Effect of b values and size of region of interest on apparent diffusion coefficient measurement and its reproducibility in liver diffusion-weighted MRI



To investigate the effect of b value and size of region of interest (ROI) on apparent diffusion coefficient (ADC) measurement and its reproducibility in liver diffusion-weighted imaging (DWI).


Thirty-six volunteers underwent liver DWI twice with b values of 0, 100, 500, and 800 s/mm2. ADCs were measured with ROI of 50 mm2 on ADC maps generated with different b values of (0, 100), (0, 500), (0, 800), (0, 100, 500), (0, 100, 800), (0, 500, 800), and (0, 100, 500, 800) s/mm2. ADCs from b values of (0, 800) s/mm2 were measured with 4 ROI sizes (50, 100, 200 and 300 mm2). ANOVA analysis was used to compare differences of ADCs among different ROI sizes and different combined b values. Bland–Altman method was used to assess reproducibility of ADC measurement.


ADCs with larger ROI size were slightly higher than those with smaller one, while no statistical difference was found (P > 0.05). And reproducibility of ADC measurement with different ROI sizes was comparable (LOA 7.0–8.2% for right lobe, 14.15–17.4% for left lobe). ADCs statistically decreased with increased maximum b values (P < 0.05). ADC measurement achieved the best reproducible with maximum b value of 800 s/mm2 regardless of the number of b values.


The b values influence ADC measurement and its reproducibility, while the ROI sizes do not affect them. Two b values of (0, 800) s/mm2 and ROI of 50 mm2 are recommended for liver ADC measurement.

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Correspondence to Huang Yucun.

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Yucun, H., Liling, X., Zhi, C. et al. Effect of b values and size of region of interest on apparent diffusion coefficient measurement and its reproducibility in liver diffusion-weighted MRI. Chin J Acad Radiol (2021). https://doi.org/10.1007/s42058-021-00053-7

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  • Liver diffusion-weighted imaging
  • Apparent diffusion coefficient
  • b values
  • Region of interest
  • Reproducibility