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Journal of Plant Pathology

, Volume 100, Issue 2, pp 325–325 | Cite as

First report of Diplodia seriata causing fruit rot of loquat in Pakistan

  • Muhammad Fahim Abbas
  • Farah Naz
Disease Note

Loquat (Eriobotrya japonica Lindl.) is achieving significant importance in Pakistan and 98% Pakistani commercial loquat plantations are located in Punjab (31.0° N, 72.0° E) and Khyber Paktoon Khawa (34.0° N, 71.32° E). During the survey of loquat orchards, 6 to 13 mm irregular brownish dry spots were observed on loquat fruits. The fruits were incubated at 22 °C for two weeks and spots turned to black or dark brown. Surface of symptomatic fruits was disinfected with cholrox and planted them in Czapek Dox Agar. Dark gray mycelium was observed on both side of plate surface at 26 °C. Aseptate brown conidia with the apex broadly rounded and the base rounded or truncate, and 22 × 10 μm (n = 49) in size were observed. Molecular identification of Diplodia seriata was performed with the primers ITS1 and ITS4 and nucleotide BLAST analysis of PAK20, PAK21 and PAK33 (GenBank Accession No. KR822800, KR822801 and KU052580) showed 99% to 100% identity with D. seriata (KJ463386 and KT447249). Four mm fungal mycelial plug (one plug per fruit; n = 9) of PAK20, PAK21 and PAK33 was transferred to skin wounds on superficially disinfected loquats whereas wounded but not inoculated fruit were used as controls. The experiment was repeated thrice and fruits were incubated 7 to 21 days at 20 °C. Nineteen to 98 mm lesions were observed on inoculated fruits and no lesion was recorded on controls. The fungus was re-isolated from inoculated fruits and compared with mother cultures. D. seriata is a broadly spread pathogen causing blight, cankers, dieback and fruit rots in vines and many fruit trees (Bobev et al. 2008; Choudhury et al. 2014; González-Domínguez et al. 2014; Kim et al. 2016). To our knowledge, this is the first report of D. seriata causing loquat fruit rot in Pakistan.

References

  1. Bobev S, Lopes J, Phillips A (2008) First report of Diplodia seriata causing shoot blight and cankers of Cotoneaster salicifolius in Bulgaria. Plant Dis 92:976–976CrossRefGoogle Scholar
  2. Choudhury R, Modi P, Hanstad J, Elkins R, Gubler W (2014) First report of Diplodia seriata causing pear branch canker dieback in California. Plant Dis 98:688–688CrossRefGoogle Scholar
  3. González-Domínguez E, León M, Armengol J (2014) Branch cankers of loquat caused by botryosphaeriaceae spp. in Spain. In: IV Int Symp Loquat 1092: 297–300Google Scholar
  4. Kim YK, Kwak JH, Aguilar CG, Xio CL (2016) First report of black rot on apple fruit caused by Diplodia seriata in Washington state. Plant Dis 100:1499CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  1. 1.Department of Plant PathologyPir Mehr Ali Shah Arid Agriculture UniversityRawalpindiPakistan

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