In vitro studies of α-TCP and β-TCP produced from Clinocardium ciliatum seashells

Abstract

In this study, α-TCP and β-TCP were successfully produced via a mechanochemical method from aragonite structures, Clinocardium ciliatum seashells, at 850 °C, 1000 °C, and 1200 °C sintering temperatures. The characterization of the obtained materials was carried out via FT-IR, SEM/EDX, BET, XRD, ICP-OES analysis. Samples were soaked for 21 days in simulated body fluid (SBF) for a bioactivity test. Additionally, MTT assay was applied to determine the cell viability of samples. Bioactivity in vitro tests showed that bone-like hydroxyapatite formed when the α-TCP and β-TCP were soaked in SBF. TCP bioceramics had a noncytotoxicity effect on SAOS-2 osteoblast-like cells and cell viability increased in 1 to 7 days. The produced bioceramics have ideal pore sizes and properties that are suitable for supporting biological activities. Tris-HCl buffer solution was used to obtain the level of biodegradation. It was seen that α-TCP exhibited better dissolution features than β-TCP.

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Acknowledgments

Special thanks to ArelPOTKAM (Polymer Technologies and Composite Application and Research Center) where the synthesis of bioceramics took place.

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Correspondence to Yesim Muge Sahin.

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Sahin, Y.M., Orman, Z. & Yucel, S. In vitro studies of α-TCP and β-TCP produced from Clinocardium ciliatum seashells. J Aust Ceram Soc 56, 477–488 (2020). https://doi.org/10.1007/s41779-019-00355-1

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Keywords

  • Bioactivity
  • Bone regeneration
  • SBF
  • Seashell
  • TCP
  • Tris