Molecular and biological characterization of an isolate of capsicum chlorosis virus from IRAN
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A virus-like disease characterized by chlorotic ring spot symptoms on the leaves was observed in plants of Rudbeckia sp. in Mahallat (Markazi province, Iran). The viral agent was mechanically transmitted to Nicotiana benthamiana and designated RM. A PCR fragment was amplified from inoculated N. benthamiana plants, using generic tospovirus primers derived from the nucleocapsid gene of the Asian 1 clade of tospoviruses. Sequence analysis of this fragment revealed 88–97% nucleotide identity and 91–99% amino acid identity with those of capsicum chlorosis virus (CaCV) isolates. The entire S RNA, NSM gene and partial L gene were sequenced revealing typical tospoviral genomic features. The sequence of the nucleocapsid (N) protein showed the closest relationship (97.8% identity) with that of the gloxinia isolate (HT-1) of CaCV from the USA. The rudbeckia virus was therefore identified as an isolate of CaCv. The virus was able to infect a limited number of plant species tested, showing symptoms distinct from those elicited by other CaCV isolates. To our knowledge, this is the first report of an isolate of CaCV in Iran and the Middle East.
KeywordsTospoviridae Orthotospovirus Rudbeckia Iran
This study was partially supported by Ornamental Plant Research Center of Iran.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest.
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