Foc4 were lower than in plants treated with Foc4 alone. This result indicates that the pretreatment with OGs can inhibit the H2O2 production during the Foc4 infection, resulting in a reduction in the occurrence of necrotic hypersensitive reaction. These results suggest that the immune reactions of banana are involved in the regulation of different pathogenicities of Foc1 and Foc4. Moreover, OGs may play an important intermediate role in inducing the immune responses.

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Acknowledgements

The study was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-31-09) in China.

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Authors and Affiliations

  1. State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Huiyun Fan, Zhaoxi Lei, Honghong Dong & Huaping Li

  2. Institute of Tropical Bioscience&Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China

    Dengbo Zhou

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  1. Huiyun Fan
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  2. Zhaoxi Lei
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Correspondence to Huaping Li.

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Fan, H., Lei, Z., Dong, H. et al. Immune responses in Brazilian banana determining the pathogenic differences between the physiological races 1 and 4 of Fusarium oxysporum f. sp. cubense. J Plant Pathol 101, 225–234 (2019). https://doi.org/10.1007/s42161-018-0165-0

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