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Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae)

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Abstract

This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14 days, respectively. After confirming that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 µg g−1 were prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were > 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial foods (0.5 and 2.0 µg g−1 of Pb) during 7 and 14 days, respectively. The observed acceptance of these foods by crabs at both Pb concentrations was of 100%.

Article Highlights

  • An artificial food was prepared to assess bioaccumulation in the blue crab

  • Lead (Pb) was chosen as the metal of interest

  • The acceptance of artificial food was evaluated

  • In the contaminated artificial foods, Pb was determined

  • It was concluded that this food can be applied in metal exposure assays

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Acknowledgements

The authors would like to thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for the financial support (Processo FAPESP n. 2014/01576–6).

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Authors and Affiliations

  1. Núcleo de Estudos em Poluição e Ecotoxicologia Aquática (NEPEA), UNESP-Univ Estadual Paulista, Campus do Litoral Paulista, Praça Infante Dom Henrique snº, Parque Bitaru, São Vicente, SP, CEP 11330-900, Brazil

    Isabella C. Bordon, Bruno Galvão de Campos, Paloma Kachel Gusso-Choueri, Giuliana Seraphim de Araujo & Denis Moledo de Souza Abessa

  2. Laboratório de Ecologia e Comportamento Animal (Labecom), UNESP-Univ Estadual Paulista, Campus do Litoral Paulista, Praça Infante Dom Henrique snº, Parque Bitaru, São Vicente, SP, CEP 11330-900, Brazil

    Caio Akira Miyai

  3. Biomedical and Biological Sciences Institute (ICB), USP- University of São Paulo, Av. Prof. Lineu Prestes, 1524-Cidade Universitária, São Paulo, SP, CEP 05508-900, Brazil

    Andrews Krupinski Emerenciano & José Roberto Machado Cunha da Silva

  4. Chemical and Environmental Technology Centre, IPEN- Nuclear and Energy Research Institute, Avenida. Professor Lineu Prestes 2242, Cidade Universitária, Sao Paulo, SP, CEP 05508-000, Brazil

    Marycel Elena Barboza Cotrim

  5. Research Reactor Centre, IPEN- Nuclear and Energy Research Institute, Avenida. Professor Lineu Prestes 2242, Cidade Universitária, Sao Paulo, SP , CEP 05508-000, Brazil

    Déborah Inês Teixeira Favaro

Authors
  1. Isabella C. Bordon
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  2. Bruno Galvão de Campos
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  3. Paloma Kachel Gusso-Choueri
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  4. Caio Akira Miyai
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  5. Giuliana Seraphim de Araujo
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  6. Andrews Krupinski Emerenciano
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  7. José Roberto Machado Cunha da Silva
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  8. Marycel Elena Barboza Cotrim
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  9. Déborah Inês Teixeira Favaro
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  10. Denis Moledo de Souza Abessa
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Corresponding author

Correspondence to Isabella C. Bordon.

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The authors declare no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Bordon, I.C., de Campos, B.G., Gusso-Choueri, P.K. et al. Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae). Int J Environ Res 13, 431–434 (2019). https://doi.org/10.1007/s41742-019-00170-3

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  • DOI: https://doi.org/10.1007/s41742-019-00170-3

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