Abstract
Candida antarctica lipase B (CALB) was cross-linked efficiently, using a reagent containing glutaraldehyde (GA), paraformaldehyde (PA), and a polylysine reagent. For the reaction under micro-flow conditions, CALB was immobilized on the inner surface of a polytetrafluoroethylene (PTFE) tube, by flowing the cross-linking reagents in an appropriate flow rate. The immobilized CALB formed a membrane-like structure on the inner surface of the tube and showed catalytic activity for transesterification. Although the enzyme activity was not high at the immobilized CALB, the productivity of micro-flow reaction using immobilized CALB was higher than that of batch-wise reaction using free CALB.
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References
Polaina J, MacCabe AP (2010) Industrial enzymes. Springer, Dordrecht
Casas-Godoy L, Meunchan M, Cot M, Duquesne S, Bordes F, Marty A (2014) J Biotechnol 180:30–36
Chang SW, Shaw JF, Yang KH, Shih IL, Hsieh CH, Shieh CJ (2005) Green Chem 7:547–551
Gutmann B, Cantillo D, Kappe O (2015) Angew Chem Int Ed 54:6688–6728
Heintz S, Mitic A, Ringborg RH, Krühne U, Woodley JM, Gernaey KV (2016) J Flow Chem 6:18–26
Jime’nez-Gonza’lez C, Poechlauer P, Broxterman QB, Yang BS, Ende D, Baird J, Bertsch C, Hannah RE, Dell’Orco P, Manley J (2011) Org Process Res Dev 15:900–911
Novak U, Lavric D, Žnidaršič-Plazl P (2016) J Flow Chem 6:33–38
Wohlgemuth R, Plazl I, Žnidaršič-Plazl P, Gernaey KV, Woodley JM (2015) Trends Biotechnol 33:302–314
Žnidaršič-Plazl P (2017) J Flow Chem 7:111–117
Fu H, Dencic I, Tibhe J, Sanchez Pedraza CA, Wang Q, Noël T, Meuldijk J, Croon M, Hessel V, Diels L (2012) Chem Eng J 207:564–576
Denčić I, Noël T, Meuldijk J, Croon M, Hessel V (2013) Eng Life Sci 13:326–343
Honda T, Miyazaki M, Nakamura H, Maeda H (2005) Chem Commun:5062–5064
Honda T, Miyazaki M, Nakamura H, Maeda H (2006) Adv Synth Catal 348:2163–2171
Doukyu N, Ogino H (2010) Biochem Eng J 48:270–282
Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2007) Enzym Microb Technol 40:1451–1463
Sheldon RA (2007) Adv Synth Catal 349:1289–1307
Yoshimura Y, Osaki S, Miyake Y, Mori H (2013) Reports of Industrial Technology Center of Wakayama Prefecture, pp 11–12
Yoshimura Y, Tsuchitani A, Mori M, Osaki S, Miyazaki T, Mori H (2016) The 96th CSJ Annual Meeting, 3PA-231
Bolivar JM, Eisl I, Nidetzky B (2015) Catal Today 259:66–80
Wold F (1972) Methods Enzymol 25:623–651
Karnovsky MJ (1965) J Cell Biol 27:137A–138A
Barbosa O, Torres R, Ortiz C, Fernandez-Lafuentec R (2012) Process Biochem 47:766–774
Uppenberg J, Hansen MT, Patkar S, Jones TA (1994) Structure 2:293–308
Ishii M (1989) WO Patent 1988002775 A1
Alotaibi M, Manayil JC, Greenway GM, Haswell SJ, Kelly SM, Lee AF, Wilson K, Kyriakou G (2018) React Chem Eng. Advance Article
Nie K, Xie F, Wang F, Tan T (2006) J Mol Catal B Enzym 43:142–147
Bai YX, Li Y, Feng YY, Yi LX (2006) Process Biochem 41:770–777
Chen B, Hu J, Miller EM, Xie W, Cai M, Gross RA (2008) Biomacromolecules 9:463–471
Chulalaksananukul W, Condoret JS, Combes D (1992) Enzym Microb Technol 14:293–298
Yadav GD, Trivedi AH (2003) Enzym Microb Technol 32:783–789
Henderson JW, Ricker RD, Bidlingmeyer BA, Woodward C (2000) Agilent Technologies, Inc., 5980-1193E
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Yoshimura, Y., Saito, A., Mori, M. et al. Immobilization of lipase on the surface of a micro tube and continuous transesterification. J Flow Chem 8, 45–50 (2018). https://doi.org/10.1007/s41981-018-0006-5
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DOI: https://doi.org/10.1007/s41981-018-0006-5